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Microbiological Culture & Colony Count Microbiological Culture & Colony Count
Microbiological Culture & Colony Count Microbiological Culture & Colony Count
Microbiological Culture & Colony Count Microbiological Culture & Colony Count
Microbial growth
Temperature, PH,
Isotonic solution
Binary Fission
Bacterial colony
A visible mass of
microorganisms which
originating from a
single mother cell.
Microorganisms culture
Two basic culture techniques used:
70% ethanol
Sterile technique
Streak plate
Spread plate
Pour plate
Purpose of streaking
contaminants.
condensed moisture
Quadrant streaking for isolation pure culture
Streak plate procedure
quadrant
Streak on MAC & Nutrient agar
Spread plate method
• Generally 0.1ml of the diluted sample is pipetted
onto the surface of a solidified agar medium
Hold the bottle in the right hand; remove the cap with the little finger of the
left hand.
Lift the lid of the Petri dish slightly with the left hand and pour the
sterile molten agar into the Petri dish and replace the the lid.
Seal and incubate the plate in an inverted position at 37°C for 24-48 hours.
Pouring the molten agar and incubation
Flame the neck of the bottle and replace the cap
Gently rotate the dish to mix the culture and the medium
evenly.
1 ml 1 ml
1 ml 1 ml
Stock solution
9 ml diluent
25gm+225 ml 9 ml diluent 9 ml diluent
9 ml diluent
diluent
Stock solution
9 ml 9 ml
25gm+225 ml 9 ml 9 ml
diluent diluent
diluent diluent diluent
Dilution=Stock
volume/Total
volume 1/1+9=1/10= 1/1+9=1/10= 1/1+9=1/10= 1/1+9=1/10=
0.1=1x10-1 0.1=1x10-1 0.1=1x10-1 1x0.1=10-1
https://microbeonline.com/pour-plate-method-principle-
procedure-uses-dis-advantages