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Bacterial Taxonomy: Prof. Khalifa Sifaw Ghenghesh
Bacterial Taxonomy: Prof. Khalifa Sifaw Ghenghesh
BACTERIAL TAXONOMY
Feature:
• Cell shape • Cell wall constituents
• Cell size • Energy sources
• Colonial morphology • Fermentation products
• Ultrastructural • Growth temperature
characteristics optimum & range
• Staining behaviour • Osmotic tolerance
• Mechanism of motility • Oxygen relationships
• Cellular inclusions • pH optimum & growth
• Carbon & nitrogen range
sources • Sensitivity to metabolic
inhibitors & antibiotics
Adansonian or Numerical .2
Classification
• STRAIN:
• A Group of Pure Cultures Derived from a
Common Source and Thought to be the
Same.
• SPECIES:
• A Group of Closely Similar Strains.
INTRASPECIES CLASSIFICATION
• Biotypes
– Biochemical properties.
• Serotypes
– Antigenic features.
• Phage Types
– Bacteriophage susceptibility.
• Colicin Types
– Production of bacteriocins.
Nomenclature
• Naming of microorganisms.
• Governed by international rules
• Rules published in the International
Code of Nomenclature of Bacteria.
• The International Journal of Systematic
Bacteriology
Rules for the Nomenclature of
Microorganisms
• There is only one correct name for an organism.
• Names that cause error or confusion should be
rejected.
• All names in Latin or are latinized.
– The first word (genus) is always capitalized.
– The second word (species or specific epithet) is not
capitalized.
– Both genus and species name, together referred to as
species, are either underlined or italicized when appearing
in print.
– The correct name of a species or higher taxonomic
designations is determined by valid publication, legitimacy
of the name with regard to the rules of nomenclature, and
priority of publication.
Nomenclature
B. subtilis + Rod + -
C. freundii - Rod + -
E. faecium + Coccus - -
P. vulgaris - Rod + +
S. aureus + Coccus + -
• Dichotomous Key
Gram reaction
+ -
morphology indole
rods cocci + -
catalase
+ -
S. aureus E. faecium
Enterotube
• Bergey's Manual
– Methods for distinguishing and identifying
bacteria are assembled into Bergey's
Manual of Determinative Bacteriology
– Bergey's Manual of Systematic
Bacteriology
• Provides description of physical & chemical
characteristics and system of identification of
medically important members of selected
sections of bacteria
Polyphasic Bacterial Taxonomy
• More data will become available, more bacteria will be
identified, there will be more information, and software
development will need to address the combination and
linking of the different databases.