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Microtomy and H&E Stain
Microtomy and H&E Stain
Microtomy and H&E Stain
H&E stain
by Honorable GAKWAYA
1. Microtomy and paraffin
section preparation
Sectioning of tissue require some skills and
experiences in histotechniques
Alcohol
Mainly ethanol, denatured ethanol,
isopropyl alcohol.
Alcohol can dehydrate or hydrate
tissue
Reagent con’t
Hematoxylin
• This is for deparaffinize, hydrate tissue and
stains the chromatin of cell nucleus.
Bluing Reagent
• It change hematoxylin reddish color to blue
Reagent con’t