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Conserved Sequence Motifs
Conserved Sequence Motifs
Conserved Sequence Motifs
SUBMITTED BY,
MITHILAA S
1ST MSc BIOCHEMISTRY
CONSERVED SEQUENCES
• The generalization and modification of already known motifs are becoming major
trends in the literature, even though new motifs are still being discovered at an
approximately linear rate.
• The emphasis of motif analysis appears to be shifting from metabolic enzymes, in which
motifs are associated with catalytic functions and thus often readily recognizable, to
structural and regulatory proteins, which contain more divergent motifs.
• The consideration of structural information increasingly
contributes to the identification of motifs and their sensitivity.
Genome sequencing provides the basis for a systematic analysis of
all motifs that are present in a particular organism.
• A systematically derived motif database is therefore feasible,
allowing the classification of the majority of the newly appearing
protein sequences into known families.
• Protein sequence motifs are signatures of protein families and can often be
used as tools for the prediction of protein function.
Abstract
An amino acid motif was identified that consists of the sequence
HisHydrHisHydrHydrHydr (Hydr--bulky hydrophobic residue) and is
conserved in two vast classes of proteins, one of which is involved in
initiation and termination of rolling circle DNA replication, or RCR
(Rep proteins), and the other in mobilization (conjugal transfer) of
plasmid DNA (Mob proteins). Based on analogies with
metalloenzymes, it is hypothesized that the two conserved His
residues in this motif may be involved in metal ion coordination
required for the activity of the Rep and Mob proteins. Rep proteins
contained two additional conserved motifs, one of which was located
upstream, and the other downstream from the 'two His' motif. The C-
terminal motif encompassed the Tyr residue(s) forming the covalent
link with nicked DNA.
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