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Meat Ingredients During Processing: Chapter Four
Meat Ingredients During Processing: Chapter Four
Processing
Chapter Four
1
Introduction:
• Meat and Muscle
Meat is defined as the flesh of animals
used as food. In practice this definition is
restricted to a few dozen of the 3000
mammalian species. It is often widened to
include, as well as the musculature, organs
such as; liver, kidney, brain,….
2
Conversion of Muscle to Meat
• The animal structure we are interested in is
made of muscles.
• These muscles are the source of our meat.
• Muscle will be converted into meat through
glycolysis (consuming ATP) during the post-
mortem process; thus the major criteria of meat,
namely; “Rigor Mortis”
• Converting muscle to meat progress (post-
mortem) can be detected through measuring the
ATP-PH correlation. 3
PH as a Parameter of Muscle Conversion
Meat
• In a living animal the PH and ATP level is 7.2 and
around100%, respectively.
• When PH became 6.3, the ATP amount is around
87%.
• Just 80% of ATP left when PH measurement is 6.0
• ATP is continuing in depletion until the PH
reached to 5.6-5.7 to indicate that there is no
more than 10% of ATP has been left.
4
5
6
Meat Manufacturing
• Meat can be manufactured into many types of
products;
– Refrigerated primal cuts, and comminuted
– Frozen primal cuts, and comminuted
– Reformed products
– Emulsified products
• Regardless meat products, there are two
major ingredients:
– Raw materials
– Non meat ingredients 7
Raw Materials
Beef:
• rounds
• corned beef, Pastrami, roast beef
• trimmings - higher quality cuts, sausage & ground
beef
• advanced meat recovery tissue
• soft separated
• partially defatted tissue
8
Poultry:
• Whole breasts (white meat) - uncured roasts
• Thigh meat (dark meat) - cured Turkey
• Mechanically separated poultry:
– lower cost, cooked sausage
9
10
11
12
Storage of Meats
• fresh/unfrozen
• < 7 days @ 28 - 30F (<40F)
• preblended (cured)
• <72 hrs @ 36F
• frozen
• 0-20F & barrier films
• Frozen How, When, & Why?
13
Frozen Meat Quality:
• Storage conditions (#1)
• Freezing method
• slow = more purge & dark color
14
Slow Freezing Causes Extra-
Cellular Water to Freeze and
Cause Cellular Damage
15
Frozen Storage Cont’d:
• Condition of meat when frozen
• rancidity & bacteria
• refreezing
• Length of storage
• gradual loss of WHC over time
16
Raw Material Parameters for Least-
Cost:
• Bind
• protein quantity & quality
• Color
• Moisture & Fat Contents
• Cost
• Availability
17
Non-Meat Ingredients
Water
• product temperature control (cold/ice)
• extract proteins
• dissolve and disperse dry ingredients
• lower product costs
– compensates for cooking losses
18
Salt
• brine strength for extracting proteins
• 7% ideal
• % salt / % salt + % water
• flavor & shelf life
• purity (rock salt vs. high purity evap. salt) not a
factor in cooking yields of injected hams.
19
Relationship Between Cooking Yield and
Salt Content
110
100
Cooking Loss, %
90
1.3% Salt
2.1% Salt
80 2.9% Salt
3.7% Salt
70
132 146 162 179
Cooked Temperature, degrees Fahrenheit 20
Phosphates
• increase pH
• increase WHC
• antioxidant - chelates cations (Fe++, Ca++, etc.)
• Na+ and K+ salts - 0.5%
• soapy flavors & color problems
21
Phosphate Types
• tetraNa+pyro - v. alkaline, but hard to dissolve
• Na+tripoly - alkaline
• Na+hexameta - neutral
• tripoly & hexameta- brines
• Na+acid pyro- cured color
22
23
24
Sodium nitrite
• cured color and flavor
• pro- and antioxidant
• prevents botulism
• restricted to 120 ppm (According to PSI)
• reaction faster with acidic & reducing conditions,
& absence of air
25
Left: Meat
chopped
without nitrite
Right: Meat
chopped with
nitrite
26
Sodium Erythorbate
• cure accelerator, reducing agent
• sodium ascorbate has same activity and can be
labelled as “Vitamin C added.”
• required in bacon (550 ppm)
Curing (addition of 156 ppm sodium nitrite) forms
mononitrosylhemochrome, the cured meat pigment (slice on
the right), vs uncured pork (left slice).
Panelists could detect a color difference in cooked
turkey breast containing 0 and 2 ppm sodium nitrite,
respectively.
Antioxidants
• butylated hydroxyanisole
• butylated hydroxytoluene
• tertiary butyl hydroquinone (TBHQ)
• propyl gallate, malic acid
• 0.01% fat - fresh sausage
• 0.03% fat - dry sausage
Antioxidants
• phosphates
• nitrite
• cloves, cinnamon, sage, allspice, nutmeg,
mace, rosemary
Sweeteners
• Sucrose (cane or beet) - non-browning, but will
caramelize.
• Dried plums
– texture & juiciness enhancer, natural,
antimicrobial, sweetener, fat/meat
replacer
Non-Meat Proteins
• Soy isolate
– 90% protein - 2% restriction
– salt is detrimental
• soy concentrate
– 70-90% protein
– flavor problems
• MSBeef
– bone particle size
Acidulants
• Lactic acid & glucona delta lactone (GDL)
– simulates fermentation
• Potassium sorbate
– 10% solution for soaking casings
45
Introduction:
• Freezing maintains meat quality for much
longer period of time than chilling.
• Freezing process must be rapid and it doesn’t
kill significant no. of M.Os. Meat of poor
microbial quality on freezing will stay poor on
thawing.
46
• Factors affect the total freezing time:
– Freezing rate.
– Frozen distance/time (i.e. 3cm/ 20 min).
• The freezing rate effect ice crystals location and
size.
• Types of freezing:
1.Fast freezing:-
– Location became wider outside & inside cells.
– Homogeneous distribution of ice crystals.
– Small ice crystals.
47
2. Low freezing:-
– Larg ice crystals.
– First outside meat cells then inside.
– Toughness cause dripping cause chewiness.
by
regulating
H2O migration
↓ evaporation losses.
↓ shrinkage. 49
• Notes:
To have the best out of packaging;
packages should be properly used and storage
temp. at correct set.
50
• Fresh meat under normal package, the
dominant microflora will be.
– Pseudomonas 70-80%
– Achromobacter ≈10% aerobic
– Acintobactor ≈10%
51
• Fresh meat under vacuum packages will have
˂1% O2 and 20-40% CO2, which in turn will
affect the growth of the aerobic M.Os.
• Vacuum packaging used for fresh & processed
meat will have CO2.
• CO2 inhibit aerobic M.Os growth by providing
slow growth, while the facultative lactic acid
bacteria shows lower growth.
52
Spoilage indicator:
•Slime formation and
•Off odors
107
No. of M.Os
105
103
0 3 7
Days Spoilage indicators are not seen
53
3. Modified atmospheric packaging:
• Only for retail cuts(pieces). Air is replaced by
gas(single or mixture).
• There are 3 common gases:
1)N2 2) O2 3) CO2
Place Flushing
Box Covering Packaged
meat with gas
formation the box product
pieces
54
Functional MAP:
• To maintain the bright red color of meat.
• Inhibiting the microbial growth through the
presence of CO2 (10-20%).
55
• High level of CO2 causes:
1.Effect meat color dark color.
2.Increase dripping; since CO2 will decrease pH,
through producing bicarobonate after
dissolving in meat juice.
3.The anerobic medium is favorite for poisoning
M.O. (e.g. Clostridium & Entero).
56
Advantages of MAP:
1. Extension the shelf-life by 60-100% by:
– Slow microbial spoilage.
– Maintain attractive color of meat.
– Slow chemical spoilage.
– Reduce drip loss.
2. Allow hygienic handling of the product on
opening.
3. Improves visibility & appearance of meat.
57
Meat storage:-
1. Chill storage:
The temp around -1.5 ◦C and the relative
humidity should be 85-90%. The storage time
depends on the original microbial quality of
meat.
2. Frozen storage:
Long preservation period for meat. Stores
designed to keep maintain the temp. of the
frozen meat.
58
Meat Expected shelf-life
at -18 ◦C
Beef 8 -12 Month
Poultry 6 -8 Month
Pork 4 Month
59
Frozen storage:
1.All microbial activities stop @ -10 0C.
2.Chemical activities continue enzymaticaly.
3.Shelf-life determining factor would be fat
rancidity.
• The advantages of temp below the freezing
point in prolonging the useful storage life of
meat and in discouraging microbial & chemical
changes tend to be offset by exudation of fluid
“ drip” on thawing.
60
• The nutritional value will be very low since
protein, peptides, a.a., and vitamin will be among
the many constituent of drip fluid.
• In freezing, one of the most important problem
was freeze burn.
Freeze burning:
• The phenomenon involves the formation of a
condensed layer of muscular tissue near the
surface. This prevents access of water from
below thus enhancing surface desiccation.
61
• Freezer burn is maximum during storage when
the meat has been frozen rapidly under
condition where evaporation has been
prevented and conversely.
• Freezer burn usually happened when the pack is
been damaged or lost or even permeable pack.
• Freeze burn happened due to the evaporation of
moisture from meat surface and it can be:
1. Severe: Irreversible at it effects on eating quality
(tough meat, chewing meat, low juiciness).
2. Mild: Reversible on thawing, just happened on the
very surface area.
62
Prevention of freeze burn:
1. Prevent drying out by effective packaging.
2. Prevent damage to packages.
3. Low air velocity is better on stores.
65
Chilling Ready-to-Eat Meat
Products
(Stablization)
Benefits of Proper Cooling:
• reduce pathogen growth
• prevent toxin production with spore formers
• comply with FSIS Appendix B
• improve peelability, product appearance, yields.
Cooling Methods:
• Air chilling
• holding coolers
• blast chillers
• Intensive chilling
• Liquid chilling
• water, brine, glycol
• Cryogenics
Holding Cooler
Air Blast Chiller
“Intensive” Air Chiller
Liquid Chiller
Liquid Brine Chiller
Cooling Process
• Heat Transfer
• Conduction & Convection
• Thermal Properties
• Product Geometry
• Thermal Conductivity
• Surface to Core Temperature Difference
Thermal Conductivity
• Controls rate of heat transfer
• (Btu / Hr-Ft ºF)
• Influenced by moisture content
• Water is a good conductor
• Protein and fat are not
Thermal Conductivity of
Meat Products
Water Thermal
Content Conductivity, k
(%) (Btu/hr-ftoF)
Franks 60 0.31
Turkey 49 0.28
68 0.29
Heat Transfer Coefficients
80
Major Problems:
• None compliance with raw material description.
• Natural contamination & foreign bodies.
• Microbiological problem.
• Chemical problem.
• Poor packaging standard.
• Poor chilling condition.
• Poor freezing condition.
• Poor storage condition.
• Poor transportation condition.
81
First: None compliance with raw material description.
82
B. Problems caused by poor butchery:
– Meat with bone fragments.
– Meat with metal pieces.
– Meat with hocks, legs, shoulders.
– Meat with excessive connective tissue.
83
• The most common type of meat is the diced one.
However, this diced meats size could effect,
cooking, properties, texture, appearance.
• During dicing meat the following should be
controlled by:
A.Temperature: since;
– Very low temp. shatter the meat.
cause
cause
myoglobin to O2 available
metmyoglobin,
as illustrated next page. 85
+ O2
Myoglobin Oxymyoglobin
Reddish - O2 Purple
Ox
ida
tio
+ NO Re n Oxidation
du
ctio
n
Oxidation
Nitrosomyoglobin Metmyoglobin
Reduction
Dark red Brown dark
Δ Δ
Oxidation
Nitrosomyochromagen Denaturated
Reduction
Metmyoglobin
Cooked red color Brown color 86
H2O2 will react with meat component like sulfur,
2
which will produce pigment providing green color.
Safety (Pathogens)
Color & ordors.
Sensational properties.
90
Fourth: Chemical problems:
• Rancid fat: it can cause:
– Browning of product.
– Objectional odor & flavor.
– Shortening shelf life.
Q. How we can determine a product shelf life?
1.Identifying the products, for example: mortadella.
2.mortadella shelf life determined according to:
93
• Mixed Meat Species:
Meat from different species of animals has been
partially or totally substituted for actual meat
species requested. Usually easy to detect different
types from each others, but veal and pork is not.
• Species Identification:
It is important to identify species in order to:
1.Help detect unfit meat and prevent it from being
sold for human consumption.
2.To prevent substitution of expensive meat with
cheap one. Good examples are: 94
• Veal with pork.
• Beef with horse.
95
Mechanically Deboned Meat (MDM):
After boning, a considerable amounts of meat
remains on bones, this meat could be separated
from bones by mechanical ways.
• Mechanical separator: This involves 2 steps:
– Mincing bone and meat.
– Separation of meat and bone from each other.
96
Factors that may affect composition of MDM:
• Age of bones.
• Types of bones.
• Temp. of recovery.
• Feed rate of the machine.
• Amount of meat on bones.
• Types of machine used.
97
Important specification of MDM:
• Chemical:
• H2O content.
• Fat content.
• Ash
• Max bone content.
• Protein, pH, Peroxidase.
• Fat: free fatty acid(F.F.A).
• M.C (Microbial counts). End of Chapter Six
98
Emulsion
Chapter Seven
99
Definition :
102
Heat break down:
• Fat separation may arise as a result of heating too
rapidly or at too high temp.
• During heating:
1- Fat particle expands.
2-Protein coating has tendency to shrink (outer protein).
3-Inner most protein expands.
• *Coagulated protein sac ruptured.
• *Entrapped fat separates or renders.
103
• Casing:
*Casing needed for most sausage.
*Casing give size and shape.
*Cases must be strong to hold the meat, but have
shrink and stretch characteristic.
The forces that affect casing are:
1- forces produced during stuffing.
2- forces of linking or closure.
Casing for sausage are obtained from two basic
material:
1- Cellulose.
2-Collagen. 104
That present in:
– Cloth
– Regenerated collagen.
– Animal.
– Cellulosic casing.
105
Clips and thread closure:
*It used for closing sausage casing.
*The main source of thread is cotton.
*There is two measure type of thread (2 to 16 ply).
2 ply → frankfurter and small sausage.
12-16 ply→ bologna, salami, and other large sausage.
106
Additives:
• The processing, handling and storage of
sausage for today’s market has required the
use of additives to meet the demands of
modern consumers.
• The food chemical codex is a good reference
for the quality requirements of these items,
and in Palestine we use PSI.
107
Ice or water:-
*Ice or water are added to meat to provide
considerable functional qualities.
*Make a chilling for meat that make a lowering the
initial temp. and by lubricating the meat mass.
*Added water aid in dissolving sodium chloride and
curing salt to make more distribution in the mass.
* Water imparts fluidity to the emulsion in stuffing
and to give good shape in the cases.
* Texture and tenderness of the finished sausage are
markedly affected by the added water.
108
• Salt:-
*Salt for sausage must be of food-grade quality.
*There are three functions of NaCl:
1- It dissolves in water to form a brine which acts to
retard microbial growth.
2-It aids in solubilizing the myosin-type protein of
comminuted muscle for emulsifying the fat in
emulsion sausage.
3-It contributes the basic taste characteristics.
109
Curing salts:-
*The main concept of curing salts is for preservation.
*No more than 200 ppm sodium nitrite will be
present in the finished product.
*Small quantity of nitrite in combination with:
1- moistur level,
2-pH,
3-Added salt,
4- Final internal processing temp,
5-general bacterio static,
6-flavour.
110
Ascorbates:-
*Ascorbates and erythrobates are closely related
chemicals that can be used inter changeably in
sausage mixes.
*It is active reducing agent that react with Nitrite to
give Nitric Oxide.
*Ascorbate is better than erythrobate due to:
A very specific action of ascorbic acid or its salt
has been noted in relation to limiting the formation
of green discoloration that occur at times.
111
Sugar:-
Adjunct to provide flavor:-
*Mask salt flavor.
*Provide a reservoir for an acid forming substance.
112
Phosphate:-
*It is used principally to accelerate development
of cured color in frankfurters in a continuous
cooker.
*The percent in the finished product is 0.5%.
113
Acids:
*It is using especially for small sausage.
*Acid is sprayed prior smoking or cooking.
*Aim of acid using:
1- Reduce surface pH of sausage.
2-Coagulates proteins at the surface.
3-Permits coagulation at lower temp.
4-Spraying acid gives better surface and help
development of surface color.
117
118
119
120
Meat Emulsions
• true emulsion or batter?
Stable batter or matrix,
based upon:
• viscosity & gelation potential
• quantity & type of soluble
protein
• cooking binds water/trap fat
• less functional ingredients
• fat, C.T., rework, extenders
To form a stable meat
batter:
• start by maximizing functionality of lean meats
136
137
138
139
Filler
140
End of Chapter Seven
141
SPICES AND
SEASONINGS
Chapter Eight
Spices and Seasonings
• Spices
–natural plant parts
• Seasonings and Flavorings
–mixtures of spices
–natural?
Taste
Regions of the
tongue
– sweet – tip
– salty – front sides
– sour – rear sides
– bitter – across rear
Balance of Taste
Sensations and Flavors
• Maximum – distracting
Sensory Descriptions
• Coriander - like eating rose
petals
• Chili - burnt flavor
• Cumin – dirty sock
• Fennel – licorice flavor
• Nutmeg – eggnog
Sensory Descriptions
• Sage – Vicks Vaporub
• Oregano – freshly cut hay
• Paprika – corn silage
high levels – tomato
• Cloves – numbs lips/tongue
Meat Product Spice
Characteristics
e.g., Smoked sausage: a "simply" seasoned
product, with black pepper as the major
spice flavor, red pepper is used at very
low levels, coriander is used sometimes.
Smoke and the sweet burnt flavor of
dextrose are the major flavors of smoked
sausage.
End of Chapter Eight
Smoking and Thermal
Processing Meat Products
Chapter Nine
151
Heat Transfer
• Convection
– heat transfer resulting from mixing of air or liquids
at different temperatures
• Conduction
– heat transfer from surface to center of products
– e.g., nail in potatoes
• Radiation
– wave transmission through space
– microwave heating
152
TD = Temperature
Difference
Product Cross-section
Heat Transfer:
Driving force for heat transfer is the
surface-to-core temperature difference
153
Product
Shape
155
Natural Smoke Deposition
Depends Upon:
• type & condition of wood (chips, sawdust, or
logs)
• logs – more smoke, pink ring
• %RH
• Smoking (contact) time
• Air temp. & velocity
156
Pink Ring on BBQ Beef
157
Naturally Smoking Loop Sausage
158
Liquid Smoke
• environmentally friendly
• natural smoke is captured in acidic solution
• atomized or dipped
• now very close to natural smoke taste/aroma
159
Thermal Process Troubleshooting
160
Regulatory
Requirements and
Product Safety
161
Meat Inspection
Requirements:
• Hairnets required to cover hair on head, beards &
mustaches (ODA)
– Have the “right to bare arms.”
162
• No jewelry (rings, watches, etc.)
• Separation of raw vs. cooked, fresh vs. cured
products
• Label product:
163
• Keep cardboard boxes off tables, other contact surfaces
164
Fully Cooked Products
• Contact surfaces and gloved hands cleaned &
sanitized.
• Also need clean knives, thermometers, blue
coats.
• Separation of products in time and space.
165
5 Parts of SOP:
• Must have a written plan describing daily
procedures that establishment will conduct before
and during operations, and frequency at which they
will be conducted to prevent direct contamination
or adulteration of product (s).
166
SOP Cont…..
• Signed/dated by person with on-site authority upon
initiation & when modified.
167
General Lab Practices
• Need to contact Lab officer, before doing
any work in Meat Lab.
• Clean up after labs and individual project
processing.
• Dress warmly for labs.
168
Processed Product
Safety
169
Processed Meat Microbiology: Lines
of Defense
• minimize contamination
– raw materials & finished
• processing interventions
– salt, nitrite, lactate, cooking
• suppress growth
– packaging, temp. control
170
Where Contamination Can
Happen?
• plant design & construction
• equipment design
• raw materials
• maintenance
• employee hygiene
• cleaning & sanitizing
171
Processing Interventions
• Salt, nitrite, lactacte, phosphates
• floral shift Gram- to Gram+
– lag phase after curing
172
Processing Interventions
• Cooking
• 144 to 160F (140F for 12 min)
• 95% reduction
• more susceptible to contamination
173
Processing Interventions
• Cooling (stabilization)
• slow C. perfringen growth
• Freezing
• slows growth, doesn’t always destroy organisms
• Irradiation
• beef, poultry & spices, not processed meat products.
174
Hurdle Effect or Barrier Concept
• synergism using 2 or more intervention
methods.
• multiple hurdles decrease the chance of
resistant strains.
175
Pathogens vs. Spoilage
Organisms
• spoilage organisms cause color, aroma and
flavor changes
• pathogens cause illness and sometimes death
• pathogens not competitive
176
Spoilage:
• Quality changes due to m.o. growth and
byproducts of growth
• oxidation of color/fat, flavor changes
177
178
Food Intoxication:
• toxin ingested
• short incubation period
• no fever
• Staph. poisoning & botulism
179
Food Infection
• bacteria ingested
– toxin produced in stomach
• longer incubation/fever
• Salmonella, E. coli, Campylobacter
180
What organisms should the meat industry
be most concerned about?
• E.coli 0157:H7?
• Salmonella - 24 hr. Flu?
• Campylobacter?
181
Food-Borne Illness Cases in US:
• processing plants - 5%
• home preparation - 20%
• away-from-home prep - 75%
182
Listeria Interventions:
Processing
183
Processing Interventions
• Product Formulations
• Post-Packaging Pasteurization
184
Product Formulations
• Some products will not support growth of
L. monocytogenes:
• High acid content (< pH4.5)
» Fermented products (lactics)
• Low water activity
• High salt levels
185
Product Formulations
• Organic Acids
• Smoke – liquid or natural
• Spices – natural resins.
• Preservatives
• Bacteriocins
186
Organic Acids
• 4.8% sodium and/or potassium lactate and
• 0.25% sodium diacetate
approved as antimicrobial agents in meat products.
187
Organic Acids
• Effective when added to formulations, but not as
a dip.
• 2-3% sodium lactate without flavor problems.
• >0.12% sodium diacetate can result in vinegar
flavor.
188
Smoked Products
189
Preservatives
• High levels of sodium benzoate, propyl
paraben, potassium sorbate.
• More effective at lower pH and temperatures
• BHA, BHT, TBHQ effective.
• Limited approval for use in meat products.
190
Bacteriocins
• Natural polypeptides from bacteria that inhibit
other bacteria.
191
Post-Packaging Heat/Pasteurization
• Heat re-applied to package surfaces to destroy
any surface cross contaminated.
• Immerse package in hot water
• Surface steam (205F, 15 sec)
• Hot air or infrared.
192
Post-Packaging Heat Disadvantages
• Difficult to heat all surfaces
• May adversely affect package.
• Product changes with heat (color, texture, purge, etc.)
• Expensive
• Apply heat/moisture in ref. room (condensation, etc.)
193
Non-Thermal Post-Packaging
Treatments
• High Pressure (HPP)
– Expensive, batch process
• Pulsed Electric Field (PEF)
– better with fluid products
• Ultraviolet (UV) Light – Lm resistant, surface effect only
• Electrolyzed oxidizing water
– some promise, not near term
194