BLOOD DONOR

SCREENING
Arce Lubang
Bolima Mondoy
Gocela Penero
BMLS 4D
Republic Act No. 7799
• AN ACT PROMOTING VOLUNTARY BLOOD DONATION, PROVIDING
FOR AN ADEQUATE SUPPLY OF SAFE BLOOD, REGULATING BLOOD
BANKS, AND PROVIDING PENALTIES FOR VIOLATION THEREOF
• National Blood Services Act of 1994
• Every hospital in the PHL equipped with blood banking services
requires a standard protocol on screening blood donors
• Three Parts
I. Laboratory Screening
II. Medical History
III. Blood donation
LABORATORY SCREENING
Part I
 Materials Used in

Standard weighing scale Weight determination

Materials needed
BP apparatus: aneorid BP determination

Digital thermometer Temp determination

Blood lancet Hb determination

CuSO4 soln
Sg 1.053-1.055

Blood lancet Hct determination

Heparinized cappilet
Sealing clay
Paraffin wax
microhematocrit centrifuge
Microhematocrit reader

Blood lancet (3mm blade) Blood Type determination

Cotton
70% isopropyl alcohol
Typing sera (Anti-A, B, D)
Glass slides

Blood lancet (3mm blade) Tests for Blood Transmissible Dse

Cotton
70% isopropyl alcohol
Giemsa staining kit
Kits for HIV, HCV, HBsAg, RPR

Blood bag Bleeding demonstration

Tourniquet
Providone iodine
Squeeze ball
70% isopropyl alcohol
Spring balance
Blood Donor Interview Data Sheet
A. Weight determination
• Record the weight in pounds and kilograms
• 1 kg= 2.2 lbs
• The weight tells you the max amount of blood that can be withdrawn from a
donor
• Max allowable is suspended in 63 mL of anticoagulant
• If weight is <110 lbs/ <50 kg, collect lesser amount of blood and adjust
amount of anticoagulant and use blood within 24 hrs
BP determination
• Ask the donor what his previous BP reading was
in his last check up
• If the donor does not know or has no prior check up or BP reading, squeeze
bulb until the hands reaches 200mmHg
• Record the BP (systolic/ diastolic) in mmHg
• BP should be within allowable limits:
• Systolic p: ≤ 180 mmHg
• Diastolic p: ≤ 100 mmHg
• If initial BP is high and the donor has no history of being hypertensive, let the
donor rest for 15-10mins and repeat BP reading. If the BP is still high, reject
the donor and refer to a physician in the area.
Pulse determination

• Count the number of beats in exactly 1

minute by using your times
• Record in beats/ minute
• Acceptable pr: 50-100 bpm
• If initial pr is, let the donor rest for 15-10mins
and repeat pr reading. If the pr is still high,
reject the donor and refer to a physician in the
area.
Temperature determination
• Place the thermometer under armpit for 5 minutes
• Record temp in °C and °F
• °F = 1.8 °C + 32
• °C = (°F- 32)/ 1.8
• Acceptable temp:
• ≤37.5 °C or 99.5 °F
• High temp indicates active or ongoing infection. Donor must be referred
to a physician in the area
• Donors are advised not to have any coffee or hot beverages while
waiting
Hb determination
• CuSO4 Garvimetric Method
• If the drop of blood sinks within 15 seconds, Hb is estimated to be >
12.5 g/dL
• If the drop of blood floats or simply suspends, the Hb is estimated to
be <12.5 g/dL
• Record the Hb level as >12.5 g/dL or <12.5 g/dL
• Acceptable: ≥12.5 g/dL
Hct determination
• Use a heparinized blood and fill until ¾
• Seal one end with clay to permanently secure content with paraffin
wax and label properly.
• Spin for 5 minutes
• Record the Hct percentage
• Acceptable: ≥38% for allogeneic donation
• If less than acceptable, it may result to a deferral
Blood Type determination
• Use same puncture site from Hb and Hct determination
• Place drop of blood on a single glass slide divided into 3 portions
labeled A, B. and Rh
• Deliver Anti-A sera on labeled A, Anti-B on B and Anti-D on Rh
• Mix sample and antisera using different applicator sticks
• CHECKPOINT: If the donor fails this preliminary tests procedure,
he/she will not proceed to tests for blood transmissible diseases
Tests for Blood Transmissible Diseases
• Malarial Parasite Determination
• Prepare thin smear from skin puncture or EDTA blood from venipuncture
• Perform standard smear prep and staining using Giemsa stain
• Examine under OIO
• Report positive or negative
• Record results
Malarial Parasite
Tests for Blood Transmissible Diseases
• Serological Tests
• Perform venipuncture and place blood sample in a red top tube
• Allow blood to clot
• Centrifuge at 3400 rpm for 3 mins
• Separate serum and use for tests for presence of HIV, HCV, HbsAg and T.
pallidum infxn
• Report Reactive or Non-reactive
• Record results
BLOOD DONOR INTERVIEW
Part II
BLOOD DONATION
Part 3