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What’s PRP(Platelet Rich Plasma)?
 Agenda
• About MOOHAN

• What’s PRP?

• Effectiveness

• Effectiveness (Clinical Data)

• Application

• Introduce MOOHAN PRP Product Kit

• How to Make PRP

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 About MOOHAN
• Company Name : MOOHAN Enterprise Co., Ltd.

• Country : Republic Of KOREA

• Established : Since 1986

• Office
• Head Office : Hawolkok-Dong Office, Seoul
• Factory : Gwang-Ju, Kyeong-gi province

• President : Hyo-Seop Song

• Total Number of Employee : 50 Employees

• Business Areas
• Medical device / Food Processing Machine / Fishhook / Cosmetic
•
• Trading Nations
• USA, France, Turkey, Singapore, HongKong, Taiwan,
• Japan, China, Vietnam, Mexico, Peru, U.A.E, Oman, Saudi Arabia

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 What’s PRP
• Platelet Rich Plasma or PRP is blood plasma with concentrated platelets
• normal concentration of platelets : 200,000 per micro liter
• PRP : 1,000,000 per micro liter
• PRP consist :
• Platelet 94% / Red blood cell 5% / White blood cell 1%
• Platelet
• Heamostatic sealant
• Scaffold for tissue regeneration
• Stem cell binding
• Growth factor concentration
• Size : 2 micrometer
• Consist :
• Pseudopodial extensions
• Invaginations
• 3 types internal vesicles
• 3 types Internal vesicles(*contain inactive growth factor)
• Lysosomal vesicle
• Dense vesicle
• Alpha vesicle

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 What’s PRP
• Alpha internal vesicles
• PDGFaa, PDGFbb, PDGFab
• TGFbeta1, TGFbeta2
• VEGF
• EGF
• Action of growth factor
• Chemotaxis – directional movement in response to a
chemical stimulus
• Stem cells are attracted to growth factors and migrate into the
area
• Prolileration
• Cell division in reaponse to a stimulus
• Platelet signaling
• Growth factor binds to stem cell membrane
• Growth factor stimulates cell division

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 What’s PRP
• Charicteristics of growth factor
• PDGF
• Chemoattractive for WBCs and mesenchymal stem cells
• TGF
• Promotes cell mitosis
• Significantly increases type I
• Collagen production in tendon
• Sheath fibroblast
• VEGF
• Stimulates angiogenesis
• EGF
• Important role in the regulation of cell growth, proliferation, and
differentiation by binding to its receptor EGFR
• PDGF
• 17 ng/ml (5 fold increase over whole blood)
• TGF-B
• 42 ng/ml (3.6 fold increase over whole blood)
• VEGF
• 955 pg/ml (6 fold increase over whole blood)
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 What’s PRP
• Growth Factors Associated with Platelets
• PDGF
• Chemoattractive to Mesenchymal Stem Cells and endothelial
cells.
• Differentiation for fibroblasts and osteoblasts. Up regulate effects
of other growth factors on cells such as macrophages.
• TGF-ß
• Promote cell mitosis and differentiation for connective tissue and
bone.
• Acts on Mesenchymal Stem Cells, preosteoblasts and fibroblasts.
• Inhibits osteoclast formation
• IGF
• Mitogenetic to osteoblast lineage cells and stimulators of bone
formation from existing osteoblasts.
• VEGF
• Stimulate angiogenesis, chemoattractive for osteoblasts
• EGF
• Induce epithelial development and promote angiogenesis
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What’s PRP

Normal Platelet Count

Autologous Platelet Graft

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Introduce PRP Product Kit
1. Tubex : Tube for One Step
Separating method

2. Needle(26G×60) : The needle

to use for extracting
PPP(Pure Platelet Plasma)

3. Syringe((10cc, 23G×1) : The

syringe for drawing blood

4. Syringe(10cc, 23G×1) : The

syringe for extracting
PPP(Pure Platelet Plasma)

5. Syringe(1cc, 26G×1/2) : The

syringe for extracting
1 2 3 4 5 6 7 PRP(Platelet Rich Plasma)

6. Calcium(Distilled Water 100cc

+Ca 10g) : Additional Material
at activating

7. Anticoagulation(ACD-A) :
Anticoagulation
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Introduce PRP Product Kit
1. Selex : The
equipment to
separate the blood
2. The bucket holder
3. The balancing holder

2 3
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Introduce PRP Product Kit
1. The upper Chamber Cork :
Turn to the right side for
1 opening the cork, Turn to
the left side for closing the
cork

2. The lower Chamber : Turn to

the right side to put the
underside blood to the upper
2 area, turn to the left side to
put the underside blood to
the bottom area.

3. The Separation valve : The

valve to prevent the mixing
between the red blood cells
and the separated plasma, If
3 you close the separation
valve, hold the lower
chamber and then turn the
upper chamber to the left
side.

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Introduce PRP Product Kit
1. Tubex
2. The balancing holder
3. The Preparation before
use Selex
• As you see the picture,
when you put the Tubex
into the bucket holder,
you must put the
balancing holder as the
10cc drawn blood
weight into the opposite
1 2 side. You can run Selex
much smoothly because
you keep the balance
between Tubex weight
and the balancing
holder to prevent
shaking right side, left
side, up side and down
side.

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How To Make PRP
1. Drawing blood
• Put the 1.0 cc of
anticoagulation(ACD-
A) into the syringe of
drawn blood before
draw blood.

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How To Make PRP
1. Drawing blood
1. Draw the 8.0 cc of
blood using the
syringe containing
anticoagulation(ACD-
A).
2. Caution : You have to
draw blood more
slowly

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How To Make PRP
1 1. Drawing blood
1. After Drawing blood,
close the needle
cover
2. You have to swing
the syringe from the
left side to the right
side to mix blood with
2 anticoagulation(ACD-
A) during 4~5 times.

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How To Make PRP
2. Inject the drawn blood
into Tubex
• Disinfect the lower
chamber of Tubex
with alcohol cotton.

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How To Make PRP
2. Inject the drawn blood
into Tubex
• Inject the 9.0 cc of
drawn blood into
Tubex slowly

1 2

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How To Make PRP
1
3. Separation
• Put the Tubex
containing the 9.0cc
drawn blood into the
bucket holder and put
the balancing holder
as the 10cc drawn
blood weight into the
2 opposite side.

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How To Make PRP
3. Separation
• Close Selex's cover
and set the
separation value.

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How To Make PRP
3. Separation
1. Set RCF mode using
the mode selection
button(RPM/RCF).
2. Set 1,400 value using
up and down arow
2 above RPM/RCF
button.

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How To Make PRP
3. Separation
• Set the 4 minutes
using up and down
arow above Time
2 button.

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How To Make PRP
3. Separation
• Push SOFT SART
button to run Selex

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How To Make PRP
3. Separation
• After Selex running
finished, Selex cover
was opened with
"TTAK" sound and
then check the first
spin output.

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How To Make PRP
3. Separation
• Caution : You must
lift up Tubex vertically
without any swing. If
you need underside
blood level to adjust,
you use lower
chamber.

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How To Make PRP
3. Separation
• If you need underside
blood level to adjust,
you use lower
chamber.
• Up : Turn to the right
side
• Down : Turn to the
left side.

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How To Make PRP
3. Separation
• After check the first
spin output, you have
to close the
separation valve to
prevent the mixing
between the red
blood cells and the
separated plasma to
turn the upper
chamber to the left
side holding the lower
chamber.

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How To Make PRP
4. Concentration
• Close Selex's cover
and set the
separation value.

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How To Make PRP
4. Concentration
1. Set RCF mode using
the mode selection
button(RPM/RCF).
2. Set 2,000 value using
up and down arow
2 above RPM/RCF
button.

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How To Make PRP
4. Concentration
• Set the 6 minutes
using up and down
arow above Time
2 button.

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How To Make PRP
4. Concentration
• Push SOFT SART
button to run Selex

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How To Make PRP
4. Concentration
• After Selex running
finished, Selex cover
was opened with
"TTAK" sound and
then check the
second spin output.

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How To Make PRP
4. Concentration
• Caution : You must
lift up Tubex vertically
without any swing to
prevent the mixing.

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 How To Make PRP
5. Extraction PPP
1. Syringe(1cc,
26G×1/2) : The
syringe for extracting
PRP(Platelet Rich
Plasma)
2. Calcium(Distilled
Water 100cc +Ca
10g) : Additional
Material at activating
3. Syringe(10cc,
23G×1) : The syringe
for extracting
PPP(Pure Platelet
Plasma)
4. Tubex : The Second
1 2 3 4
Spin output

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How To Make PRP
5. Extraction PPP
• Extract PPP from
Tubex using the
syringe of
needle(23G×60)
except the 1.0 cc of
PRP.
• Caution : Extract PPP
not to swing slowly as
Tubex set to vertical.

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How To Make PRP
1 2
6. Add calcium and
Extract PRP
1. Extract calcium
2. Add the 0.1cc of
calcium(10%) into the
1.0 cc of PRP in
Tubex

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How To Make PRP
6. Add calcium and
Extract PRP
• Caution : After added
calcium, close the
upper chamber
turning to the left
side.

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How To Make PRP
1
6. Add calcium and
Extract PRP
• After added calcium,
shake the Tubex at
4~5 times.

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How To Make PRP
6. Add calcium and
Extract PRP
• Extract the 1.0 cc of
PRP from Tubex with
syringe(26G×1/2)
turning over Tubex.

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Thanks.

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