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Analysis of Chronic Inflammatory Lesions of The Colon For BMMF Rep Antigen Expression and CD68 Macrophage Interactions
Analysis of Chronic Inflammatory Lesions of The Colon For BMMF Rep Antigen Expression and CD68 Macrophage Interactions
Analysis of Chronic Inflammatory Lesions of The Colon For BMMF Rep Antigen Expression and CD68 Macrophage Interactions
Supervisor:
Dr. Timo Bund
Student presenter:
Toros Taşgın
11/23/2021 1
Red Meat Consumption: Cancer Risk Factor
Purified H1MSB.1
Rep protein
densitometrical
quantification
Detection of BMMF Antigens in Colon Cancer Patient Tissue
Western Blot Analysis
Cloning
PCR
(with BMMF specific primers) Sequencing
Peritumor samples
• Macrophage-driven inflammation
Detection of ROS/RNS dependent DNA damage
Downstream chronic
inflammation marker
Detection of ROS/RNS dependent DNA damage
Exposure of Stem and Progenitor cells to ROS/RNS
Summary
1. BMMF1 antigen (Rep) was detected in the lamina propria between the colon crypts in
peritumor colon cancer tissues via WB and IHC.
2. Presence of BMMF1 DNA was verified by LMD-PCR-sequencing in the same peritumor areas.
3. Colocalization of Rep protein with interstitial macrophages was shown and quantified via
fluorescence IHC. Elevated levels of Rep+/CD68+ inflammatory macrophages was detected
in peritumor samples in comparison to the cancer-free patients.
4. Association of Rep protein with chronic inflammation was further verified by the detection
of ROS/RNS dependent DNA damage marker in areas with increased concentration of Rep
protein.