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Eglamour Cell Disruption
Eglamour Cell Disruption
Eglamour Cell Disruption
DISRUPTION
Oleh :
Yustina (NPM 1706123141)
Fitria Afriani (NPM 1706122901)
Mata Kuliah Bio Separasi
Outline
2
Introduction
3
Introduction (cont’d)
Cell disruption is an essential part
of biotechnology and the
downstream processes related to
the manufacturing of biological
products
4
Introduction (cont’d)
So, now let’s see the structure of a cell
5
Plant Cell vs Animal Cell
So, now let’s see the structure of a cell
6
Introduction (cont’d)
So, now let’s see the structure of a cell
7
Method of Microbial Disruption
9
Ideal Technology Characterization (cont’d)
10
11
Methods of Microbial Disruption
Non Mechanical
Non Mechanical
Physical Methods
Thermolysis, Decompression,
Chemical Methods
Solvent, Detergents
Osmotic Shock
Cell
Disruption 12
Product of interest : Intracellular or Extracellular ?
13
1. Mechanical Methods
14
Disadvantages Cell Disruption Methods
15
a. High Pressure Homogenization
16
cont’d....
Advantages :
• Customizable forces
to optimize results
• Hot and Cold
homogenization option
• Smaller particle sizes
than other equipment
• Aplikasi pada industri
susu, pembuatan nano
emulsi, dll
17
a. High Pressure Homogenization (cont’d)
18
a. High Pressure
19
b. French Press
20
cont’d...
21
c. Ultra sound/sonication
22
Let’s start with the first set of slides
23
d. Bead Mills
a. High Pressure
25
26
Thermolysis
Thermolysis has shown potential in becoming more
common in large scale production.
Periplasmic proteins in G(-) bacteria are released
when the cells are heated up to 50ºC.
Cytoplasmic proteins can be released from E.coli
within 10min at 90 ºC.
Improved protein release has been obtained after
short high temperature shocks, than when at longer
temperature exposures at lower values.
The results are highly unreliable, as the protein
solubility changes with temperature fluctuations.
Decompression
Exposure of cells to either high or low salt concentration causes cell disruption
Title???
30
Title???
31
a. High Pressure
32
Detergents
Anionic :
sodium dodecyl
sulfate (SDS)
Non-ionic :
triton X100
Cationic : ethyl
trimethyl
ammonium
bromide
Enzymes degrade the cell wall components which will lead to release of
intracellular compounds.
Application
Cont’d.... Combined Enzymatic and Mechanical Cell Disruption and
Lipid Extraction of Green Alga Neochloris oleoabundans
Cont’d....
Cont’d....
Enzymatic lysis
of microbial cells
conclusion
Cell disruption is an essential part of biotechnology and the
downstream processes.
Metode cell disruption terdiri dari mechanical methods meliputi high
pressure homogenization, french press, sonication, bead mill, grinding,
non mechanical physical methods meliputi thermolysis,
decompression, osmotic shock, non mechanical chemical methods
meliputi solvents and detergents, non mechanical biological methods
meliputi enzymes.
Application of cell disruption is ...... Excretion of .....