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Physiology of Retina: Dr. Preeti Agarwal (1 Year Resident)
Physiology of Retina: Dr. Preeti Agarwal (1 Year Resident)
Physiology Of Retina
Presentation layout
• Introduction
• Physiology of the RPE/ Neural Retina
• Visual Cycle
• Phototransduction
• Information processing within the retina
• Light /Dark adaptation
3
RETINA
Absorption of light
• As a pigment layer of cells , it absorbs scattered
light to improve the optical quality.
• Light is absorbed by the melanin granule of
RPE leading to increase in temperature of the
RPE choroid complex.
• The heat is transported away by the bloodstream
in the choriocapillaris.
8
Retinal Pigments
• Melanin
• Contain within the cytoplasmic granules of
melanosomes
• In old age, these pigments fuse with lysosome and
break down
• Absorb stray lights and minimize scatter within the eye
• Serves as a free radical stabilizer, bind toxins and
retinotoxic drugs
Tesselated fundus
10
Retinal Pigments
• Lipofuscin
• Accumulates gradually with age
• Derived from
- the outer segment lipids that have
been ingested and then digested by
the RPE
- membrane fragments that have
been damaged by the light or
oxidation
• Clinically seen as Drusens
11
Transepithelial transport
RPE constitute a monolayer of cells; cuboidal in cross
section and hexagonal when looked from above
Photoreceptors Free
Radicals Damage Membranes
Production Over time
16
Secretion
• The RPE is capable of secreting a large variety of growth factors,
cytokines or immune modulators.
• Functions:
vascular supply
permeability
growth
repair
other processes vital to retinal function
20
Growth Factors
Platelet Derived Growth Factor Modulates cell growth and healing
(PDGF)
AMD
Atrophic Form Neovascular Type
• Characterised by • RPE and possibly
Insufficient production of photoreceptors produce
survival factors by damaged excess VEGF
RPE • Stimulates outgrowth of new
• Leading to apoptosis of capillaries , CNV
functional complexes formed • Clinically antagonists of VEGF
by choriocapillaries, RPE and are injected in exudative AMD,
often stabiling or even
photoreceptors
improving vision
23
Retinal Adhesion
• Interphotoreceptor matrix (IPM) contain glycosaminoglycans
(GAGs) which surround rod and cones
• IPM functions:
• - physical support to the photoreceptors
• - transfer of nutrients and visual pigments
• - formation of an adhesive bond between retina and RPE
• The IPM function is largely controlled by RPE through
synthesis of matrix materials and transport proteins and also
through transport of ions and water.
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OTHER MECHANISM
• Vitreous gel
• Intraocular fluid pressure
• RPE water transport
• Mechanical interdigitation
Schaffer’s sign
26
Valuable in?
Dependent upon
• The ability of RPE cells to seal laser scars, re-establish
a degree of normal transport and avoid unnecessary
leakage of proteins into the subretinal space.
29
30
4 to 6 million cones
75,000 rods
Pathology
• Retinitis Pigmentosa
• Progressive Cone Dystrophy
36
RHODOPSIN
• Present in outer segment of Rods
• OPSIN + RETINAL= RHODOPSIN
• It is insoluble in water
• Sensitive to acid and alkalis
• Absorbs yellow wavelength of light, transmits
violet to red colour, hence appears visual purple.
37
Cone pigments
• 3 kinds:
• CYANOLABE : blue sensitive 435nm
• CHLOROLABE : green sensitive 535 nm
• ERYTHROLABE : red sensitive 580 nm
• Responsible for colour vision
• Cone pigments are different from Rhodopsin in
opsin portion , 11-cis retinal is same as
Rhodopsin
Colour Blindness
Normal colour vision uses all three types of light
cones correctly known as Trichromacy
People with “faulty” trichromatic vision will be
colour blind
The different anomalous conditions are :
• PROTANOMALY RED-GREEN COLOUR BLIND
• DEUTERANOMALY
• TRITANOMALY
NORMAL
RED- GREEN
BLUE DEFECT
DEFECT
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Opsin
Long helix
348 amino acids
• Loop seven times
• Determines the
wavelength absorbed by a
photoreceptor
• 11-cis-retinal, derivative of
vitamin A
41
Phototransduction
• It is Series of biochemical events :
Release of
Photons captured Hyperpolarization
Neurotransmitter
VISUAL CYCLE
&
PHOTOTRANSDUCTION
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BLEACHING
REGENERATION
44
Change in Opsin
Configuration
Transducin (Gα)
is activated
α-Subunit separates
46
Subunit activates
cGMP PDE
Converts cGMP to
5’-GMP
Reduced cytoplasmic
cGMP
Closure of leaky
Na+ Channels
Decrease intracellular
Ca2+
Decrease Glutamate
release
Depolarization Hyperpolarization
(On Center bipolar (Off center bipolar
cells) cells)
49
Bipolar Cells
• 1st order neuron in visual pathway
• Once a threshold is reached, the ganglion cell
responds and a signal is sent to higher CNS
locations
• Rod bipolars do not synapse with ganglion cells
directly but with amacrine cells
• TYPES
1. On Bipolar cells
2. Off Bipolar cells
50
Amacrine Cells
• Receive information at
the synapse of the bipolar
cell axons with the
ganglion cell dendrites
• Bipolar cells project onto
both ganglion and
amacrine cells
• Negative feedback
• Reciprocal inhibition
• Works laterally
55
Ganglion Cell
• Electrical response of bipolar cells after modification by
the amacrine cells
• Transmit the information by means of action potential
Receptive Fields
Arranged in a center-surround pattern
The center-surround
response occurs in part due
to
• Lateral inhibition by
horizontal cells
VISUAL ADAPTATION
Very quick
• 1 minute : 10 x sensitivite
Mechanism
• Visual pigment mechanism
• Other mechanisms
• - Change in pupil size
• - Neural adaptation
Feedback inhibition
Oguchi’s disease:
• prolonged rod dark adaptation
• Despite normal rhodopsin
regeneration
• Demonstrate neural mechanism of
dark adaptation
67
References
Adler’s Physiology Of The Eye – 11th Edition
Anatomy And Physiology Of Eye - 2nd Edition- A. K
Khurana
Yanoff & Duker Ophthalmology - 4h Edition
Clinical Ophthalmology - 8th Edition- J. Kanski
AAO Retina and Vitreous - Section 12
AAO Fundamentals and Principles of Ophthalmology -
Section 2
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