的迈瑞 A GLOBAL PARTNER YOU CAN

Trouble Shooting

Diego Fang
International Customer Service Dept.
Mindray Co., Ltd.
1 Trouble Shooting Flow
Yes

Visible Trouble Record error Locate the source with

No
Customers (Mechanical stopping/noise messages on the the instruction of Service
complaints
/liquid leakage Error Log report Manual
/bad smell)

Yes
Have
spare
parts?

No
No

Repair or replace Ask for RMA

End Tes number from
defective parts
Yes t mindray
OK
?

Get new parts Return defective

parts
2 Error Description
2.1 Error Code Classification
 50000-XX-XX: PC software error
 10064-XX-XX: Main unit error
 10068-XX-XX: Sample unit error
 10069-XX-XX: Reagent unit error
 10067-XX-XX: Mixing unit error
 10066-XX-XX: Temperature control unit error
 10065-XX-XX:
Reaction tray and photoelectric unit error
 Other: Alarm information
2.2 Error Level

 Warning
 Pause
 Stop
 Emergency stop
 Test forbidden
 Startup refusal
2.3 Error Code List

 Refer to Chapter 6 of Service Manual

3 Judging Errors
on the Reaction Curve
3.1 Reaction Curve

 The BS-200 analyzer features in its reaction

curve. The curve and reaction data shows the
whole reaction process to engineers and
customers, which enables users to find and
solve problems more easily. Causes for
almost all result-related errors can be found
on the reaction curve.
 3.2 Example of Reaction Curve
(GGT)

F
C E
A
B
0

D
 3.3 Description
 As shown in the reaction curve above, the vertical axis indicates
the absorbance (real value = value in this curve/10000).The
intervals on the vertical axis of the reaction curve is not fixed.
 The horizontal axis indicates the measuring cycle, which is 16
seconds for the BS-200 analyzer.
 A normal double-reagent reaction curve is usually divided into 6
phases:
 A: time when R1 is added;
 B: time when R1 is preheated;
 C: time when sample is added;
 D: incubation time of sample and R1;
 E: time when R2 is added;
 F: reaction time.
3.4 Phase Description
 Phase A: Time When R1 Is Added

The point 0 indicates the cuvette blank

(which is considered as the referenced
absorbance for calculating ). In other
words,calculating absorbance = real
0:cuvette blank
absorbance – cuvette blank.

0
Phase A: Time When R1 Is Added

In the first cycle, R1 is added into the

reaction cuvette, so the absorbance
value here is the reagent blank of R1.
Phase A: Time When R1 Is Added

A:Usually it ranges from 12000

to 20000 (R1 of ALT)

Absorbance values at Point A vary with different reagents. If

the absorbance is much smaller than the value specified in
the reagent instructions, the reagent may have lapsed. On
the other hand, if the absorbance at Point A exceeds 50000
(except for reagent for CO2), visually check whether there is
any contamination or bubble in the reaction cuvette.
Phase B: Time When R1 Is Preheated

B:Usually, absorbance
does not change when
the reagent is heated.
Phase B: Time When R1 Is Preheated

Absorbance values of some reagents

may have a increase/decrease of
about 200 (0.02A).
Phase B: Potential Problems

 The normal fluctuation of absorbance

between two continuous cycles is no more
than 50. Otherwise there may be problems
as below:
•Bubbles or contamination in cuvette. In this case,
the absorbance fluctuates in some tests, but not all
tests.
•The photoelectric conversion time matches the
optical path not well. Observed in all tests for a
specific wavelength.
•The optical path is not stable such as an aged
lamp.
Phase C: Time When Sample Is Added

Showing the sample is

added into the reaction
cuvette.
Phase C: Potential Problems
 Possible causes for
inconsistent absorbance
values:
 Liquid dropping from
probe;
 level detection failure for
sample; In the same reduplicate
 fluid tubing leakage; tests,different sample
 malfunction of the mixing
bar;
volume makes different
 over high position of the absorbance on point C.
mixing bar;
 unfixed position of the
sample syringe.
Phase D: Incubation Time

IfThe reactionofcurve
fluctuation in this exceeds
absorbance
50phase is supposed
between to be cycles,
two continuous
smooth
check or changing
whether smoothly.
the mixing bar strikes
the cuvette.
 Phase E: Time When R2 Is Added
 Similar situation as on point A and point C.
 Point E is also considered as the starting time of the reaction in
the software.
 The possible problems here may be:
 Failure of adding R2;

 Bubbles or contamination with R2;

 Mixing bar strikes the cuvette.

Phase F: Reaction Time

Kinetic
Endpoint Decline
Incline
Phase F: Potential Problems
Most problems here will appear somewhere on the same reaction
curve, and the causes are also similar. Such as the fluctuation
caused by mixing bar ,or insufficient response as a result of the
LLD failure.

The impact of R2 may be considered

when there is nothing abnormal on
the other parts of the reaction curve.
3.5 Reaction Curve Review
 Even all the problems of the instruments and the reagents will
result in an abnormal reaction curve. But some abnormal
reaction curves seem normal.
 Different reaction type, different
curve shape. So a nearly
invisible fluctuation here may
impact other tests much. Watch
the instruction of the reagent
carefully.
How to know?
 Repeat the tests is very
important. It can help you find
the difference of the reaction
curves. That is where the
problems are.
4 Abnormal Test Result

 All results abnormal;

 Item results abnormal;
 Accidental results abnormal.

Application of reaction curve!

 4.1 All Results Abnormal
 Symptoms:
 All results are low;
 Some are high, and some are low, resulting in bad
repeatability.
 Practice:All Results Low
 How do you think about the reaction curve like if
there are:

 The added sample and reagent is

insufficient (due to blocked probe,
fluid tubing leakage and/or syringe
leakage);

 The reaction is not thorough enough (because the mixing

bar is too high, and/or the mixing bar does not work).
 Practice:Some High,Some Low
• How do you think about the reaction curve like if :

 The mixing bar strikes the cuvette;

 Reagent usually has bubbles;

 The lamp is aged, the filter is aged, the

photometric system is dirty, and/or the
photoelectric conversion position is incorrect.
4.2 Item Results Abnormal
In case of such an error, check the
repeatability of the test.

Find the rules on the reaction

curves.

 The specific reagent deteriorated;

 Optical path problems of the specific wavelength;
 Photoelectric conversion position problems of the
specific wavelength;
 Wrong calibration.
4.3 Accidental Results Abnormal
 If the results are normal
in the repeated tests, the
possible cause is dirty
reaction cuvette or
bubbles aspirated into
the reagent probe.
 If the results are still
abnormal, the possible
cause exist in the
sample.
5 Liquid Level Detection(LLD) Error

 There are three LLD errors:

 LLD error in reagent disc;

 LLD error in sample disc;
 LLD error in washing pool.
 5.1 LLD Error: Reagent liquid level
 The probe fails to detect the reagent liquid level :
A) Liquid level detection signal error
Symptom: The detection indicator is off or always on.
Abnormal assembly: Main control board or connection cable (The
12V working voltage is unavailable); reagent probe(broken or over high
position); disabled LLD board.
B) Transmission error
Symptom: There is an alarm even when the detection indicator is on.
Abnormal assembly: Probe connection cable is disconnected or in ill
contact.
C) Processing error
Symptom: The indicator is on, and the connection cable is enabled.
Abnormal assembly: Main control board.
 5.2 LLD Error: Sample level
 Two symptoms :
A:failing to detect liquid level in sample tray;
B:failing to detect liquid level in reaction tray.

The same causes as

Both A and that of the reagent
B: part(chapter 5.1)
No/insufficient The probe mistakes to
Only B: reagent in the detect the liquid level.
cuvette
 5.3 LLD Error of The Probe in Washing
Pool

 Two symptoms : No water in washing pool; water

available in washing pool.
 No water in washing pool
The distill water container is empty (the sensor is disabled);
The exterior-washing pump is disabled (no water in all pools);
The tubing is blocked (single-way valve blocked, 3-way connector
blocked);
The tubing is disconnected.
 Water available in washing pool
The same as that of the probe failing to detect liquid level in the
reagent part.
5.4 LLD Error Caused by Little Tubes
 The capacity of liquid
aspirated only depend
on the depth of the
probe under the liquid
level.
 Different area of cross
section, different
capacity.
 Using little tube instead
of standard tube will
cause insufficient sample
/reagent.
 6 Liquid Dropping From Probes

• Three possible causes:

 Fluid tubing leakage
(syringe/connector/valve)
 LLD error
 Other problems (dirty probes/unqualified
probe driver assembly and so on)
Cause1: Fluid Tubing Leakage
 Distinct symptoms:
In this case, the liquid drop in a large scale, some
of which are relatively big, and dropping happens
even when the probes are still.
 Solution:
Check the syringe piston/tubing connector/valves
on the leaking side and repair/replace the
defective ones.
 Cause2: LLD Error
 Distinct symptoms:
In this case, most drops are found between the reaction
tray and the washing pool.
 Solution:
 Correct the over high position of the rocker-arm;

 Be sure there is no bubble in the reagent bottle and the

cuvette;
 Be sure that the probe is in the center while dispensing

liquid;
 Tight the screw for fixing the syringe .

Note:The probe will NOT detect liquid level in the

reaction tray when add reagent.
 Cause3: Other Problems

A) Distorted probe tip

Cause: The probe tip is distorted, resulting in carryover.
Solution: Replace the probe.
B) Dirty exterior of the probe
Cause: Contaminated by samples, resulting in carryover.
Solution: Clean or replace the probe.
C) Unqualified probe driver assemblies
Cause: The probe driver assemblies are unqualified,
resulting in severe jitters at the moment when the probe
is rising.
Solution: Replace the defective ones.
 7 Problems in Photoelectric Unit
Insufficient Light Intensity:
A) Optical path
Symptom: Low background of several wavelengths,
specially of the short wavelengths.
Solution: Clean the end face of the optical fiber. And
increase the gain if it is too low yet.
B) Aged lamp
Symptom: Either of the background of the 340nm
wavelength or reference wavelength is lower than 43000.
Solution: Replace the lamp.
 Insufficient Light Intensity 2
C) Aged filter
Symptom: After the lamp is replaced, the
background of a certain wavelength is still very low.
Solution: Replace the defective pre-amplification
assembly.
D) Defective pre-amplification board
Symptom: The background of a certain wavelength
is nearly 0.
Solution: Replace the defective pre-amplification
board.
Photoelectric Conversion Position
Match Not Well

B: Indicate the time of

A/D converting. Cuvette center

Air between
A: Photoelectric signal Cuvette wall cuvettes
of a certain wavelength.
 8 Temperature Control Error

 Only the reagent preheating has an alarm.

Causes:
 The preheating temperature transducer is disconnected;
 The preheating temperature transducer does not work;
 The preheating system is switched off.
 Both the reagent preheating and reaction tray have
alarms.
Causes:
 Temperature control parameters are lost.
Solution for This Error
 Monitoring the temperature curve;
 Correct the temperature control
parameters using the Parameter
Configuration List;
 Measuring the impedance of the
temperature transducer, and replace
the disabled one;
 Replace the heater.
 Summary
 Here we can only give you a suggestion on
how to find the problems. Actually the
problems you facing always result in
multiform symptoms. Do observe the reaction
curve carefully, and find out the roots of the
errors. Most alarm information of the BS-200
analyzer is obtained based on sensor signals,
so it is necessary to learn the signal flows.
Note: Backup the important data and parameters
when you prepare to replace some parts.
Thanks!