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Immunoglobulins, Immune Response: Martin Liška
Immunoglobulins, Immune Response: Martin Liška
Immunoglobulins, Immune Response: Martin Liška
immune response
Martin Liška
1. The structure of
immunoglobulins
2. Isotypes
• (in principle) classes of antibodies distinguished on
the basis of H chain structure differences
genes on chromosome 22
b/ genes encoding H chain
• more complicated
• localized on chromosome 14
• V, D, J, C segments (genes encoding
individual segments contain more regions
compared with L chains)
• during completion of V/D/J exon, gene
rearrangement occurs
Development of B lymphocytes
• Lymphoid progenitor → pro-B cells
• During maturation from pro-B cells into pre-B cells:
Ig genes of the heavy chain recombine; pre-B cells
express pre-BCR
• During maturation from pre-B cells into B cells: Ig
genes of the light chain recombine
• Immature B cells express membrane IgM
• Mature B cells express membrane IgM and IgD =
BCR and are able to respond to antigen in peripheral
lymphoid tissues
Mechanisms contributing to antibody
diversity:
• chance recombinations
• imprecise joining of V, D, J genes
• N-region additions
• extensive mutations involving variable-
region genes after antigen exposure
Isotype switching
• during the immune response, plasma cells
switch from producing IgM to IgG or to
another Ig class (IgA, IgE)
• the switch involves a change in the H-chain
constant domains (CH)
• no change in antigen-binding specificity !
(no alteration in the L chain or in the variable
portion of H chain)
Allelic exclusion