CHEMISTRY

INVESTIGATORY
PROJECT ON
CHALK
CHROMATOGRAPHY
SUBMITTED BY :-
Aditya Chauhan
ass –XII C ,Roll No.-3

SUBMITTED TO :-
MRS. Manju Kasana
PGT Chemistry
Greenway Modern School
 INDEX
S. No. TOPIC
1 CERTIFICATE
2 ACKNOWLEDGEMENT
3
 CERTIFICATE
This is to inform that ADITYA CHAUHAN of
class XII C studying in GREENWAY MODERN
SCHOOL has successfully Completed his project on
the topic CHALK CHROMATOGRAPHY under
my supervision and guidance as per the guidelines
issued by CBSE for the session 2020-2021

Mrs. Manju Kasana

(PGT Chemistry)

School Stamp

Teacher’s Signature
ACKNOWLEDGEMENT

I wish to express my sincere gratitude

and special thanks to my respected
Principal Mr. Mohit Sachdeva, my
Chemistry Teacher Mrs. Manju Kasana
and Laboratory Assistant Mrs. Devika
for their timely help and valueable
suggestion without which my
demonstration would not have been
successful.
 AIM

To separate the color

components present in
the sketch pen ink (given
below) by ascending chalk
chromatography and find
their Rf value
 Material Required
• Sketchpen Ink
(Green,Brown,Black)
• Beaker
• Water
• Three Chalks
• Ruler
• Pencil
 THEORY
 In Chalk Chromatography, there is a stationary
phase which is the dusty Chalk and the mobile
phase which is a liquid solvent (or mixture of
solvents) used to carry the sample solutes under
analysis along the chalk. Usually, one uses
chromatography to find out the components of a
sample which are separated depending how
much soluble these are in particular solvents and
hence how far they travel along the chalk. In
order to make the technique more scientific
rather than a mere interpretation by sight, the
Retention Value (Rf value for short) was applied
in chromatography. A particular compound  will
travel the same distance along the stationary
phase by a specific solvent (or solvent mixture)
given that other experimental conditions are kept
constant
 The Rf value is defined as the ratio of
the distance moved by the solute (i.e.
the dye or pigment under test) and
the distance moved by the solvent
(known as the Solvent front) along
the chalk, where both distances are
measured from the common Origin
or Application Baseline, that is the
point where the sample is initially
spotted on the paper. Distance from
Baseline travelled by Solute Distance from
Baseline travelled by Solvent

Rf value= Distance from baseline travelled by solute

Distance from baseline travelled by solvent
 PROCEDURE
1. Take a chalk of approximately 7 cm.
2. Draw a horizontal line from pencil at distance of 1
cm from the base of the chalk. Mark a point with
pencil on the line drawn.
3. Spot the color ink directly with a sketch pen at the
point marked before. Care should be taken that the
spot is small in size. Repeated spotting may be done
to enrich the spot, to increase the concentration of
component.
4. Take a clean beaker and fill it with 1-2 ml of water.
There should be no droplets sticking inside the walls
of beaker.
5. Insert the chalk carefully in the beaker so that only
the spot on the chalk remains above the solvent
(water).
6. Keep the beaker in a safe place and leave it
undisturbed.
7. Notice the rise of the solvent and the components
in the upward direction
8. After sometime, the spot will
separate into different colors and the
solvent moves ahead of the colors.
9. Remove the chalk from the beaker,
Dry the chalk and mark the levels of
the solvent and color spots from the
horizontal line drawn before.
10. Record the observation in the
tabular form.
11. Calculate the Rf value for various
components