CBIO-60

LAB
Test for
Carbohydrates
(Experiment 6)
Maricar P. Dimas, LPT
 Table of contents
Types of Reaction and Mechanisms Involved
in Carbohydrates
Carbohydrates
01 Diferent reactions of 02 How are carbohydrates broken
carbohydrates in specific test down and formed?

Reducing vs. Non- Test for

Reducing Carbohydrates Carbohydrates
03 04 Molischs , Bials and
Seliwanoffs Test
01
Types of
Carbohydrates
 Physics
You can give a brief description of the topic you
want to talk about here. If you want to talk about
Mercury, you can say that it’s the smallest planet
in the Solar System
 Types of Carbohydrates
Do you know what helps you make your point
clear? Lists like this one:

● They’re simple
● You can organize your ideas clearly
● You’ll never forget to buy milk!

And the most important thing: the audience won’t

miss the point of your presentation
 02
Reactions of
Carbohydrates
General Reactions Involved in Carbohydrates

Mars is actually a
very cold place

Dehydration
The Earth is the
planet we live on

Hydrolysis
Jupiter is the biggest
planet
03
Reducing vs.
Non-Reducing Sugars
Definiton and Differences
 04
Test for
Carbohydrates
You can enter a subtitle here if you need it
 73 63
% %
South Korea Malaysia
Mars is actually a Saturn is a gas giant
very cold place and has rings
John Do Ann Smith
 Summary
Test Defintion Reagents used Principle Result
A biochemical test used to α-naphthol
detect the presence of CHO in
general dissolved in
Molisch’s Test ethanol and
Purple Violet-ring
Hans Molischs H2SO4
Complex
A biochemical test used to test
pentoses and pentosans and to Orcinol,
detect presence of RNA concentrated
Bial’s Test
HCl and ferric Blue Green
Manfred Bial chloride Color

A biochemical test used to

Seliwanoff’s Test detect and distuinguish ketose Mixture of
sugar (with at leat 5-C
molecule from Aldose sugar
resorcinol and
HCl Cherry Red
Theodore Seliwanoff Complex
 Result
Sample CHO Category No. of Carbons
Molischs Bial’s Test Seliwanoff’s Test
(general test for (pentoses) (ketose)
CHO)
1.Glucose Monosaccharide – 6-hexose + - -
Aldose

2. Starch Polysaccharide – Large number + - -

glucose monomer -
aldose
3. Xylose Monosaccharide- 5-pentose + + -
aldose
4. Lactose Disaccharide - Aldose 12-hexose + - -

5. Fructose Monosaccharide - 5-pentose + + +

Ketose

6. Ribose Monosaccharide - 5-pentose + + -

Aldose