RESEARCH PROPOSAL SEMINA

R
Presenter :
Amalia Nur Adiba ( D500164006)
Yuvita Mustika Fauzi ( D500164015)
THE EFFECT OF PARTICLE SIZE AND EXTRACTIONTEMPERATURE
ON THE CHLOROPHYLL CONCENTRATION IN THE PRODUCTION
OF NATURAL DYE FROM CASSAVA (Manihot utilisima) LEAVES

Amalia Nur Adiba ( D500164006)

 BACKGROUND

In the recent years, the use of natural dyes in textil

e industries is significantly growing.

Natural dye is an alternative subtitute for chemical dye, because

of the biodegradability, renewability, compatibility with the environ
ment and low allergic reaction.

The study aimed to determine the optimum chlorophyll concentration b

y variation of particle size and extraction temperature in the production
of natural dyes from cassava leaves. And to determine the absorbance
of
the dye solution before and after coloring.
Identify Problem
How to make cassava (Manihot utilisima) leaves as natural
1 dyes in textile using a simple aqueous extraction method?

How do the particle size and extraction temperature affect

2 the chlorophyll concentration in the production of natural
dyes from cassava (Manihot utilisima) leaves?

3 What are the absorbances of the dye solution before and after
the coloring?

4
Purpose of Research
To investigate the potential of cassava (Manihot utilisima)
1 leaves as textile natural dyes

To study the optimum chlorophyll concentration by the variation

2 of particle size and extraction temperature.

3 To know the absorbances of the dye solution before and after

the coloring

4
 Research Methodology
Independent Variable

Dependent Variable 01 Particle size

20, 40,and 60 mesh

The optimum chlorophyll concentration by the

variation of particle size and extraction temperature
in the production of natural dyes from cassava
Extraction temperature
(Manihot utilisima) leaves
02
30, 40, 50, 60, and 70 ℃
 Control Variable

01 Cassava leaves extract
Ratio of cassava leaves
02
and distilled water is 1 :4
Mordanting
40 gram of alum, meta-mordanting
process, immersion time is 15
minutes

Type of fabric Dyeing

03 cotton fabric with a size of
12 cm x12 cm
04 Dyeing time is 20 minutes
repeat 3 times
 Research Design
The research design used is 2 factors completely randomized design.
Table 1. Research Design
 
Particle Size (mesh)
Extraction Temperature (0C)

60 70
30 40 50

20 y11 y12 y13 y14 y15

40 y21 y22 y23 y24 y25
60 y31 y32 y33 y34 y35
 Data Analysis

The effect of particle size and temperature extraction to the

chlorophyll concentration in the production of natural dyes
from cassava (Manihot utilisima) leaves is determined using
two ways analysis of variance
 Procedure
a. Preparation of cassava leaves solution

10 gram of (20, 40, 60 mesh)

cassava leaves that Scale blender mesh
has been washed
Dissolved with 40 ml
of distilled water
Electrical Beaker
stove glass

Temperature variation : Soak for 12 hour

30, 40, 50, 60, and 70 C
For 15 minutes
 Procedure
b. Making chlorophyll extract

1.5 ml cassava
Mixture of cassava Filter leaves solution
Beaker
leaves solution paper glass
8.5 ml of acetone

UV-Vis (Left for 12

Refrigerator
spectrophotometer hour )
 Procedure
c. Process of mordanting and dyeing

2 ml of liquid soap in
Cotton fabric 100 ml distilled water
12x12 cm, 3 pieces
Waterbath

40 gram of alum
Beaker
1000 ml of glass
distilled water

Beaker UV-Vis
100 ml of glass
dye solution
spectrophotometer
Put the cotton fabric
into mixture solution
for 20 minute,the dry it
THE EFFECT OF MORDANT CONCENTRATION
AND MORDANTING PROCESS ON CASSAVA
(Manihot utilisima) LEAVES AS TEXTILE
NATURAL DYE

Yuvita Mustika Fauzi ( D500164015)

Identify Problem
How to make cassava (Manihot utilisima) leaves as natural
1 dyes in textile using a simple aqueous extraction method?

How the effect of mordant concentration and process of mordanting

2 affect the strength and color effectiveness in the coloring on cotton
fabric using cassava (Manihot utilisima) leaves as natural dyes?

4
Purpose of Research
To obtain the potential of cassava (Manihot utilisima) leaves as
1 textile natural dye

To obtain the color strength and effectiveness of mordant

2 concentration on cotton textile using Cassava (Manihot utilisma)
leaves as natural dye

4
 Research Methodology
Independent Variable

Dependent Variable 01 Concentration of mordant

2%, 3%,and 4% in 1000 ml
To get the fabric with the best result of the
strength and color effectiveness from testing
the absorbance value of the dye after dyeing

Mordanting
02 • Pre-mordanting
• meta-mordanting
• Post-mordanting
 Research Design
The research design used is 2 factors completely randomized design.
Table 1. Research Design
 
Mordanting Process Type of Mordant (Y)
(X)

Y1 Y2 Y3

Pre-mordanting (X1) X1Y1 X1Y2 X1Y3

Meta-mordanting (X2) X2Y1 X2Y2 X3Y3

Post-mordanting (X3) X3Y1 X3Y2 X3Y3

 Data Analysis

The absorbance determination is determined by measuring

the natural dye solution. Effect mordant concentration and
Mordanting process and their interaction with absorbance
numbers are determined by ANOVA two-way.
 Procedure
a. Preparation of cassava leaves solution

250 gram of
cassava leaves that Scale blender mesh
has been washed

Dissolved with 1000 ml

of distilled water

Electrical Beaker
stove glass
Temperature variation : optimum Soak for 12 hour
For 15 minutes
 Procedure
b. Preparation of mordant solution

Beaker
20, 30, 40 gram of Allum Scale Dissolved with 1000 ml glass
of distilled water

Electrical
stove
Temperature : 50 ℃
 Procedure
c. Process of pre-mordanting

Soak into mordant solution

Cotton fabric for 15 minutes Beaker Wash using
12x12 cm, 3 pieces distilled water
glass

UV-Vis Beaker Soak into natural dye

solution for 20 minutes
spectrophotometer glass

Dry cotton
 Procedure
d. Process of meta-mordanting

Mordant 100 ml Natural dye 100 ml

Soak into natural dye

solution for 20 minutes

Water bath

Dry cotton

Uv-vis spectrophotometry
 Procedure
e. Process of post-mordanting
Soak into natural dye solution
Cotton fabric for 15 minutes Beaker
12x12 cm, 3 pieces
glass
Dry cotton

UV-Vis Beaker Soak into mordant

solution for 20 minutes
spectrophotometer glass

Dry cotton
Picture of UV-Vis Spectrophotometer
Thank you