Analyticon Biotechnologies AG

Lichtenfels - Germany

agile - affordable - accurate

1
 Basics of Hematology

Hemolyzer ®

agile - affordable - accurate

2
 Measuring Principles

 Laser light scatter technology for 5-part WBC differential

 Impedance measurement for WBC, RBC und PLT

 Light absorbance for measuring HGB

agile - affordable - accurate

3
 Types of blood cells

 RBC: 95% of all

blood cells

 PLT: ~4.8% of all

blood cells

 WBC: ~0.2% of all

blood cells
 NEU: 40-75%
 LYM: 20-45%
 MON: 2-8%
 EOS: 1-6%
 BAS: 0-1%

agile - affordable - accurate

4
 Differential Lysing Process

PLT & RBC LYM MON GRAN

RBCs WBCs selectively shrank to nuclei

destroyed
agile - affordable - accurate

5
 3-part Differential WBC Histogram
PLT & RBC LYM MON GRAN

& HGB

agile - affordable - accurate

6
 4-part Differential WBC Scattergram
Eosinophiles Neutrophiles
 Basophiles cannot
be reliably
differentiated from
the rest of the white
blood cells
 Additional optical
count is needed
Monocytes

Lymphocytes
Size

RBC GHOST Low angle forward scatter

agile - affordable - accurate

7
 Hemolyzer® 3

agile - affordable - accurate

8
 Hemolyzer® 3

 Provides 20 parameters CBC including 3-part WBC differential

 3 Histograms: WBC, RBC, PLT

 Throughput: 60 samples per hour

 6 QC lots, Levey-Jennings diagrams

 Database capacity: 10,000

 Only 3 reagents (cyanide-free)

 Automatic monitoring of reagent status

agile - affordable - accurate

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 Hemolyzer® 3

 Sample Volume: 25 μl (open tube sampling)

 Automatic clogging prevention

 Large, high resolution colour LCD

 Easy, intuitive operation

 Interfaces: RS232, USB

 Printout via internal thermal printer (optional

also via external printer)
 Compact design:
 350 x 490 x 385 mm
 Weight: 15 kg

agile - affordable - accurate

10
 Hemolyzer® 3: Maintenance

 Daily Maintenance
Hemolyzer Hypocleaner is inserted as sample and
“measured”

agile - affordable - accurate

11
 Hemolyzer® 3: Key Benefits

 Safe Operation
 The sample is being aspirated inside the analyzer,
practically excluding the risk of injuries for the user

 High speed

 Results within 60 seconds

 Reliable Instrument

 Easy maintenance by the user

agile - affordable - accurate

12
 Hemolyzer® 3: Competitors
Model Hemolyzer® 3 Cell-dyn® 1800 AC•T Diff AC•T Diff2 ABX Micros 60
Distributor Analyticon Abbott Beckman Coulter Beckman Coulter Horiba
           

WBC diff. 3 3 3 3 3
Number of parameters 20 18 18 18 18
Histograms WBC, RBC, PLT WBC, RBC, PLT WBC, RBC, PLT WBC, RBC, PLT WBC, RBC, PLT
Throughput (PT/h) 60 60 50 50 60 (open); 55(closed)
Sample volume [μl] 25 30 12 18 10

Open/Closed sampling /- /- /- / /

STAT Function     
Number of reagents 3 3 3 3 3
Bar code reader optional optional     optional
Autoloader (capacity) - - - - -
Colour display   - - -
Touch Screen - -   -
Internal printer  - - - 
RS-232/USB /  /-  /-  /-  /-
Database capacity 10,000 10,000 250 250 1; 78 (with smart card)
QC Levey-Jennings Levey-Jennings Levey-Jennings, IQAP Levey-Jennings  
Dimensions          
Size (WxDxH) [cm] 35x49x38.5 66x44x52 38x42x39 45x49x40 36x42x32

Weight [kg] 15 55 16 20 14
Operating temperature 15-35°C 18-30°C     16-34°C

agile - affordable - accurate

13
 Hemolyzer® 3: Competitors
Model Hemolyzer® 3 M 16M/C/20M/C M 16S/20S CA 620 KX 21
Distributor Analyticon Medonic Medonic Medonic Sysmex
           

WBC diff. 3 3 3 3 3
Number of parameters 20 16/20 16/20 9/16/20 18
Histograms WBC, RBC, PLT WBC, RBC, PLT WBC, RBC, PLT WBC, RBC, PLT WBC, RBC, PLT
Throughput (PT/h) 60 65 65 60 60
Sample volume [μl] 25 100 (open); 300 125 50
250 (closed)
Open/Closed sampling /- / (C only) / /optional /-
STAT Function     
Number of reagents 3 3 3 3 2
Bar code reader optional   optional optional
Autoloader (capacity) - -  (2x20) - -
Colour display    - -
Touch Screen -   - -
Internal printer  - - - 
RS-232/USB / / / /  /
Database capacity 10,000     220 300
QC Levey-Jennings Levey-Jennings Levey-Jennings Levey-Jennings Levey-Jennings
Dimensions          
Size (WxDxH) [cm] 35x49x38.5 29x41x46 33x43x46 42x35x46 42x48x36

Weight [kg] 15 18 22 22 28
Operating temperature 15-35°C 18-32°C 18-32°C 18-32°C  

agile - affordable - accurate

14
 Hemolyzer® 3: Competitors
Model Hemolyzer® 3 BC 3000+ BC 3200 BC 3600
Distributor Analyticon Mindray Mindray Mindray
         

WBC diff. 3 3 3 3
Number of parameters 20 19 19 21
Histograms WBC, RBC, PLT WBC, RBC, PLT WBC, RBC, PLT WBC, RBC, PLT
Throughput (PT/h) 60 60 60 60
Sample volume [μl] 25 13 13 20

Open/Closed sampling /- /- -/ /optional

STAT Function    
Number of reagents 3 3 5 3
Bar code reader optional optional optional optional
Autoloader (capacity) - - - -
Colour display    
Touch Screen - - - 
Internal printer    
RS-232/USB / / / /
Database capacity 10,000 35000 35000 40000
QC Levey-Jennings Levey-Jennings Levey-Jennings Levey-Jennings
Dimensions        
Size (WxDxH) [cm] 35x49x38.5 39x46x40 39x46x42 45x40x45

Weight [kg] 15 21 25 28
Operating temperature 15-35°C 15-30°C 15-30°C 15-30°C

agile - affordable - accurate

15
 Hemolyzer® 3: Competitors
Celltac α MEK- Mythic 18 H18 light Celly Plus
Model Hemolyzer® 3
6400/6410/6420
Distributor Analyticon Nihon Kohden Orphee SFRI Biocode Hycel
   

WBC diff. 3 3 3 3 3
Number of parameters 20 18 18 20 18
Histograms WBC, RBC, PLT WBC, RBC, PLT WBC, RBC, PLT WBC, RBC, PLT WBC, RBC, PLT
Throughput (PT/h) 60 63 60 60 60
Sample volume [μl] 25 30 9.8 9.6 10

Open/Closed sampling /- / /-    

STAT Function       
Number of reagents 3 4 3 3 3
Bar code reader optional optional optional   optional
Autoloader (capacity) - - - - -
Colour display   -  
Touch Screen -   - 
Internal printer  optional optional   
RS-232/USB / /- / / /
Database capacity 10,000 400 1,500 20,000 1,000
QC Levey-Jennings Levey-Jennings Levey-Jennings Levey-Jennings Levey-Jennings
Dimensions  
Size (WxDxH) [cm] 35x49x38.5 23x45x38.3 25x34x35 33x43x38 32x35.2x36.8

Weight [kg] 15   9 23 9.5

Operating temperature 15-35°C     18-35°C 18-35°C

agile - affordable - accurate

16
 Hemolyzer® 5

agile - affordable - accurate

17
 Hemolyzer® 5

 Provides 24 parameters CBC including 5-part WBC differential

 2 Histograms: RBC, PLT
 2 Scattergrams: 4-diff WBC, BASO

 Throughput: 60 samples per hour

 6 QC lots, Levey-Jennings diagrams

 Database capacity: 100,000

 Only 3 reagents (cyanide-free)

 Automatic monitoring of reagent status

agile - affordable - accurate

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 Hemolyzer® 5

 Sample Volume: 100 μl (open/closed, whole blood)

 Automatic clogging prevention

 Large colour LCD touch screen

 Easy, intuitive operation

 Autoloader (100 samples) optional

 Interfaces: RS232, USB

 Printout via external Microsoft®

WindowsTM compatible printers

agile - affordable - accurate

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 Hemolyzer® 5: Dimensions

 Compact design: 410 x 465 x 515 mm

 Weight: 35 kg (with autoloader: 49 kg)

agile - affordable - accurate

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 Hemolyzer® 5: Autoloader

 Plug-In; can be added any time

 Based on linear racks

 Up to 100 samples (10 racks)

 Cap recognition and cap piercing

 Monovette®, Vacutainer®, Vacuette®

 Integrated barcode reader

 Integrated sample mixer

 Using the same sample needle

agile - affordable - accurate

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 Hemolyzer® 5: 4-Diff Scattergram
Eosinophiles Neutrophiles

Monocytes

Lymphocytes
Size

RBC GHOST Low angle forward scatter

agile - affordable - accurate

22
 Hemolyzer® 5: Basophile Scattergram

 in the 4-part differential the basophiles cannot be reliably

differentiated from the rest of the white blood cells
 additional optical count is needed for basophiles

 in the WBC/HGB dilution all WBC’s are (over)lysed with the

exception of the basophiles
 after the impedance count the WBC/HGB dilution is transferred
to the optical head for BASO count

agile - affordable - accurate

23
 Hemolyzer® 5: Maintenance

 Daily Maintenance
Hemolyzer Hypocleaner is inserted as sample and
“measured”

Regular Maintenance (depending on throughput)

 Cleaning of measurement head

 Cleaning of shear valve

agile - affordable - accurate

24
 Hemolyzer® 5: Key Benefits

 Safe Operation
 The sample is being aspirated inside the analyzer,
practically excluding the risk of injuries for the user

 Patented Laser Technology

 High speed
 Results within 60 seconds

 Reliable Instrument
 Easy maintenance by the user

 Autoloader (100 samples)

 Upgrade can be done at any time

agile - affordable - accurate

25
 Hemolyzer® 5: Competitors
Model Hemolyzer® 5 Cell-dyn® 3200 CS Cell-dyn® 3200 AL Cell-dyn® 3700 CS Cell-dyn® 3700 SL
Distributor Analyticon Abbott Abbott Abbott Abbott
           
WBC diff. 5 5 5 5 5
Number of parameters 24 22 22 23 23
Histograms RBC, PLT RBC, PLT RBC, PLT RBC, PLT RBC, PLT
Scattergrams 4diff (L-M-N-E), BAS WBC, RBC, PLT, RET WBC, RBC, PLT, RET WBC, RBC, PLT, WBC, RBC, PLT,

Throughput (PT/h) 60 71 72 90 90
Sample volume [μl] 100 240 (closed); 150 (open) 240 (closed); 150 (open) 240 (closed); 130 (open) 355 (AL); 240 (closed);
130 (open)
Open/Closed sampling / / / / /
STAT Function         
Number of reagents 3 4 4 4 4
Bar code reader optional    
Autoloader (capacity) optional (100) -  (50) -  (100)
Standalone  - - - -
Touch Screen  - - - -
Internal printer - - - - -
RS-232/USB 1/4 /- /- /- /-
Database capacity 100,000 10,000 10,000 10,000 10,000
QC Levey-Jennings Levey-Jennings Levey-Jennings Levey-Jennings Levey-Jennings
Dimensions          
Size (WxDxH) [cm] 40x45x50 83x48x59 83x48x74 76x61x56 76x68x79

Weight [kg] 35 97 106 86 131

agile - affordable - accurate

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 Hemolyzer® 5: Competitors
Model Hemolyzer® 5 AC•TTM 5 Diff OV/CP AC•TTM 5 Diff AL HmX ABX Pentra (XL) 80
Distributor Analyticon Beckman Coulter Beckman Coulter Beckman Coulter Horiba
           
WBC diff. 5 5 5 5 5
Number of parameters 24 24 26 26 24
Histograms RBC, PLT RBC, PLT WBC, RBC, PLT WBC, RBC, PLT RBC, PLT
Scattergrams 4diff (L-M-N-E), BAS 4diff (L-M-N-E), BAS 4diff (L-M-N-E) 4diff (L-M-N-E) 

Throughput (PT/h) 60 60 60 80 75
Sample volume [μl] 100 53 (CBC + diff); 30 (CBC) 53 (CBC + diff); 30 (CBC) 185 (closed); 125 (open) 53 (CBC + diff); 30 (CBC)

Open/Closed sampling / / (CP) / / /

STAT Function      
Number of reagents 3 5 5 5 5
Bar code reader optional optional   
Autoloader (capacity) optional (100) -  (100)   (100)
Standalone   (OV)/-(CP) - - 
Touch Screen  - - - 
Internal printer - - - -  (laser)
RS-232/USB 1/4 / / / /2
Database capacity 100,000 10,000 10,000 5,000 10,000
QC Levey-Jennings IQAP IQAP IQAP Levey-Jennings
Dimensions          
Size (WxDxH) [cm] 40x45x50 44.4x58x50.1 81.5x58x54 61.5x61.7x84.8 82x57x54cm

Weight [kg] 35 36.2 55 94.5 55

agile - affordable - accurate

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 Hemolyzer® 5: Competitors
Model Hemolyzer® 5 BC 5300 BC 5380 BC 5500 BC 5800
Distributor Analyticon Mindray Mindray Mindray Mindray
           
WBC diff. 5 5 5 5 5
Number of parameters 24 27 27 27 29
Histograms RBC, PLT 3 3 RBC, PLT RBC, PLT
Scattergrams 4diff (L-M-N-E), BAS 1 1 4diff (L-M-N-E), BAS 4diff (L-M-N-E), BAS

Throughput (PT/h) 60 60 60 80 (AL); 60 (manual) 90

Sample volume [μl] 100 20 (CBC + diff); 15 (CBC) 20 (CBC + diff); 15 (CBC) 180 (closed); 120 (open) 180 (closed); 120 (open)

Open/Closed sampling / /- /- / /

STAT Function     
Number of reagents 3 5 5 6 6
Bar code reader optional optional  optional optional
Autoloader (capacity) optional (100) -  (30) optional (50) optional (50)
Standalone  - - - -
Touch Screen  - -  
Internal printer - - - - -
RS-232/USB 1/4     /4 /5
Database capacity 100,000 40,000 40,000 40,000 40,000
QC Levey-Jennings    
Dimensions          
Size (WxDxH) [cm] 40x45x50 41x53x47   66.4x61.3x58.5 66.4x61.3x58.6

Weight [kg] 35 45   73 76

agile - affordable - accurate

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 Hemolyzer® 5: Competitors
Model Hemolyzer® 5 XS 800i XS 1000i XT 1800i/2000i
Distributor Analyticon Sysmex Sysmex Sysmex
         
WBC diff. 5 5 5 5
Number of parameters 24 24 24 24/31
Histograms RBC, PLT RBC, PLT RBC, PLT RBC, PLT
Scattergrams 4diff (L-M-N-E), BAS   4diff, WBC/Baso, PLT,
Ret (2000i)
Throughput (PT/h) 60 60 60; AL: 52 80
Sample volume [μl] 100 20 20 85; 150 (AL)
Open/Closed sampling / /- / /
STAT Function    
Number of reagents 3 4 4 5
Bar code reader optional optional optional optional
Autoloader (capacity) optional (100) - optional (20) 
Standalone  - - -
Touch Screen  - - -
Internal printer - - - -
RS-232/USB 1/4 / /  
Database capacity 100,000 10,000 10,000 10,000
QC Levey-Jennings Levey-Jennings Levey-Jennings Levey-Jennings
Dimensions        
Size (WxDxH) [cm] 40x45x50 32x50x41 32x40x41 53x63x50

Weight [kg] 35 24 24 52

agile - affordable - accurate

29
 Hemolyzer® 5: Competitors
Model Hemolyzer® 5 Celltac E MEK-7222 Celltac E MEK-8222 Mythic 22 OT/CT Mythic 22 AL
Distributor Analyticon Nihon Kohden Nihon Kohden Orphee Orphee
           
WBC diff. 5 5 5 5 5
Number of parameters 24 22 22 22 22
Histograms RBC, PLT   RBC, PLT RBC, PLT
Scattergrams 4diff (L-M-N-E), BAS   5-diff 5-diff

Throughput (PT/h) 60 51 80 50(OT)/45(CT)

Sample volume [μl] 100 55 (CBC + diff); 30(CBC) 56 (CBC + diff); 30 (CBC) 17 (OT)/20 (CT) 20

Open/Closed sampling / /optional /optional / (CT only) /

STAT Function      
Number of reagents 3 5 5 3 3
Bar code reader optional optional optional optional 
Autoloader (capacity) optional (100) -  (50) -  (50)
Standalone     
Touch Screen     
Internal printer - optional optional - -
RS-232/USB 1/4 / / 1/2 1/5
Database capacity 100,000   100,000 1,500 1,500
QC Levey-Jennings Levey-Jennings Levey-Jennings Levey-Jennings Levey-Jennings
Dimensions  
Size (WxDxH) [cm] 40x45x50 61.3x58.3x55 25x34x35 48x40x40 38.2x48.5x52

Weight [kg] 35 55 11 25 38

agile - affordable - accurate

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 agile - affordable - accurate

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