Qualitative analysis test

for carbohydrates

‫ غال محمد العتيبي‬: ‫االسم‬

515 :‫الشعبة‬
MOLISCH TEST

● Principle: carbohydrates when treated with concentrated sulfuric acid undergo

dehydration to give furfural derivatives.
● Procedure: put 2ml of carbohydrate solution in clean and dry test tube. add 2 drops of
ethanolic Alpha Naphthol (Molisch reagent) and mix, after mixing , amount of 2ml
sulfuric acid is slowly added down the sides of the sloping test tube ,without mixing to
from 2 layers.
● Inference ( results ) : A positive Molisch test indicates the appearance of a reddish –
purple or purple ring at the junction of two fluids.
● Interpretation :
Benedict’s test
● Principle: Benedict’s test is a quantitative test. The color formed depends upon the amount of
reducing sugar present in the mixture.
● Procedure: put 5 ml of Benedict’s reagent and after that add 8 drops of carbohydrate solution .
Leave to boil on the flame or in a boiling water bath for 2 min . Then let the solution cool down.
● Inference ( results ): A positive test with Benedict's reagent appears by changing its color
changes from a clear blue to a brick-red precipitate. The color formed depends on the amount of
reducing sugar in the mixture or if the test tube contains carbohydrates, this solution is orange-
red, but if it does not contain any carbohydrates, the color of this solution does not change.
● Interpretation:
Barfoed’s test

● Principle: