PARAFFIN AND PLASTIC

EMBEDDING METHOD
Hafsa bano
serial sectioning is essential to a quality histological study
this method allows to trace changes in the explant and
make a three dimensional
many protocols regarding embedding are available
The process to embed a tissue perform on a hot plate
EMBEDDING:

it is a process in which tissue is surrounded

by a medium such as agar, paraffin wax, plastic which
when solidify will provide sufficient external support
during sectioning

In general there are two common embedding method

that will allow for serial sectioning

Paraffin wax
Glycol methacrylate(GMA)
this method is specially useful for large explants
the cost associated with paraffin embedding method is
relatively low in comparison to the plastic embedding
method
CUTTING:
paraffin block are cut by microtome

MICROTOME:
microtome is a mechanical instrument used to cut biological specimens into very thin segments about 6
micrometer for microscopic examination
they may be
>automated
>semi automated
>manual
 Tissue section placed in a
waterbath Take up on a slide

SLIDE IS READY FOR STAINING