MLAB 1415: Hematology

Micros 60
 Micros 60
• 3-part differential
• Sample size= 10µL
• Environment
– Indoor only
– Level work station
– Avoid direct exposure to sunlight
– Proper ventilation
– 60-90oF
• Linearity (AMR)
– WBC (103/mm3): 0-100
– RBC (106/mm3): 0-8.0
– Hgb (g/dL): 2.0-27
– Hct (%): 1.8-82.3
– Plt (103/mm3): 25-4990
 Parameters
Parameters Measurements/Calculations
• WBC • WBC/RBC/PLT
• RBC
• Hgb – Impedance change
• Hct • HGB
• MCV
• MCH – Spectrophotometry
• MCHC • LYM%/MON%/GRA%
• RDW
• Platelet – Impedance change
• MPV
• Lym #
• Lym%  Calculations
• Mono # • MCV,
• Mono%
• Gra#
MCH,MCHC,RDW,MPV,LYM#,
• Gra% MON#, GRA#
 Stats

Principle of operation: The counting principle used for the red blood cells, white blood
cells and platelets is based on the variations in impedance generated by the passage
of cells through a calibrated aperture.
Number of samples which can be analyzed per hour: 60
Number of tests performed on this analyzer: 1
Quality control: Three levels run each day of patient testing, or following instrument
repair, reagent lot change, or when indicated.
Calibration: Shift in control values, replacement of instrument parts, reagent number
change, or when indicated
Preventative maintenance: Daily: start up and shut down, general cleaning
Acceptable samples: whole blood from an EDTA tube ran within 4 hours of collection
Limitations: Lipemia, cold agglutinins, high WBC’s
 Equipement
• 8 Main parts
– Electrical supply
– Electronic board
– Dilution pneumatics
– Control panel with
keyboard and LCD screen
– Reagent compartment
– Printer
– Smart card reader
– Bar code reader (optional)
 Reagents
– Diluent
• ABX minidil: sodium hydroxide/azide
• Determines and differenciates blood cells
– Cleaner
• ABX miniclean: enzymatic detergent
• Cleans system
– LYSE: potassium cyanide
• ABX Alphalyse: Lyses RBCs, releasing hemoglobin and
WBCs. WBCs are counted by impedance.
• Waste
 Distribution curves
• WBC
• RBC
• Plt
 Daily Start Up
1. Turn on instrument, once on, the start-up will begin automatically
1. Instrument is performing a cleaning and rinsing cycle:
detergent in the chamber is rinsed with diluent
2. Cycle lasts about 130 seconds
2. At cycle completion, background values will be displayed. These
values must be less than the below:
1. WBC < 0.3 x 103/mm3
2. RBC < 0.02 x 106/mm3
3. Hgb < 0.0 g/dL
4. Plt < 10x 103/ mm3
5. If the values are not within this range, another cycle will begin.
If still not in range, the Micros will do another start-up. After
3 failed cycles, it will give a “start-up failed” message.
Troubleshooting will now start.
 Daily start-up con’t
3. Run a primer sample
4. Perform QC and evaluate
5. Complete maintenance sheet
6. If all QC in, can continue with patient
samples.
 Running a Patient
1. Press the ID button on
the key pad
2. Manually enter the ID.
Use the up and down
arrows to enter
alphabetical
characters. Please
Enter after each entry.
 Shutdown
1. Hit the Stand by key,
wait 1 minute
2. Switch off instrument
and printer.