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2020 Mikdas Pert-5 Teknik Kultivasi Bakteri-1
2020 Mikdas Pert-5 Teknik Kultivasi Bakteri-1
Rate of
growth
Chemical
-10 0 10 20 30 40 50 60 70 80 90 100110
Temperature in ºC
• Carbon source
• Nitrogen, sulfur phosphorus
• Oxygen
Lyophilization
• Freeze dry process that protects bacteria
A. Glass jar
Candle
concentration of CO
e.g. Brucella abortus
Gas generator
Petri plates
Gas generator
Laboratory Training for Field EEpidemiologists
P I D E M I C A L E R T A N D R E S P O N S E
Culture methods
Streak culture Stroke culture
• Isolation of bacteria in • To obtain pure growth for
pure culture from clinical slide agglutination;
specimen biochemical tests
Lawn culture Stab culture
• Antimicrobial • Maintenance of stock
susceptibility testing (disc cultures
diffusion), bacteriophage Pour-plate culture
typing
• Quantification of bacteria
Liquid cultures in liquid cultures, urine
sample
Lag
phase
0 5 10
Time (hr)
of the bacterial
Explosiveness of exponential growth
growth curve
• Short generation time: small number of bacteria initiate a dangerous
illness (e.g. acute meningococcal meningitis).
• Long generation time: tuberculosis bacillus causes chronic illness
Inside body tissues
• Bacteria are stressed
• Bacterial populations are rarely fully viable
• May cease growth but continue synthetic activities to meet adaptive
stress
Non-growing bacteria can also be harmful:
• Immunogenic
• Production of toxins starts or accelerates during stationery phase
• Sporulation can release toxins
Effectiveness of antimicrobials
• Number of organisms – larger number longer to eliminate
• Environmental factors – organic materials reduce effectiveness
• Timing of exposure
Quantitative methods
• Give accurate estimate of bacterial number; more exact
applications
• Vaccine production
Institut Pasteur