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Enzyme Kinetics
Enzyme Kinetics
Enzyme Kinetics
Enzyme Kinetics
S.M.K
10
10//2010
=
Enzymes background
Definition
Important prosperities
Enzyme Kinetics
Michaelis--Menten equation
Michaelis
Line weaver-
weaver-Burk plot
Practical exercise
Enzymes background
Enzyme definition
Enzymes are the catalysts of biological systems
and are extremely efficient and specific as catalysts.
Prosperities of Enzymes
Accelerates the rate of the reaction
Highly specific
Enzyme accelerates the of a reaction
Enzyme accelerates the rate of a reaction by factors of at least a million compared to
the rate of the same reaction in the absence of the enzyme without affecting the
equilibrium.
Enzymes do not alter the equilibrium point of the reaction. This means that the
enzyme accelerates the forward and reverse reaction by precisely the same factor.
åor exampleü consider the interconversion of A and B.
AȼB
Suppose that in the absence of the enzyme the forward rate constant (kf) is 10-4
s-1 and the reverse rate constant (kr) is 10-6 s-1. The equilibrium constant (Keq) is
given by the ratio of the two rate constants.
There are two proposed models to explain the specificity of the interaction
between the substrate molecule and the active site of an enzyme.
(a) The ´lock and keyµ model ² in this model the substrate has a shape
matching the enzyme·s active site
(b) The ´induced fit modelµ ² the active site has a shape
complementary to that of the substrate ½ ½
Enzyme kinetics
Gefinition
It is the branch of enzymology that deals with the factors
affecting the rates of enzyme-
enzyme-catalyzed reactions.
Vedio (1) (2
(2)
This curve is biphasic
Phase 1: (1st order kinetics)