Leukocyte

reduction/leukoreduction
Leukocyte reduction
• Is the removal of white blood cells(WBC) from blood components prior to transfusion
• In whole blood, red blood cells, Apheresis platelets is less than 5.0 x 106 residual white
blood cells and less than 8.3 x 105 residual white cells in per each platelets derived from
whole blood.
• Inaddition, at least 85% of the original component must be recovered after leukoreduction.
• Leukoreduction in of whole blood and blood components has shown to reduce recurrent
febrile non-hemolytic transfusion reactions, reduce alloimmunization to leukocyte antigens
that may complicate care of patients who undergo transplantation or chronic transfusion
therapy and protect against transmission of CMV disease
• Luekoreduction performed shortly after collection usually within 72 hours is considered
prestorage luekoreduction.
luekoreduction
• The impetus for prestorage leukoreduction involved biological
response modifiers (BRMs) released during storage of the component
that were found to promote febrile transfusion reaction. Example
BRMs include proinflamatory cytokines (I and interleukin-1,
interleukin-6, and tumor factor) and complement fragments(C5a and
C3a)
luekoreduction
• Leukorreduced RBCs and whole blood are prepared using filters that
achieve at least a 99.9% removal leukocytes by employing multiple
layers of polyester or cellulose acetate nonwoven fibers that trap
leukocytes and platelets but allow RBCs to flow through.