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Protoplast Isolation N Culture and Somatic Hybrid
Protoplast Isolation N Culture and Somatic Hybrid
Outline
- Protoplast
- Sources
- Protoplast isolation
- Protoplast culture
- Environmental factors
Protoplast
PROTOPLAST
Protoplast isolation
Mechanical - Klercker(1892)
Enzymatic - Cocking(1960)
Mechanical method
pectinases
PH - 4.7 to 6.0
Pheno-safranin (0.1%).
STEPS:
Fusion of protoplasts
Selection of hybrid cells
Identification of hybrid plants
Verification and characterisation of somatic hybrids
PROTOPLAST FUSION
1972: Carlson
From protoplast fusion
N. Glauca + N. langsdorffii
(2n= 24) (2n=18)
somatic hybrid
(2n= 24)
MECHANISM OF FUSION
Morphology
Isozyme analysis
Chromosomal constitution
Genetic characterisation.
SOMATIC HYBRIDIZATION IN SOLANUM
Rice biotechnology:
Somatic hybridisation for improved
salinity tolerance and xylem
colonisation by rhizobia
for endophytic nitrogen fixation
Edward C. Cocking
Plant Science Division, School of Biological
Sciences,
University of Nottingham (U.K.), Sep:1996
Overall aim of research