AGRANULOCYTES

It is a form of WBCs or Leukocytes

• The WBCs or Leukocytes which do not have
granules in cytoplasm is called Agranulocytes
• Main Agranulocytes are
• Monocytes
• Lymphocytes
MONOPOIESIS
• The process which involve in formation of
monocytes is called monopoisis  
• Monocytes are formed mainly in the bone marrow
• and migrate to the spleen, lymphoid and other
tissues
• and organs of the body where these are
transformed into macrophages.
• Various stages in its development are: 
1 . Monoblast
• It is the earliest recognizable cell of the series
• It is a large cell similar in structure to the
myeloblast
• Nuclear outline is, however, not as regular as
in myeloblast and may show indentation or
convolution
2. Promonocyte:
• It is a large cell about 20 um in diameter
• It has abundant cytoplasm, grey blue in
colour
• It may contain fine azurophilic granules
• The nucleus is usually round or kidney shaped
giving folded appearance but may be
lobulated.
3.Monocyte
• It is slightly smaller than promonocyte
• Other features are similar
• Its cytoplasm has typical ground glass
appearance
• The nucleus is like a band folded upon itself to
assume a spherical shape
LYMPHOPOIESIS
• The process which involve in formation of
lymphocytes is called lymphopoises
• Mature lymphocytes develop mainly in the
lymphoid tissues of the body
• Namely lymph nodes, spleen, gastrointestinal
tract and tonsils
• Bone marrow makes only a small contribution
to lymphocyte production.
• CFU-L probably migrates to lymphoid tissue
early in life.
• These also develop through stages
• The maturation of lymphocytes is
characterized by
• Maturation of nucleus and cytoplasm
• Adaptation to their function by expression of
specific proteins
1 Lymphoblast
• It is the earliest recognizable cell of the
series
• It measures 15-20 pm in diameter
• It contains a large, round or oval nucleus
• Nucleoli are present, usually 1-2 in number
• The cytoplasm is non-granular and deep blue in
colour forming a narrow rim around the nucleus
2. Prolymphocyte
• It is the next stage formation of lymphocyte
• Nucleus contains prominent nucleolus, usually
centrally placed
• Cytoplasm is variable
3. Large lymphocyte
• It is about 12-16 um in diameter
• Cytoplasm is sky blue in colour and contains
few granules
• which stain purplish red
• The nucleus is round or slightly indented
• Nucleoli are absent
4. Small lymphocyte
• The large lymphocyte matures into small
lymphocyte
• It is 9-12 um in diameter
• The cytoplasm is scanty and stains blue
• Purplish red granules may be present
• The nucleus is round or slightly indented
• Nucleoli are absent.
 THROMBOPOIESIS

• The process by which platelets or thrombocytes

are formed is called THROMBOPOIESIS
• Platelets are formed from the cytoplasm of a
large cell in the bone marrow known as
megakaryocyte
• This also passes through various stages of
development in the bone marrow
• These are:
1. Megakaryoblast
• It is a large cell about 20-30 um in diameter
• It has a large oval or indented nucleus that
contains several nucleoli
• The cytoplasm is blue, small in amount and
contains no granules
• It may show budding
2.Promegakaryocyte
• It is formed from the megakaryoblast
• It is larger than the megakaryoblast
• It has deep blue cytoplasm that contains
azurophilic granules
• The nucleus is non-lobulated or partly lobulated
• From here onward only the nucleus while the
cell enlarges without division (Endomitosis).
3. Megakaryocyte
• It is a large cell, from 30-90 um in diameter
• It contains a single multi lobulated or indented
nucleus
• The number of nuclear lobes varies from 4-16
depending upon the number of divisions it
undergone
• The cytoplasm is abundant and stains light blue
• It contains fine azurophilic granules
• The margin is irregular and may show
pseudopod formation
4. Platelet
• It is a small discoid structure, 1-2 um in size
• These are formed by partitioning of
cytoplasm of megakaryocyte into numerous
structures that separate to form platelets