Microbiology

Laboratoric Test
Specimen collection for Ziehl-
Neelsen Staining

Desinfeksi kulit dengan alkohol 70 %

Specimen collection for Ziehl-
Neelsen Staining

Tekan cuping telinga dengan menggunakan dua jari sampai pucat

Specimen collection for Ziehl-
Neelsen Staining

Sayat dengan menggunakan blade scalpel No. 15

Specimen collection for Ziehl-
Neelsen Staining

Putar blade scalpel 90o

Specimen collection for Ziehl-
Neelsen Staining

Kumpulkan bubur jaringan, jika ada darah prosedur diulangi lagi

Ziehl-Neelsen Staining
• Material
• Ziehl’s carbolfuchsin
prepared with either • microscope
Tergitol No. 4 (a drop per
• bibulous paper
30 ml of carbolfuchsin) or
Triton-X (2 drops per 100 • paper toweling
ml of carbolfuchsin). • lens paper and lens cleaner
• alkaline methylene blue • immersion oil
• acid-alcohol • staining racks
• clean glass slides • 1-ml pipettes with pipettor
• inoculating loop • Hand gloove
• hot plate
Ziehl-Neelsen Staining
Ziehl-Neelsen Staining
Ziehl-Neelsen Staining
Ziehl-Neelsen Staining
Ziehl-Neelsen Staining
Ziehl-Neelsen Staining
Ziehl-Neelsen Staining
Ziehl-Neelsen Staining

Examination the preparation under

microscope ( x100) for confirmation
Microscopic result for Ziehl-
Neelsen Staining

Acid Fast Bacteria