LIPIDS

John Joseph Balladolid Performer

Kenny Jay-R Basabe Observer
Faye Christine Gapayao Recorder
Jake LobasObserver

Date Performed: November 20,2019

INTRODUCTION
* Which was more healthy between Plant and
Animal oils?
* What are lipids?
* Where are the good fats comes from?
* What is the difference of saturated oils and
unsaturated oils?
OBJECTIVES
1. Learn how to test for the unsaturated
character of plant oil,
2. Learn how to test for saturated character of
plant oil,
3. Understand the rate of unsaturated fatty
acids in our day to day living.
HYPOTHESIS

Saturated oils are solid in room temperature and

unsaturated oils are liquid in room temperature.
PROCEDURE
A. Test for unsaturation of fatty acids.

Shake
Shake the
the stoppered
stoppered test
test
Add
Add 55 mL
mL of
of acetic
acetic acid
acid tubes
tubes then stand for 1
then stand for 1
Fill
Fill test
test tubes
tubes 1-5
1-5 with
with 1-
1- solution
solution to each of
to each of the
the min
min and
and add
add 1010 drops
drops of
of
2 mL of the samples.
2 mL of the samples. five
five test tubes then add
test tubes then add starch solution to each
starch solution to each
2-4
2-4 drops
drops iodine
iodine solution.
solution. test
test tubes
tubes then
then shake
shake
again and then observe.
again and then observe.
B. Degree of unsaturation

Add 1 mL of
Add 2-3 drops
Fill test tubes ethanol then Shake then
bromine
1-5 with 1 mL stopper each note any
water to each
of the oil test tubes change in
of the test
samples. and then color.
tubes.
shake.
DATA AND RESULTS
A. TEST FOR UNSATURATION OF FATTY ACIDS
Table 1. Test for unsaturation of five different oil samples using iodine and
starch solution.

SAMPLES HYPOTHESIS OBSERVATION OBSERVATION FIRST TEST

(+) positive Iodine an starch Oil sample with RESULT
(-) negative solution after iodine and (+), (-), (D)
(D) doubtful test starch after test

Argan oil D Pink color Has pink -

above, color above
cloudish with a
white at the measure of
bottom, 2.2 cm in
bluish color height, dark
at the center violet below
and 3-5 cm in
height.
 Corn oil + Cloudish white Has orange +
at the bottom, color above
has dark orange with 19 cm in
color above height
Coconut oil + Color yellow Dark-red- +
below, has brownish color
bubbles. Bloody above, 1cm in
red color at the height, black
top, pink color color below
at the center with 4.8 cm in
height
Virgin + Yellow color in 1.5 cm scarlet +
coconut oil the bottom color above and
with red 4 cm bnlack
bubbles. Has color above.
color red at the
top
Canola oil - White at the 1.3 cm light +
bottom, red- orange above,
orange at the 4.2 cm dark
top, has few violet color at
bubbles at the the bottom
center.
B. DEGREE OF UNSATURATION OF OILS
Table 1: Test for unsaturation of five different oil samples using ethanol and bromine
water

SAMPLES INITIAL EFFECT OF EFFECTS OF FIRST TEST

APPEARANCE ADDING ADDING RESULT
ETHANOL BROMINE (+), (-), (D)

Argan Oil transparent Whitish in color Cloudish in +

color with
coagulate
below
Corn oil Yellow in Dirty White Cloudish yellow +
color at the bottom
and white at
the top and
center
Coconut oil Whitish in White Becomes yellow +
color then turns
cloudish white
with coagulates
after shaking
 Virgin transparent Bluish in color Yellow at first +
coconut oil and has then turns
bubbles and white after it
spots has been
shake.
Canola oil White with Yellowish and Dark yellow +
slight yellow when shake, at first then
in color it turns with turned white
many and has
bubbles/spots bubbles after
shaking.
POST LABORATORY QUESTIONS
1. What are the most common fatty acids found in sunflower, corn,
coconut and canola oils?
2. What are the most common fatty acids founds in animals?
3. What are essentially fatty acids?
4. Using your results, which plant oils contain polyunsaturated fatty
acids?
5. What is meant by, a saturated oil? Unsaturated oil?
Polyunsaturated oil?
6. Why is it important to dissolve the oil in ethanol before adding
the bromine water?
7. Describe how the test you have carried out shows whether an oil
is saturated or unsaturated?
8. Why do you suppose the body is better at metabolizing
unsaturated oils and fats?