Evaluating hyphal

spreading areas of Suillus

bovinus’ strains
On-site supervisor: Dang Hoang Quyen
IU Advisor: Assoc. Prof. Tran Thi My Hanh
Student name: Ton Ngoc Minh Trang
Student ID: BTBTIU18364
 Table of contents
Overview
01
The Applied Biotechnology Institute

Introduction
02
Ectomycorrhiza: Suillus bovinus background information

Conducted experiment
03
Measure and analyze spreading areas of 10 Suillus bovinus’ strains in 3 weeks

Discussion & Personal Judgement

04
Discuss the obtained data for the final conclusion
 01
Overview
The Applied Biotechnology Institute
 Overview

The Applied Biotechnology Institute:

❏ Established on June 22nd, 2021

❏ By Ho Chi Minh City Department of

Science and Technology

❏ Address: Fourth floor, Van Dat Tower, Lot

12F4, CN8 Street, Tay Thanh Ward, Tan

Phu District, Ho Chi Minh City.

 Overview
Organization structure:

a) Directorate board:
❏ Director: Ph.D. Pham Nguyen Duc
Hoang
❏ Vice Director: MSc Nguyen Tai Hoang

a) Departments:
❏ Mushroom Farming
❏ Physiology & Biochemistry
❏ Cryopreservation and Culture
❏ Taxonomy and Morphogenesis
❏ Molecular Biology and Genetics
 Overview

The corridor The morphological evaluation

room
 Overview

TheThe
storing samplesroom
inoculating room
 02
Introduction
Ectomycorrhiza: Suillus bovinus background information
 Mycorrhiza

● A mutual symbiotic association

between a fungus and a plant

● Play important roles in plant nutrition,

soil biology, and soil chemistry.
Ectomycorrhizal structure

+ Inside root: Intercellular hyphae

do not enter cells

+ Outside root:
● Mantle (Fungal sheath): Thick
hyphal layer around root
● Hartig net: Fungal hyphae
between cortical cells
● Hyphal mass ≈ root mass
 Suillus bovinus’ features

Origin
A common fungus native to
Europe and Asia

distribution
Common in Britain and Ireland
Pine forested areas throughout
mainland Europe
 Suillus bovinus’ features

Classification shape
A pored mushroom of the genus Initially convex cap
Suillus in the family Suillaceae Slimy and sticky

Color Dimension
Ranges from 3–10 cm (1¼–4 in) in
Whitish, yellowish or clay-
diameter
coloured
 Suillus bovinus’ features

Ectomycorrhizal root
Formations of the ectomycorrhizal roots
of a Pinus species with the fungal
species of Suillus bovinus

Fruiting body
Suillus bovinus’ edible fruiting
body occurs beneath pine trees
 03
Conducted experiment
Measure and analyze spreading areas of 10 Suillus bovinus’ strains in 3 weeks
 Procedure

- 10 strains from SB1-SB10

- Medium: Modified Melin-Norkrans
medium
- Duration: 3 weeks
- Time stone:
+ Week 1 (31st March - 7th April)
+ Week 2 (8th April - 15th April)
+ Week 3 (16th April - 23rd April)

- Criteria: Hyphal spreading areas

- Method: Hole-punching method
 First week results

SB1 - 07.04.22 SB2 - 07.04.22

SB3 - SB4 -
07.04.22 07.04.22
 First week results

SB5 - 07.04.22 SB6 - 07.04.22

SB7 - 07.04.22 SB8 - 07.04.22

 First week results

SB9 - 07.04.22 SB10 - 07.04.22

 Second week results

SB1 - 15.04.22 SB2 - 15.04.22

SB3 - 15.04.22 SB4 - 15.04.22

 Second week results

SB5 - 15.04.22 SB6 - 15.04.22

SB7 - 15.04.22 SB8 - 15.04.22

 Second week results

SB9 - 15.04.22 SB10 - 15.04.22

 Third week results

SB1 - 23.04.22 SB2 - 23.04.22

SB3 - 23.04.22 SB4 - 23.04.22

 Third week results

SB5 - 23.04.22 SB6 - 23.04.22

SB7 - 23.04.22 SB8 - 23.04.22

 Third week results

SB9 - 23.04.22 SB10 - 23.04.22

SPSS Analysis
 04
Discussion & Personal Judgement
Discuss the obtained data for the final conclusion
 Duncan Classification
STRAINS First week Second week Third week
SB1 0.59ab ± 0.26 1.64b ± 0.02 3.37cd ± 0.01

SB2 0.29d ± 0.02 0.61c ± 0.52 1.54e ± 1.41

SB3 0.28d ± 0.06 1.00c ± 0.11 2.22de ± 0.26

SB4 0.33cd ± 0.03 0.86c ± 0.11 2.33cde ± 0.13

SB5 0.46bcd ± 0.08 1.65b ± 0.16 3.32cd ± 0.15

SB6 0.54ab ± 0.04 1.98b ± 0.28 3.18cd ± 0.72

SB7 0.31cd ± 0.02 1.66b ± 0.14 3.50c ± 0.34

SB8 0.47bc ± 0.04 1.66b ± 0.14 4.88b ± 0.41

SB9 0.32cd ± 0.06 0.61c ± 0.33 1.64e ± 1.19

SB10 0.65a ± 0.06 3.15a ± 0.09 6.67a ± 0.34

 Discussion

● SB10 has the largest spreading areas and is

significantly different from the other.

● Provide more certain on finding the strains with the

highest hyphal spreading area

● Structural conformation of ectomycorrhizas with pine

roots under the laboratory conditions

 Personal judgement

Knowledge Soft skills

Ectomycorrhizal structures Teamwork & communication
and functions skills
Roles in ecology and Problem-solving and
environment organizing skills

Techniques
Micropipetting, culturing and
inoculating techniques
Using autoclave machine,
laminar air flow
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