Glycerol Production By Microbial

Fermentation

By - Ajay Kumar
MSc. Biotech II Sem.
 Key points

Introduction

Functions of Glycerol In cell Membrane

Glycerol Production by S. Cerevisiae

Uses Of Glycerol

Genetic Improvements of glycerol
Synthesis

Conclusion and Future Prospects.
 Introduction about Glycerol

Colorless , Odorless , viscous liquid

Sweet test and non-toxic

Have Antimicrobial and antiviral
properties

Formula C3H8O3

Molar mass- 92.09382g/mol

Boiling point- 290 degree Celsius

Ph 7

Viscosity – 1.4 pa
 Function of Glycerol In Cell Membrane

1. Osmoregulation -
Glycerol plays an essential role as a compatible solute
during osmoregulation in yeasts . In response to decreased extracellular
water activity, S. cerevisiae greatly increases its rate of glycerol formation .
2. Redox balancing -
Glycerol is produced by S. cerevisiae during
fermentation of glucose to ethanol in order to maintain the redox balance.
 Production By S. Cerevisiae
Glycerol is the main compatible solute in yeast
saccharomyces cerevisiae, When faced with osmotic stress,
for example during fermentation, yeast cells produce and
accumulate glycerol in order to prevent dehydration by
balancing the intracellular osmolarity with that of
environment.
 Use of Glycerol

Cosmetics and medicinal preparation

Production of printers roller, Ink in
rubber stamp

Keeping tabacco moist and leather
soft

Food products , sweetener

Skin hair soap products ,
mouthwashes & toothpaste

Manufacture of explosives

Antifreeze
 Genetic Improvements
Mutagenesis, strain breeding or
overexpression of the genes associated
with glycerol formation has been
attempted in efforts to improve
glycerol synthesis by microorganisms.
Almost all of the attempts are based
on channeling the glycolytic flux
towards glycerol formation and on
decreasing the activities of the
pathways for dissimilation of glycerol.
 Conclusion and future prospects
-There has been a significant improvement in the microbial production of
glycerol by strain manipulation .
-The success of this commercial process was largely achieved by isolation of
yeasts able to ferment glucose to glycerol in high concentrations, yields and
productivities.
- Furthermore, improvements in the glycerol yields and productivities of yeast
strains would help to improve the economics of the glycerol fermentation
processes.
- The challenge for the biotechnologist will be to apply the fundamental
knowledge on glycerol metabolism to manipulate yeast strains appropriate for
economic glycerol production by microbial fermentation.
THANK YOU