DENTINOGENESIS

Dr Saqlain Bin Syed Gilani

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DENTINOGENESIS

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 Dentin formation begins when the tooth germ reaches
the bell stage
 Enamel organ is formed
 Internal enamel epithelium ready to secrete the matrix
 Dental papilla becomes the pulp
DIFFERENTIATION OF ODONTOBLASTS
 Dentin forming cells arise from ectomesenchyme of
dental papilla
 Neural crest in origin
 Internal Enamel Epithelium expresses signaling
molecules and growth factors
 Influences the dental papilla
 • Small & undifferentiated
• Nucleus lying in the center
Dental Papilla

• Separated from Dental Papilla by a layer an acellular layer

• After the reversal of polarity changes occur in D.P
IEE

• Elongates, forms preodontoblasts  odontoblasts

• Acellular zone gradually disappears with the increase in size of
odontobalsts
Ectomesen- • Newly formed cells are polarized with nucleus placed away from IEE
-chymal cells
A B C D
 MANTLE DENTIN
Post cell differentiation, organic matrix has be to be formed

First sign of dentin formation is the appearance of large collagen fibrils

(0.1 – 0.2mm) (Von Korff’s fibers). Type III with fibronectin

These fibers originate deep among odontoblasts, extend towards IEE and
fan out in the structurless ground substance

As Odontoblasts continue to increase in size, they produce Type I

collagen fibers
• Arranged parallel to the future EDJ

Mantle Predentin is formed

 Coincident with collagen deposition, plasma membrane
of odontoblasts adjacent to ameloblasts extend their
processes into the extra cellular matrix
 Enamel Spindles
 • As odontoblasts form these processes
• They bud off small vesicles near basal lamina
Matrix
Vesicles

• Cell process (odontoblast process) is formed

• O.P left behind in the matrix as odontoblast moves pulpally
Odontoblast
cell Process

• Mineral phase first appears in the matrix vesicles as single crystals

(seeded by phosphplipids)
• These crystals grow in size and rupture out as clusters, fusing with the
Mineral adjacent clusters and form a continuous layer of mineralized matrix
Phase
 Deposition of mineral lags behind the formation of
organic matrix
 Non collagenous proteins regulate mineralization

(A)Predentin & A
(B) Odontoblast
process
B
VASCULAR SUPPLY DURING
MINERALIZATION

Subodontoblastic Layer
Mantle dentin

Migrate B/W
Odontoblasts Circumpulpal
& dentin
Endothelial lining fenestrate

Retreat & lining becomes Completion of

Dentinogenesis
Continuous
CONTROL OF MINERALIZATION
 Odontoblasts control initiating mineralization by matrix
vesicles & regulating proteins
 Calcium L type have been demonstrated in the basal
lamina
 Blocking these Ca channels can affect mineralization
 Presence of alkaline phosphatase and calcium
adenosinetriphosphatase activity in the distal end aids in
transport of mineral ions
PATTERN OF MINERALIZATION

Pattern
Globular
Linear
ROOT DENTINOGENESIS
 Hertwig root sheath epithelial cells initiate odontoblast
differentiation
 Some differences from coronal dentinogenesis

 Outermost layer (similar to mantle dentin in crown) has

different collagen fiber orientation
 Phosphoprotein content may also differ
SECONDARY & TERTIARY
DENTINOGENESIS
 Secondary;
 Post root formation, by the same odontoblasts
 Continuation of primary dentin but much slower in pace
 May have crowding of odontoblast cells or absence (in case
of death)
 Tertiary;
 Remaining odontoblasts or odontoblast like cells
 Rate of deposition depends on the rate of injury
 In case of rapid deposition
 Irregular tubular structure maybe formed
REFERENCES
 Berkovitz, pages: 329 – 338
 Ten Cate’s 8th Edition, pages: 170 - 204