DETECTION OF MICROBIAL

CONTAMINATION
SARA ALI L1S17BSBT0031

MAHA FIDA L1S17BSBT0036

ASHBA HAIDER L1S17BSBT0049

 INTRODUCTION

• CONTAMINATION
• MICROBIAL CONTAMINATION
• SOURCE
• ORIGIN
 TYPES
• DIRECT CONTAMINATION
CONTAMINATION BY MICROBIAL COMPONENTS AND POORLY MAINTAINED HEATING,
VENTILATION AND AIR CONDITIONING HVAC SYSTEMS.

• INDIRECT CONTAMINATION
THIS IS HOW MICROBES SPREAD. SOME CELLS ARE CROSS CONTAMINATED BY CELLS OF OTHER
SPECIES.

PASSING OF MICROBES THROUGH IMPROPER AND UNSTERILIZED EQUIPMENT.

 IDENTIFICATION TECHNIQUES

• BIOLOG SYSTEM
• ANALYTICAL PROFILE INDEX (API)
• FATTY ACID ANALYSIS PROFILES (FAP)
• 16sRNA
 DETECTION PROCEDURES

• DETECTION BY BIOLIPID AND BIOCHEMICAL

METHODS
• DETECTION BY IMMUNOASSAYS
• DETECTION BY BIOSENSORS
 ANTIBIOTIC RESISTANCE

• ANTIBIOTICS MAYBE INACTIVATED BY ENVIRONMENTAL CONDITIONS LIKE

HEAT\LIGHT.
• ANTIBIOTIC SENSITIVITY CAN BE REDUCED IN PLANT TISSUE CULTURE MEDIA
DUE TO DIFFERENT FAVORABLE PH DEGREES.
• ANTIBIOTIC CONCENTRATION FOR A PARTICULAR BACTERIA SHOULD BE
DETERMINED.
• PHYTOTOXICITY VARIES GREATLY AMONG PLANT SPECIES AND EXPLANT TYPES
SO PRELIMINARY TESTING WITH PLANT CULTURES IS IMPORTANT
 REFRENCES
• HTTPS://
PDFS.SEMANTICSCHOLAR.ORG/8D94/DC356CA20C5E5521FBEBB252343D428032C0.P
DF
• HTTPS://SCIALERT.NET/FULLTEXT/?DOI=PPJ.2007.77.81
• HTTPS://WWW.NCBI.NLM.NIH.GOV/PMC/ARTICLES/PMC3371444/