Produksi Biomassa

Dari Limbah
Dr. Megga Ratnasari Pikoli, M.Si.

Anggota:
• Andi Alwi A (11180950000106)
• Aulia Dinyati L (11180950000108)
• Inneztia A (11180950000110)
• M. Farhan H (11180950000111)
 TABLE OF CONTENTS

1 INTRODUCTION

2 METHODE

3 RESULT AND
DISCUSSION
4 CONCLUSION
INTRODUCTION
Oyster mushroom is generally appreciated for
its flavour and has been widely studied due to
its nutritional and medicinal proprieties. Oyster
mushroom can be grown in various agro-
industrial wastes, such as crop waste, soybean
straw and cotton stalks due to its extensive
enzyme systems which are capable of
degrading complex organic compounds in
biological wastes.
 Tea products, such as instant tea powder
and tea polyphenols, produced by
TEA WASTE extracting water-soluble components from
tea leaves, have gained vast popularity.
However, after extracting water-soluble
components from tea leaves a massive
amount of tea waste is left.
 Selecting an economic and efficient substrate material to reduce
production costs has been an important consideration in mushroom
cultivation.

Cottonseed hull is widely used as the main raw material in the

production of oyster mushroom, but the price is gradually rising.

Compared with cottonseed hull, tea waste is now treated as a waste

material with little value.

The objective of this research was to investigate whether tea waste

could be used as a substitute substrate for cottonseed hull in
cultivating different strains of oyster mushroom.
METHOD
● Tea waste
● Cottonseed hull
● Three mushroom strains (39,71 an
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LINDA ROE
 Preparation of cultivation substrate

• Each treatment contained 300 g dry substrate

• Tap water was added into each mixed dry substrate to adjust the
moisture to be about 65% (w/w).

• The pH was adjusted to 7–7.5 by adding gypsum to each substrat

• Polypropylene bags (35 × 17 cm2) were well filled with the

prepared substrates so that they were tightly packed, then securely
closed with plastic ties, and sterilised at 121 ∘C for 1 h. After
cooling, each bag was spawned with 8% (w/w) mushroom
mycelia grown on wheat grains on a dry weight basis of substrate.
Mushroom cultivation and harvesting

1. for spawning, the temperature was 25±2 ∘C, humidity was 50–60%, and and all bags
were placed in darkness (light intensity.

2. for fruiting body formation, the temperature was 23±2 ∘C, humidity was 80–90%, and
the illumination was 150–200 lux

3. Mushrooms were harvested when the mushroom cap surface was flat to slightly up-
rolled at the cap margins.

4. The harvested fruiting bodies in each bag were then counted and weighed. A total of
three flushes were recorded. In addition, mycelial growth rate, cropping days, yield,
and biological efficiency were recorded.

5. E = (wf /wd) × 100

6. Significant differences and mean values were analysed using one-way analysis of
variance (ANOVA)
 3
RESULTS
GROWTH RATE OF
OYSTER MUSHROOM
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YIELD
TOTAL YIELD
MUSHROOM LEAVES
BIOLOGICAL EFFICIENCY
GROWTH DURATION
 CONCLUSION
Tea waste of appropriate ratios (40–60%) could successfully be used as an oyster
mushroom cultivation substrate with high yield, biological efficiency and relatively
shorter cropping time. With these properties, as a waste with little utilisation value and
which even poses a threat to the environment, tea waste could be re-used efficiently and
economically as a new substrate in oyster mushroom cultivation which not only
converts remained energy into protein-rich food but also provides an environmental and
economic method of disposing of piles of tea waste from tea production plants and
industries.
THANK YOU