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    PMI Project

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    Subhan Ali
    1. 500g of baker's yeast was suspended in 2L of deionized water to prepare an aqueous yeast cell suspension. 2. The suspension was autoclaved, centrifuged, and autoclaved again to remove insoluble cell contents and produce a supernatant containing soluble components. 3. The supernatant was spray dried to obtain a powdered yeast extract, which was checked for quality by growing bacteria in media containing the extract.

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    PMI Project

    Uploaded by

    Subhan Ali
    21 views2 pages
    1. 500g of baker's yeast was suspended in 2L of deionized water to prepare an aqueous yeast cell suspension. 2. The suspension was autoclaved, centrifuged, and autoclaved again to remove insoluble cell contents and produce a supernatant containing soluble components. 3. The supernatant was spray dried to obtain a powdered yeast extract, which was checked for quality by growing bacteria in media containing the extract.

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    1. 500g of baker's yeast was suspended in 2L of deionized water to prepare an aqueous yeast cell suspension. 2. The suspension was autoclaved, centrifuged, and autoclaved again to remove insoluble cell contents and produce a supernatant containing soluble components. 3. The supernatant was spray dried to obtain a powdered yeast extract, which was checked for quality by growing bacteria in media containing the extract.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pptx, pdf, or txt
    21 views2 pages

    PMI Project

    Uploaded by

    Subhan Ali
    1. 500g of baker's yeast was suspended in 2L of deionized water to prepare an aqueous yeast cell suspension. 2. The suspension was autoclaved, centrifuged, and autoclaved again to remove insoluble cell contents and produce a supernatant containing soluble components. 3. The supernatant was spray dried to obtain a powdered yeast extract, which was checked for quality by growing bacteria in media containing the extract.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
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    Download as pptx, pdf, or txt
    of 2

    Preparation of yeast extract from

    Saccharomyces cerevisiae for bacterial


    culture media
    Maryam iqbal
    Roll. No 57
    Department: Botany
    Plant microbe Interaction
    Aqueous
    1 suspension of 2
    yeast cell
    prepared

    Prepared
    500g baker’s yeast (Saccharomyces cerevisiae) suspension
    + 2L deionized water
    Supernatant re-
    3 autoclaved at
    5 centrifuged again 4
    115°C for 10
    at same Cell debris mintues followed
    conditions to make separation in by colling on ice
    sure of removing centrifuge
    insoluble cell machine at
    content 400gat, 4°C for 10
    minutes

    Supernatant
    Produced water
    containing
    soluble extract 8
    soluble Soluble fraction
    stored at – 20°C Powdered yeast extract
    Components subjected to mini spray
    untill preparation ready for bacterial culture
    re-autoclaved dryer (B-290) for
    of yeast extract in
    and cooled 6 powder form 7 obtaining powder form
    media. Quality of this
    extract checked by growing
    again of yeast extract bacteria in this.

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