Harvest Effectivity and Effect of Salinity on

Lipid Content Euglena sp. with

Scenedesmus sp. as a Bioflocculant

ALVINA SETIAWARDANI 18/426431/BI/10023

SUPERVISOR: DR. EKO AGUS SUYONO, S.SI., M. APP.SC

FACULTY OF BIOLOGY
GADJAH MADA UNIVERSITY
YOGYAKARTA
2021
Background
Based on the Energy
Information Agency (EIA)
report, it can be estimated
that by 2030 the world
will need 60% more
energy than it is now
(Ahmad et al., 2011).
Euglena sp. have the
ability to do a higher
nutrient removal and a
faster growth rate than
other types of microalgae
(Inwongwan et al., 2019).
It is known that some
microalgae species contain
lipids excess up to 70% and
can grow exponentially under
optimal conditions (Amir &
Singh, 2018; Bahadar & Bilal
Khan, 2013).
Estimately,
Scenedesmus sp.
contain lipids in a
range of 19,6% to
21,1% (Elystia et al.,
2019).
HOW?
HOW?
HOW?
High lipid content which
is owned by this
microalgae will be the
ideal raw material for
renewable fuels
(Jegathese & Farid, LARGER CLUMPS?
2014). MORE LIPIDS?
Flocculation is a process carried out
for particles suspended can clump to
form larger clumps due to induction
(Agunbiade et al., 2018).
 Literature Review 1 Euglena sp. 2 Scenedesmus sp.

Euglena sp. it can even accumulate a

number of spare paramilon
polysaccharides, β-1,3-glucan which
makes up more than 80% of the dry
weight which is stored in granule
form in the cytoplasm. Biomass
contained in Euglena sp. are essential
amino acids, vitamins, lipids,
Especially Euglena gracilis which is a very
carbohydrates, and proteins (Gissibl
promising species can also produce various
et al., 2019). Scenedesmus sp. has the ability to achieve high
products such as wax esters, paramylon (β-
levels of carbon dioxide fixation even in
1,3-glucans), β-carotene, vitamin C and
environmental conditions with low carbon
vitamin E. (Toyama et al., 2019)
dioxide content. Scenedesmus sp. has been
shown to accumulate intracellular lipids. This is
the reason for the cultivation of Scenedesmus sp.
is an alternative solution for sustainable
renewable energy so that it can be used for the
Microalgae contain polyunsaturated fatty acids commercial industry (Mercado et al., 2020).
(PUFA) and saturated fatty acids such as
3 Fatty Acids
myristic acid and palmitic acid, or
monounsaturated fatty acids which are often
called MUFAs such as oleic acid (Roy & Pal,
2015).
Research’s Problems & Aims

Problems 1. How does the effect of adding Scenedesmus sp. as

microalga flocculants against non-flocculant microalgae
Euglena sp.?
2. How does the variation of salinity affect lipid
production and rate growth of nonfloculant microalgae
Euglena sp.?

Aims 1. Understand the effect of adding Scenedesmus sp. as

microalgae flocculants against non-flocculant microalgae
Euglena sp.
2. Understand the effect of variations in salinity on lipid
production and rate growth of Euglena sp
Research’s Benefit & Hypotheses

Research’s Benefit 1. Effect of adding Scenedesmus sp. as a

flocculant microalgae against non-
flocculant microalgae Euglena sp. can be
known.
2. Effect of salinity variations on lipid
production and growth rate of Euglena sp.
can be known.

1. The difference in salinity affects the lipid Hypotheses

content and growth rated culture
Euglena sp.
2. The addition of the flocculant microalgae
Scenedesmus sp. effect on the percentage of
precipitation and lipid content of
Euglena sp.
 Materials
Equipments

Tabel. 1. Cramer & Myers Medium Composition

Materials used in this

research are :
Conical Tube Micropipette Autoclave Centrifuge

• Cramers and
Myers (CM)
Medium 24 & 96 Well Spektrophotometer Vortex Erlenmeyer
Plate
• Samples of
Euglena sp &
Scenedesmus sp.
• Methanol &
Oven Aerator Kuvet Labu Ukur
Kloroform for
Bligh & Dyer
Analysis

Microscope Microscope LAF Cawan Petri

Binokuler Inverted
 Method
1 2 Cultivation of Euglena sp. 3 Cell density
Medium Preparation : & Scenedesmus sp. : Culture measurement using a
CM medium is stock put in a culture bottle hemocytometer.
composed of reagent with CM medium and Samples were taken
and carbon sources homogenized. On Euglena sp. every day for 14 days
given variation of salinity 2 with 3 repetitions.
ppt, 11 ppt, and 28 ppt.

6 5 4
7

Lipid content Dry Weight

Data Analysis using Biofloculation with a ratio of
Measurement using Measurement using
Microsoft Excel, 1:0.25 ; 1:0.5 ; 1:1 and do 3
the modified Bligh & the gravimetric
ANOVA, SPSS. repetitions.
Dyer (1959) method. method.
1 2 3
0,25
1 1 0.5 1 1
A B A B A B
Figure 1. simulation of the treatment of Euglena sp. and Scenedesmus sp.

A : Flocculant Microalgae (Euglena sp.)

B : Nonflocculant Microalgae (Scenedesmus sp.)

Tabel 2.Treatment of Salinity Levels

(ppt) (gr/L)
2 ppt 2 (gr/L)
11 ppt 11 (gr/L)
28 ppt 28 (gr/L)

NOTE
NOTE :: 11 ppt
ppt (part
(part per
perthousand)
thousand) == 1.000
1.000 ppm
ppm (part
(part per
permillion)
million)
11 ppm
ppm == 11 mg/L
mg/L
11 gram
gram == 1.000mg
1.000mg
 References
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