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EXE 6 - Group 5 - Phar 1-3 Haze
EXE 6 - Group 5 - Phar 1-3 Haze
EXE 6 - Group 5 - Phar 1-3 Haze
Products of Photosynthesis
Group 5:
Derije, Hershey
Equiza, Hazel
Genova, Erin
Genoves, Jago Jr.
OBJECTIVES
Photosynthesis
majorsupplier of organic compounds used in the synthesis of other
compounds (e.g. carbohydrates, proteins)
Oxidation
Releases stored energy to be utilized by the organism to carry out
essential metabolic processes.
Autotrophs
Provide the basic material and energy for all life
through photosynthesis.
Materials
Plant leaves Corn Microscope
Molasses Peanut Test tube holder
Cane sugar Cocoa Test tube rack
Raisin Coconut meat Test tubes with
Corn syrup Lugol’s solution stopper
Banana Fehling’s
Mortar and
solution pestle
Potato
Concentrated
Filter paper
Cassava
HNO3 Scissors and
Mongo seeds cutter
10% NaOH
Soya Droppers
Acetone
Water bath
Microscope
Procedures
Carbohydrates in plants
Fehling’s Reagent
Reminders and precautions
Make sure the opening of the test tube is away from you
or from your classmates.
Do not boil too vigorously or do not heat over an open
flame. When using a beaker as water bath, add boiling
chips in the water bath to avoid bumping.
Raisins
Fehling’s
Corn Syrup
Solution
Presence of sugar in a leaf extract
Fehling’s Reagent
Add about 3 mL of Fehling’s
Cut up a leaf which has been
solution (1.5 mL Fehling’s A
exposed to bright light and Filter the liquid into a test
and 1.5 mL of Fehling’s B)
crush it well with a little tube about 1/3 filled.
and then boil for a minute in
water.
a water bath
Fehling’s solution is an
indicator for the presence of
sugar.
Record the observed color of Record results in the
BLUE BRIGHT RED/BRICK
each tube. worksheet.
RED PRECIPITATE (AFTER
BOILING)
Presence of sugar
Presence of sugar in Molasses, Cane sugar, Raisin, and Corn Syrup
Fehling’s Reagent
In test tube A, put 1 mL of
molasses and 2 mL of water.
In test tube B, put a few Shake the materials well
Get 5 test tubes and label A,
crystals of cane sugar and 3 until the materials are
B, C, D, and E.
mL of water. In test tube C, thoroughly mixed.
mash a raisin and put in 3 mL
of water and in test tube D,
put 1 mL of corn syrup and 2
mL of water.
Lugol’s Solution
Reminders and precautions
Be cautious in cutting using a razor blade. Hold the sharp
edge using the cover. A scalpel blade with handle may be
used.
Potato
Cassava
Banana
Kamote Microscope
Lugol’s Solution
Presence of sugar in Molasses, Cane sugar, Raisin, and Corn Syrup
Lugol’s Solution
Make thin sections of the Mount in water in a slide and
following plant materials: examine with a low-power Draw and compare the starch
Banana, potato, cassava, and objective (LPO) under the grains.
kamote. microscope.
Xanthoproteic Test
Reminders and precautions
Concentrated nitric acid is a corrosive substance that produce
noxious gases. Avoid inhalation and contact with eyes and skin.
Wear appropriate PPE.
Make sure that the nitric acid that will be used is colorless. Do
not use it if it is yellow (or darker) in color.
DISPOSAL:
Dispose the residue together with the filter paper in the solid wastes
bin.
Alkalinify the solution by adding a slight excess of 10% sodium
bicarbonate. Allow the fizzing (effervescence) to stop. Dilute the
water and dispose in the sink with copious amount of water.
Xanthoproteic Test Materials
Litmus paper
Xanthoproteic test
Filter paper
Coconut meat
Cocoa powder
Fire
Vegetable oil
Presence of Proteins in Various Plant Materials
Chromatography
Reminders and precautions
Acetone is a volatile solvent, which is mildly irritant. Avoid
inhalation and contact with eyes and skin. Wear appropriate
PPE.
Place the test tube in a rack with minimal agitation. Do not
disturb while developing the chromatogram.
Leaf
Grass 85% Acetone