Institute of Technology of Cambodia

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Institute of Technology of Cambodia

Faculty of Chemical and Food Engineering

Development of gluten free breads from Colocasia esculenta


flour blended with hydrocolloids and enzymes
Lecturer : MOM Vattana
Group: I4-A1
Ms. BORITH Sievmorkyen Ms. HENG Soklay
Ms. CHHAY Phalla Mr. KEO Songlay
Mr. CHHOUN Theara Mr. KHUN Lavil
Ms. DARA Dalis

Academic Year: 2019-2020


Content

I INTRODUCTION

II OBJECTIVE

III METHODOLOGY

IV RESULT and DISCUSSION

V CONCLUSION
Introduction

Colocasia esculefrom is Taro which is a


plant root where origin South-east Asia
which rich in Carbohydrates source and
contain gluten free (GF).

Gluten is protein which is not good


to people who is celiac disease

1
Objective

 Identify nutritional value of


Colocasia esculenta flour

 Comparation the quality of different


formulation of Colocasia esculenta
bread making .

2
Material and Method Gluten free bread recipes

Ingredients (g) F1 F2 F3 F4 F5 F6 F7 F8

Flour 100 100 100 100 100 100 100 80


Water 227 227 227 227 227 227 227 227
Salt 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5
Compressed yeast 3 3 3 3 3 3 3 3
Sugar 2 2 2 2 2 2 2 2
Oil 2 2 2 2 2 2 2 2
HPCM 2 0.29
Xanthan gum 0.21
Guar gum 0.50
Gluzyme Mono 0.01
Izyme BA 0.1
Alcalase 1.5MG 0.01
Potato starch 20
3
Materials and Method

FLOUR CHARACTERISTICS:
FLOUR CHARACTERISTICS: STANDARD
STANDARD
METHOD(AACC,1999,AOAC,1990)
METHOD(AACC,1999,AOAC,1990)

Moisture: Oven
Moisture: Oven drying
drying (AACCI
(AACCI method
method 44-15.02)
44-15.02) Oven drying Muffle
Total nitrogen
Total nitrogen content:
content: Kjeldahl
Kjeldahl ,AACCI
,AACCI method
method
44-12.01
44-12.01
Fat content:
Fat content: Soxhlet
Soxhlet (AACCMethod
(AACCMethod 30-25.01)
30-25.01)

Ash content:
Ash content: Muffle
Muffle (AACC
(AACC method
method 08-01.01)
08-01.01)

Crude fiber:
Crude fiber: AOAC
AOAC method
method 973.18
973.18 Kjelflex k- Soxtherm
360 analyzer
Water binding
Water binding capacity:
capacity: Cornejo
Cornejo and
and rosell(2015)
rosell(2015)

Fibertherm FT12 4
Materials and Method
Quality assessment of GF breads Hardness (g)
Cohesiveness

Bread moisture content: AACC (1999) Chewiness (g)


Texture Parameter
Resilience
Springiness

Specific volume: Rapeseed


displacement method Crumb color

Bread volume: Ratio between the


volume of bread and its weight

Minolta colorimeter
Weight loss during baking: Weighing
the pan before and after baking Texture Analyzer TA-XT2i 5
Materials and Method
In vitro digestion
Digestible of GF
bread

  Equation Kinetic: C = C)

Starch:RSD,SDS,RS Glucose (mg) × 0.9

Hydrolysis index ( HI ): Calculate as the


ratio area under hydrolysis curve ( 0-
180 min )

Glucose
Expected glycemic index ( eGI ): 8.198+
0.862HI

glucose oxidase–peroxidase (GOPOD) kit 6


Result and Discussion

NUTRITIONAL VALUE OF COLOCASIA ESCULENTS


80 75.44
CORMELS FLOUR
70

60

50
Percentages %

40

30

20

8.28
10 5.04 6.33
4.38
0.53
0
Carb Protein Ash Fat Crude fiber Moisture

Parameters

7
Result and Discussion
62 Moisture of Bread Weigh loss of bread
18
60.96 16.3
61
60.23 16 15.49 14.93
60 59.69 13.98
59.3 13.8 13.89
14 13.07
58.8 12.78
Moisture value (g/100g)

59 58.74

12
58 57.63

Percentages %
10
57

56 55.56 8

55 6

54 4

53
2
52
F1 F2 F3 F4 F5 F6 F7 F8 0
F1 F2 F3 F4 F5 F6 F7 F8
formulation
formulation

8
Result and Discussion
70
Color of Bread L* a* b*

12 Specific volume of Bread


60
57.01 57.48 56.87 57.21 57.41
2.71 56.09 55.02 53.9
10
50
Specific volume (ml/g)

8
40
1.74 1.7
1.63 1.67 1.65

Color
6
30
1.2 23.97
1.11 22.29
21.04 21.75 20.7 20.74
20.34
4 20 18.43

2 10 6.88 7.09
5.18 6.13 5.85 5.92 5.63 4.63

0 0
F1 F2 F3 F4 F5 F6 F7 F1 F2 F3 F4 F5 F6 F7 F8

Formulation Formulation

9
Result and Discussion

Hardness of Bread Cohesiveness of Bread

400 0.6
361
350 330 0.5
316 323
0.5
300
263 0.38
0.4 0.37
250 233 0.34 0.35
0.33 0.33
Hardness (g)

Cohesiveness
209 0.31
191
200 0.3

150
0.2
100
0.1
50

0 0
F1 F2 F3 F4 F5 F6 F7 F8 F1 F2 F3 F4 F5 F6 F7 F8

Formulation Formulation

10
Result and Discussion

Chewiness of Bread

80

69
70 65

60
54
50
50 45
Chewiness (g)

39
40

30 27
24

20

10

0
F1 F2 F3 F4 F5 F6 F7 F8

11
Formulation
Result and Discussion
Springiness of Bread
0.8
Resilience of Bread
0.7
0.7
0.16
0.14
0.14 0.6
0.13 0.55
0.12 0.48
0.5 0.47 0.47
0.1 0.1 0.1 0.44 0.43
0.1 0.42

Springiness
0.1
0.09
0.4
Resilience

0.08

0.3
0.06

0.04 0.2

0.02
0.1
0
F1 F2 F3 F5 F6 F7 F8 0
F1 F2 F3 F4 F5 F6 F7 F8
Type of bread Formulation
Type of bread Formulation

12
Result and Discussion
Digestibility of starch (%)

14

Digestibility of GF breads RDS SDS RS


12.36
12

10

8 7.31 7.29
7.22 7.29
7.01 7.17 7.2
6.89
6.73 6.49
6.03 6.23 6.05
5.95 5.8 5.86
6 5.63
5.32 5.25 5.15
4.98
4.38
3.81
4

0
F1 F2 F3 F4 F5 F6 F7 F8

Type of bread formulation

13
Result and Discussion
Kinetic Constant Maximum Hydrolysis

H y d r o ly s is
K in etic Con stan t

0.06

M a x im u m
0.05
0.05 18 16.66 16.24
15.82 16.1
16
0.04 13.87
13.32 13.56
14
12.25
0.03 0.02 0.02 12

0.02 0.02 10
0.02 0.02
0.01 0.01 8
0.01 6
4
0
F1 F2 F3 F4 F5 F6 F7 F8 2
0
Type of formulated bread F1 F2 F3 F4 F5 F6 F7 F8

Type of formulated bread

14
Result and Discussion
A r e a u n d e r H y d r o ly s is c u r v e

 ACU  HI

30 28.55

3000 25
2576
20.89 21.02 21.49
2500 19.01 18.21
20 17.35 17.29
1885 1897 1939
2000
1715 1643

HI (%)
1565 1560 15
1500

10
1000

5
500

0 0
F1 F2 F3 F4 F5 F6 F7 F8 F1 F2 F3 F4 F5 F6 F7 F8

Type of bread formulation


Type of bread formulation

15
Result and Discussion
 eGI

eGI
35

30
%eGIe

25

20

15

10

0
F1 F2 F3 F4 F5 F6 F7 F8

16
Type of bread formulation
Result and Discussion
In vitro and expected glycemic index of GF breads

17
Conclusion

 Nutritionals Value

 Volume

 Technological quality

Light bread crumb structure

18
Thank you for
attention!
21

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