COLUMN

CHROMATOGRAPHY
GROUP 1
Agbayani, Mark Kevil
Ahuyan, Eugine P.
Bojno, Nadia B.
Bulayang, Hanz Christian A.
Capua, Russel A. Jr.
INTRODUCTION

 Chromatography is purification technique. Individual components

are separated from a mixture by passing it in solution or suspension
through a medium in which the components move at different rates.
In case of column chromatography the separation occurs with help
of a column, therefore it is termed as column chromatography.
 Column is a tube like structure made of glass or plastic.
PRINCIPLE

 The main principle involve is absorption of solutes through a

stationary phase and separates the mixture into individual
components.
 The mostly used stationary phases are salica and alumina. Mobile
phase may be single phase solvent or mixture mobile phase is
opposite in nature to stationary phase.
COLUMN CHROMATOGRAPHY
 It is a most widely used chromatography which finds daily use in
research labs and industries. It is easy to operate and less expensive
technique. As the name indicates, there is a long column which is
suspended in the air with the help of stand.
 Into the column, the stationary phase is the packed and the mobile
phase is allowed to flow taking the sample through the solid
column.
INSTRUMENTATION OF COLUMN
CHROMATOGRAPHY
 Mobile Phase - Liquid
 Stationary Phase - Solid
PREPARATION OF COLUMN
 The column mostly made of glass tube packed with stationary phase
either salica or alumina.
 A glass woll or cotton is placed at the bottom of the column before
packing the stationary phase.
Two types of preparing the column:
 Dry Packing or Dry Filling
- The Dry Powder is filled in the column and then the solvent is
allowed to flow through the column.
 Wet Packing
- The slurry of absorbent with the mobile phase is prepared and is
poured into the column stationary phase should be filled uniformly
without any air bubbles.
ADVANTAGES:
 Any type of mixture can be separated by the Column
Chromatography.
 Wider choice of mobile phase.
 No limit of quantity as any amount of mixture can be separated by
this technique.
 It is a robust method.
 The separated analytes can be reused.
DISADVANTAGES:
 It is time-consuming process for the separation of compounds.
 It is expensive as higher quantities of solvents are required.
 The automated process becomes complicated and therefore costly.
 It has a low separation power.