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Pemeriksaan RT-PCR Sars Cov2
Pemeriksaan RT-PCR Sars Cov2
Basti Andriyoko
Pokja Molekuler
PDS PatKLIn
orofaring swab
Safety procedures
Apus orofaring
Standard
Good laboratory practices
Safety equipment Facility design
and technique
• Control access to the laboratory • BSC • BSL
• Wash hands • Eyewash / shower
• No food / drink • Fire extinguisher
• Wear PPE risk assessment • First aid kit
• Disinfect work areas before & after use
• Dispose all solid waste material in a
biohazard bag and autoclave it
• Treat all microorganisms as potential
pathogens U.S. Department of Health and Human Services,CDC,NIH. Biosafety
• etc. in Microbiological and Biomedical Laboratories. 2009
Risk Group Classification
Containment Levels
Due to the ongoing public health
emergency, limited diagnostic work such as
non-propagative diagnostic laboratory work
(for example, sequencing, nucleic acid
amplification test [NAAT]) can be carried out
at containment level 2 (Biosafety Level 2-
BSL2).
www.hsa.ie
risk assessment
BSL 2
Sarana yg perlu diperhatikan terkait Ventilation system
SARS CoV 2: • Mechanical ventilation systems that
• BSC : Minimal Class II A provide an inward flow of air without
• Autoclave : pengolahan limbah recirculation to spaces outside the
• Untuk pengaturan udara Portable laboratory
Hepafilter
www.CDC.Gov/24-7
WHO. Laboratory biosafety manual. 2004
PCR Lab
http://grcpk.com/wp-content/uploads/2014/10/12.-Setting-up-PCR-Lab.pdf
Sarana yg perlu diperhatikan
• Saat PCR
Sampel sudah dalam bentuk RNA aman
Gunakan APD standar: Jas lab, sarung tangan, masker bedah
Alat/Perusahaan/Institusi Gen
Abbott m2000rt N, RdRp
CDC N1,N2
Cepheid GeneXpert E, N2
Roche Cobas 6800/8800 E, orf1
Allplex 2019-nCoV Seegene E, RrRp, N
nCov Real Time Detection SD Biosensor E, RdRp
Interpretasi
Cut off untuk seluruh target:
Saat interpretasi pastikan untuk melihat grafik PCR dan riwayat pasien
Hasil Interpretasi
Seluruh target gen terdeteksi Positif SARS CoV 2
Seluruh target gen tidak terdeteksi Negatif SARS CoV 2
Tidak semua target gen terdeteksi, contoh: Probable/Inconclusive
Dari 2 target hanya 1 gen yang terdeteksi
Dari 3 target hanya 1 atau 2 gen yang terdeteksi