Week 8 L2 Plant Immunity and CRISPR 2

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Plant Immunity

RNA Interference

CRISPR (Acquired Immunity in bacteria)


Pivoting the Plant Immune System from Dissection to
Deployment

by Jeffery L. Dangl, Diana M. Horvath, and Brian J. Staskawicz

Science
Volume 341(6147):746-751
August 16, 2013

Published by AAAS
“Sir Rowland Biffen identified a single recessive gene for resistance
to wheat yellow rust caused by P. striiformis.”

Jeffery L. Dangl et al. Science 2013;341:746-751


Pathogen virulence
Disease resistance R Genes “effector gene” in the
in the Plant pathogen or herbivore (virus,
fungus, insect bacterium.)
hypersensitive response : programmed cell death
Jeffery L. Dangl et al. Science 2013;341:746-751
Fig. 2 Hawaiian papaya plot in 2011.

Jeffery L. Dangl et al. Science 2013;341:746-751

Published by AAAS
RNAi (RNA interference)

Systemic Acquired Resistance

http://robustplants.org/plant-health/Systemic-Acquired-Resistance-Plants-Secret-Weapon
Plant Immunity

RNA Interference

CRISPR (Acquired Immunity in bacteria)


Plant Immunity

RNA Interference

CRISPR (Acquired Immunity in bacteria)


Genome Editing - CRISPER

clustered, regularly interspaced short palindromic repeats


Genome Editing - CRISPER
Genome Editing - CRISPER
Genome Editing - CRISPER
Genome Editing - CRISPER
Genome Editing - CRISPER
Genome Editing - CRISPER

http://www.ozbiosciences.com/content/58-transfection-reagents-for-genome-editing
Genome Editing - CRISPER

www.labome.com

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