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Chromatography
Chromatography
Chromatography
Introduction of Chromatography
–The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure
holding another material called the stationary phase.
Principle of Chromatography
1 2 3 4 5
A horizontal line is drawn The paper is then placed When the solvent rises near the
near one end (about 1.5 into a sealed container end of the paper then the paper
cm from the bottom with a swallow layer of should be taken out from sealed
edge) of the paper suitable solvent. container and air dried. The paper
with separated bands of
components are then observed
under UV-light.
Uses of Chromatography