IBR-UrBioTect™

-
Natural protection
from Urban Pollution

Urba Bi Protec
n o t
 URBAN POLLUTION
Contamination of both indoor and outdoor environments

AIR POLLUTION RADIATION POLLUTION

 Industry‐ power plants  Cell Phones
 Vehicles (fuel exhaust)  Cell Phones towers
 Cigarettes smoke  TV’s
 Fires  Computers
 Heating with wood, gas...  Microwaves
 Paintings (VOC’s)  Ovens
 Agriculture (pesticides)  Wireless internet
 Air Conditioning & Dust
AIR POLLUTION IS A REAL GLOBAL THREAT
• 95% of the world's population is in contact with polluted, unsafe, dangerous air particles:
PM2.5, PM10 (fine particles) , PAHs (polycyclic aromatic hydrocarbons), (VOCs) volatile organic
compounds, nitrogen oxides, cigarette smoke and heavy metals

• 91% of the world’s population lives in places where air quality exceeds WHO guideline limits
• 99% of the urban population in China, India and Pakistan are regularly exposed to PM2.5
concentrations higher than those recommended

Everyone suffers from air pollution!

BLUE LIGHT RADIATION IS A RISING THREAT
• Blue light = High Energy Visible (HEV) light,
is the 390‐500nm wavelength range of the
electromagnetic spectrum

• People are exposed to Blue light both

outdoors and indoors

• Emitted from the sun, but through the

digital devices such as cell phones,
computers, tablets and flat‐screen TVs

• Blue light penetrates deeper into the

dermis compared with UVA and UVB

• Blue light damaging effects are cumulative

over the course of a lifetime.
 POLLUTION IS A GLOBAL CONCERN… AFFECTING THE SKIN
 People are recognizing the dangers of air pollution and are looking for ways to protect themselves and
their skin

 People are also concerned about the impact of Blue light pollution and are looking for ways to protect
their skin from Blue light damage

 Better consumer awareness of the impact of external pollution on the skin!

68-88% 20% 55%

Of Chinese consumers
of women in France, would like to learn more
of Brazilians show
Germany, Italy and Spain about how to protect
interest in using anti-
agree that it is necessary themselves
pollution facial skincare
to protect the skin from
pollution
products
61%
concerned about PM2.5
Mintel:
Base:female internet users aged 16+ (France: 1,034; Germany: 1,009; Italy: 1,017; Spain: 1,008); 2018; * GNPD keyword search for "pollution" in the description field with word variants
POLLUTION HOW IT AFFECTS THE SKIN
SUNLIGHT
POLLUTION BLUE LIGHT

Weaker Skin
Barrier

Signs Loss of
of Radiance
Agin &
g Dullness
(wrinkles &
loss of
firnmess) Carbonylate
d Proteins

Inflammator
y Cascade

ECM
Degradation

HEALTHY SKIN SKIN SUBMITTED TO POLLUTION

NATURE HOLDS THE SOLUTION!
IBR‐URBIOTECT™
Inula Helenium Extract

The name Traditional medicine usage

the plant grew where  Strengthen & support respiratory system
Helen of Troy's tears  Anti oxidative and anti inflammatory power
fell when she was  Fresh leaves are used for wound healing
abducted by
 Leaves infusion is used for dry skin
Paris

• Native to Europe & Asia

• Flowers bloom from Jul through Feb (in Israel)

• Hand harvested annually during Q3

IBR‐URBIOTECT™‐ UNIQUE TRENDY SOLUTION FOR THE MODERN LIFE STYLE
Inula Helenium extract able to protect the skin from urban pollution & blue light damage
for healthy, radiant, hydrated and smooth skin
 IN VITRO & EX VIVO STUDIES

1 MODULATION OF GENE EXPRESSION

PROTECTION FROM BLUE LIGHT DAMAGE

PROTECTION FROM DIESEL FUMES DAMAGE
2
PROTECTION FROM CIGARETTE SMOKE
PROTECTION FROM HEAVY METALS
GENE EXPRESSION ON SKIN BARRIER GENES 1
In vitro protocol
• Normal Human Epidermal Keratinocytes (NHEK) were incubated for 24 hours with IBR‐UrBioTect™ 0.33%/untreated
• Extraction of RNA followed by DNA ARRAY analysis (U219 array ‐ containing 30,000 transcripts)
• Significance set at 2 fold change

IBR‐UrBioTect™ modulates genes involved in optimal skin barrier function

 GENE EXPRESSION ON ECM PROTEINS 1

IBR‐UrBioTect™ modulates genes involved with ECM integrity leading to reduction in the
appearance of premature aging signs
GENE EXPRESSION ON INFLAMMATION MARKERS 1

IBR‐UrBioTect™ modulates gens involved in the inflammatory response

 PROTECTION FROM BLUE LIGHT INDUCED ROS 2
In vitro protocol
• Human keratinocytes (HaCaT) were incubated with IBR‐UrBioTect™ at 0.125%, 0.25% and 0.5% for 24hrs
• Cells were exposed to Blue light (HEV) for 60 minutes (2 J/cm2) (free radicals generation)
• The intracellular ROS were measured by fluoromertic product that its amounts are proportional to the amount of ROS present. Fluorescence
quantification was measured at λex=490/ λ em=525.

Blue light‐induced oxidative stress in live skin

Author links open overlay
panelYuyaNakashimaShigeoOhtaAlexander M.Wolf
Free Radical Biology and Medicine

IBR‐UrBioTect™ reduces Blue light induced ROS, thus helps protect from
blue light damage & digital pollution
 OXIDATIVE STRESS DUE TO BLUE LIGHT TRIGGERS A PROCESS 2
KNOWN AS PROTEIN CARBONYLATION

Vemula et al., 2015

• Carbonylation refers to reactive carbonyl groups present in biomolecules due to oxidative reactions induced by reactive
oxygen species.
• Protein carbonylation, one of the most harmful irreversible protein modifications, is considered as a major hallmark of
cellular oxidative stress.
• Carbonylated proteins in the stratum corneum compromise skin barrier and impact skin function including water content
and transepidermal water loss (TEWL).
 PROTECTION FROM PROTEIN CARBONYLATION 2
Ex vivo protocol
• Human skin explants from a 22‐year‐old Caucasian woman (phototype II).
• Chronic exposure to Blue light (HEV) for 3 hours (63,7 J/cm²) delivered by the Solarbox® ‐ daily for 10 days.
• 1% IBR‐UrBioTect containing gel applied to the skin explant daily 15 minutes prior to blue light irradiation.
• Oxidative tissue damage was measured @ D10 by analyzing protein carbonylation.
Chronic exposure to Blue light
Untreated /No Blue light Untreated 1% IBR‐UrBioTect

D10 EpiD

IBR‐UrBioTect™ induces a clear decrease in protein carbonylation induced by chronic

exposure to Blue light (HEV)
PROTECTION FROM DIESEL GAS INDUCED INFLAMMATION 2
In vitro protocol
• Human normal keratinocytes were pre‐incubated for 24 hours in the absence (control) or in the presence of increasing concentrations (3) of
IBR‐UrBioTect™ : 0.03%, 0.1%, 0.3%.
• Cells were incubated for additional 24h with the presence of culture medium “polluted” with diesel gas exhaust .
• IL‐8 content in the culture medium was quantified by using a specific ELISA assay.

IBR‐UrBioTect™ reduces Diesel gas induced inflammatory response,

thus helps protect from air pollution
PROTECTION FROM CIGARETTE SMOKE INDUCED INFLAMMATION 2
Ex vivo protocol
• Human skin explants from a 59‐year‐old Caucasian man treated daily with 1% IBR‐UrBioTect for 4 days.
• Exposure to cigarette smoke (2 cigarettes) for 2 hours in PolluBox system at day 5.
• Quantification of IL1a was measured in culture medium at Day 6.

IBR‐UrBioTect™ reduces IL1‐ induced inflammatory response,

thus helps protect from air pollution
 PROTECTION FROM CIGARETTE SMOKE INDUCED BARRIER DISRUPTION 2
Ex vivo protocol
• Human skin explants from a 59‐year‐old Caucasian man treated daily with 1% IBR‐UrBioTect for 4 days.
• Exposure to cigarette smoke (2 cigarettes) for 2 hours in PolluBox system at day 5.
• Immunostaining of Caspase 14 at Day 6.

W/O cigarette smoke W cigarette smoke

Untreated Untreated 1% IBR‐UrBioTect

Caspase 14

EpiD

IBR‐UrBioTect™ increases Caspase 14 expression,

thus protects from skin barrier disruption in polluted environment
PROTECTION FROM HEAVY METALS 2
In tubo protocol
• Ferrous iron‐Ferrozine complex is measured by spectrophotometry (absorbance at 562 nm) (Rohman et al. (2010)
• Chelating power is expressed as an IC50 value (mg/ml) = concentration of tested product at which 50% of available iron was chelated.

IC50
Sample (mg/ml Dry Weight)

Inula Helenium 0.13

Inula Garveolens 0.67
Inula Viscosa 1.25
Asteriscus Graveolens 1.7

IBR‐UrBioTect™ neutralizes heavy metals, thus reducing their toxicity

 CLINICAL STUDY

In vivo protocol
•26 women (age 46‐60) with heavy smoking habits (>10 cigarettes / day) and skin INCI/chemical name % in formula Placebo
aging signs & skin type & phototype II to III Water 84.35 84.85
•Application 2X/D for 4 weeks of gel‐cream containing 1% of IBR‐UrBioTect™ or Butylene glycol 4.00 4.00
placebo (split face) Dipropylene glycol 1.00 1.00
• Evaluation of skin barrier‐ TEWL (malar area) Hexylene glycol 1.00 1.00
•Evaluation of wrinkles using a Visia (periorbital area) & evaluation of skin Polysorbate 20 1.00 1.00
elasticity with Cutometer (corner of the mouth) Hydrogenated polydecene 1.50 1.50
• Evaluation of skin lightening using a Chromameter (periorbital area) Cyclomethicone 4.00 4.00
IBR‐UrBioTect™ 1.00 0.00
Glycerol 0.00 0.50
Carbomer 0.80 0.80
Triethanolamine 0.70 0.70
Phenoxyethanol 0.40 0.40
Methyl paraben 0.15 0.15
EDTA 0.10 0.10
TOTAL 100.00 100.00
EVALUATION OF SKIN BARRIER INTEGRITY SUNLIGHT POLLUTION BLUE LIGHT

IBR‐UrBioTect™ increases the skin barrier function to seal the skin from pollutants
and improve hydration
 EVALUATION OF SIGNS OF PREMATURE AGING

IBR‐UrBioTect™ helps to reduce the appearance of wrinkles and improves elasticity, major
signs of pre‐mature aging, resulting from exposure to pollution
EVALUATION OF SKIN DULLNESS

100% positive response!

maximal improvement
Up to 107%

IBR‐UrBioTect™ increases skin radiance and lightening, indicators of healthy

and younger looking skin
VISIBLE IMPROVEMENT‐ LIGHTER AND BRIGHTER SKIN TONE
WITH SMOOTHER & HEALTHIER APPEARANCE

D0 D28 D0 D28

IBR‐UrBioTect™ a multifunctional ingredient shielding pollution away

for a healthy beautiful look
IBR‐URBIOTECT®‐ MODE OF ACTION
Moisturized, Radiant,
Healthy & young looking skin
+ IBR‐UrBioTect®
Modulates genes
involved in optimal skin
barrier function,
inflammation & ECM
integrity

Protection from protein

carbonylation induced by
chronic exposure to Blue
light

Protection from Blue

light induced ROS

Inhibition of diesel gas

induced IL‐8 production

Inhibition of cigarette
SKIN SUBMITTED TO POLLUTION smoke induced IL1‐ α
production
 IBR‐URBIOTECT™‐ UNIQUE TRENDY SOLUTION FOR THE MODERN LIFE STYLE
Inula Helenium extract is able to protect the skin from urban pollution & blue light damage for healthy, radiant, hydrated
and smooth skin
MULTIPLE PROTECTION STRATEGIES
• Promotes strengthening & integrity of skin barrier
• Helps decreasing the inflammation response
CLINICALLY PROVEN
• Improves hydration, skin radiance & elasticity
• Reduces diesel fumes / cigarette smoke induced
• Helps to Reduce appearance of wrinkles
inflammation response
• Reduces blue‐light (HEV) induced ROS/oxidative stress
damage (protein carbonylation) CONSUMER BENEFITS
• Protects from heavy metals • Helps protecting from multiple urban
pollutants and blue light damage
• Delays premature aging signs in urbanized
environment
ORIGIN
MANUFACTURER BENEFITS
• Inula Helenium extract
• Natural active ingredient
• Fully traceable • 1 ingredient= multiple applications
• Neutralize pollutants: perfect for cleansing &
detox claims
• Suitable for men & women of all ages
PRODUCT INFORMATION
INCI NAME Glycerin (and) Water (and) Inula Helenium Extract
ADDITIVE Benzyl Alcohol & Benzoic Acid
APPEARANCE Aqueous Light brown to brown solution
FORMULATIO Should be incorporated at the end of the
formulation at a temperature below 40oC
N
DOSAGE Preventive action: <0.5
Intensive care: 0.5‐1 %
OPTIMUM PH 5.0‐9.0
APPLICATION • Protective care • Day care
• Detoxifying care • Moisturizing care
S
• Anti‐pollution • Anti‐aging care
• Urban/City defense skin care • Men care
• Cleansing care • Makeup
• Mask

COSMOS CERTIFIED CHINA COMPLIANT ECO‐FRIENDLY

APPLICATIONS – CLAIMS

To feel unstoppable

To feel beautiful everyday

To feel protected

Dosage: Applications:
• Cleansing care & Mask
• Intensive treatment: 1% • Skin care
• Day care
• Preventive action: 0.5% • Anti‐pollution & Protective care
• Moisturizing care
• Detoxifying care
• Anti‐aging care
• Urban/City defense skin care
• Men care
• Makeup

29
TOXICOLOGICAL STUDIES
 Eye Irritation (Neutral Red Release) – IBR‐UrBioTect® tested at 30%
 Skin tolerance (48‐h patch test) ‐ IBR‐UrBioTect® tested at 100%
 Skin sensitization (HRIPT) ‐ IBR‐UrBioTect® tested at 100%
 Mutagenicity (Ames, OECD 471) ‐ IBR‐UrBioTect® tested at 100%
 Phototoxicity (OECD 432) – IBR‐UrBioTect® tested at 30%

ECOTOXICITY
 On going

Excellent safety profile

THANK YOU!