NEW ANTICANCER AGENTS: IN VITRO AND

IN VIVO EVALUATION
KELOMPOK 1 :
ADI WARNA
WAHYU AJI
ANGGRAENI RAMADHALETA
NURIKHSAN FISTORO
CHYNTIA KOMALASARI

DEPARTEMEN KIMIA
SEKOLAH PASCASARJANA
INSTITUT PERTANIAN BOGOR
 BACKGROUND

CANCER
PATHWAYS ANTICANCER DRUGS
DEVELOPMENT
DRUGS FOR ANTICANCER
 Cytosine
 Arbinoside
 Hydroxyurea
 Cis-platinum
 Vincristine
 Vinblastine
 Paclitaxel
 Nitrosureas
 Methotrexate
 Flourourasil
 FUNCTIONAL VERSUS
NON-FUNCTIONAL ASSAYS
• Total number of cells
Fungtional • A number of colony
• Tumor volume
assay • Tumor cure rate

• Assesment of drug effect on

apoptotic pathways
Non-fungtional • Mechanisme of action
Assay
 COMPARATION OF IN VIVO-IN
VITRO
in vitro in vivo
Advantage: Advantage:
Less expensive Heterogeneity in tumor
Less time-consuming respond to drugs.
Evaluation of arge Prediction of drugs

quantities of new drugs effect in cancer patients

Detail characterization directly.
Activity at a certain
mechanisme of action
Describe IC50 and level.
pharmakokinetic
 COMPARATION OF IN VIVO-IN
VITRO
in vitro in vivo

Disdvantage: Disdvantage:
Not represent situation Need more time and
in vivo cost
 IN VITRO

WiDr Cell: Colon Cancer HeLa Cell: Servix Cancer

MCF-7 Breast Cancer

IN VIVO
 TUMOR GROWTH DELAY ASSAY (IN VIVO)
 Provides evidence for further drug evaluation in clinical trials.
 2 groups animal mode: First group treated with anticancer,
second group without treated.
 a direct measure of the drug effect on tumor growth:

tumor growth time of treated - control

 Both directly and indirectly acting anticancer agents can
reduce the tumor growth rate by inhibition of tumor cell
production, increased tumor cell death, or improved clearance
of dead and doomed cells.
 Determination of mechanisme need more detailed in vitro and
vivo experiments.
TUMOR CONTROL ASSAY (IN VIVO)
 Depends on therapeutic effect.
 Tumor-excision assay : less expensive than the tumor control
assay because no follow-up is necessary and the number of
animals required is smaller.
 tumors are excised after treatment, a single cell suspension is
prepared and cells are seeded into flasks or multi-well plates.
 Combination of new anticancer agents with potentially
curative therapy modalities, such as radiotherapy, can improve
the results compared with radiotherapy alone
 CONCLUTION

For evaluation of new anticancer agents, we advocate in vitro

and in vivo experiments with at least two or three different
tumor cell lines, applying functional nonclonogenic and, if
applicable, clonogenic assays.