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Bacterial Crowth Curve
Bacterial Crowth Curve
Kalavati Prajapati
Assistant Professor
Department of Microbiology,
HVHP Institute of Post Graduate
Studies & research, Kadi
Increase in cellular constituents that may
result in:
– increase in cell number
• e.g., when microorganisms reproduce by budding
or binary fission
– increase in cell size
• e.g., coenocytic microorganisms have nuclear divisions that are
not accompanied by cell n divisions
• microbiologists usually study population growth rather than
growth of individual cells
Definition: Growth may be defined as an increase in
cellular constituents. It leads to a rise in cell number
when microorganisms reproduce by processes like
budding or binary fission.
The growth of microorganisms reproducing by
binary fission can be plotted as the logarithm
of the number of viable cells versus the
incubation time
The resulting curve has four distinct phases
1. Lag Phase
2. Log Phase (Exponential Phase)
3. Stationary Phase (idio phase)
4. Death Phase (Decline phase)
Cell division does not take place
Cell synthesizing new components
– e.g., to replenish spent materials
conditions
Varies in length
Death
– irreversible loss of ability to reproduce
2--->2¹--->2²--->2³-------------
Assume that a culture tube is inoculated with one cell that divides every 20 minutes
The population will be 2 cells after 20 minutes, 4 cells after 40 minutes, and so
forth. Because the population is doubling every generation, the increase in
population is always 2n where n is the no of generation.
2. Physiologically the cells are active and are synthesizing new protoplasm in
which stage of the growth in bacteria
a) Log phase
b) Lag phase
c) Stationary phase
d) None of these
3. The most active stage in the sigmoid curve of bacteria in which maximum
growth is attained
a) Lag phase
b) Stationary phase
c) Decline phase
d) Log phase
b) Exponential phase
c) Lag-phase
d) None
b) Generation time
c) Sigmoid curve
d) None of these
6. A culture of bacteria produces 5 generations in 2 hours. What is the
generation time for this bacterium under those conditions.
a) 15 minutes
b) 24 minutes
c) 30 minutes
d) 75 hours
b) Generation time
c) Sigmoid curve
d) None of these
b) Stationary phase
c) Lag phase
d) Log phase
9. If a single bacterium replicated every 30 minutes, how many bacteria would
be present in 2 hours?
a) 16
b) 32
c) 8
d) 4
c) changes in pH occur.
19/08/20
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Dept of Higher Education, Govt Of Gujarat
Dr Ajay Gor- Director Of HVPGR
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My Department, Staff Members and dear Students