UNIT 2 MICROBIAL CONTROL

IN VITRO CONTROL OF
MICROBIAL GROWTH
ontrol them before they control us.
Lesson 1 – In Vitro Control of Microbial Growth

•List several factors that affect the

growth of microorganisms.
•Describe how to encourage in
vitro growth of microorganisms.
•Describe how to inhibit in vitro
growth of microorganisms.
FACTORS THAT AFFECT MICROBIAL GROWTH

•availability of •osmotic
nutrients pressure
•moisture •barometric
•temperature pressure
•pH •composition of
the atmosphere
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Microbes and Temperature
•Thermophiles
prefer living in hot temperature
•Mesophiles
prefer moderate temperatures
•Psychrophiles
prefer cold temperatures
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Microbes and Temperature
•Psychrotrophs
prefer living in refrigerator
temp. of 4°C
•Psychroduric organisms
prefer warmer temperatures but
can endure cold temperatures and
can be preserve in frozenMICROBIAL
state CONTROL
Microbes and Other Factors
•Acidophile
prefer acidic environment
•Alkaliphile
prefer alkaline
environment
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Microbes and Other Factors
•Halophile
live in salty environment
•Barophile
live in environments with
high barometric pressure
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 Microbes are cultured in
laboratories and industries.

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Culture Media
a solid, liquid or
semi-solid
designed to
support the
growth of
microorganisms
or cells; also
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Types of Culture Media
•Enriched Media
contains rich amount of nutrients
•Selective Media
contains inhibitors that discourage
growth of certain organisms
•Differential Media
permits differentiation of mircobes
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MICROBIAL CONTROL
Population Growth

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INHIBITION OF
MICROBIAL
•Sterilization
GROWTH
complete destruction of all
microbial life
•Disinfection
destruction of pathogens, e.g.
pasteurization and antiseptic
techniques MICROBIAL CONTROL
Physical Methods to Inhibit Microbial
Growth

Moist
Dry Heat
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Physical Methods to Inhibit Microbial
Growth

Radiati
Desiccati onMICROBIAL CONTROL
Physical Methods to Inhibit Microbial
Growth

Ultrasoni Filtrati
c waves on
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Chemical Disinfection
The use of chemical agents to
inhibit the growth of pathogens,
either temporarily or permanently.

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Lyophilization
also called as freeze-drying is a
method used in preserving
microbes for future use

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