POTENTIAL OF BIOLARVACIDES FROM Rhizophora Stylosa

MANGROVE LEAF EXTRACT IN GAMPONG PANDE AREA,

KUTARAJA DISTRICT, BANDA ACEH CITY

Dedy Syahputra, Sofyatuddin Karina, Irwan, Sri Agustina, Maria Ulfah

Marine Science Departemen, Faculty of Marine and Fisheries, Universitas

Syahkuala

August 03, 2021 3th ICFAES 2021 Banda Aceh

 Research Background
 Dangue fever  bioactive compounds
dad
 Spesies of mangroves

 Previous Research
1. Widhyastini and Hutagaol (2017) reported that secondary metabolites such flavonoids, tannins, and
saponins from natural product had the high biolarvacidal activity against mosquitoes larvae.
2. Some mangroves contained of secondary metabolites responsible as biolarvacidal agent against
Aedes sp. Are Rhizopora mucronata, Sonneratia alba, Avicennia marina, Xylocarpus granatum .

August 03, 2021 3th ICFAES 2021 Banda Aceh

 Objective of the research

the objective of the research was to

determine LC50 value of the R.
stylosa leaf extract.

91 90

Pa Th
Research Methodology

Time and Place

Gampong pande, Laboratory
Kutaraja dstict, of
Banda Aceh Parasitologi
From
December
2020 to march
Laboratory of 2021
Marine Chemistry
and Fisheries
Biotechnology
 methanol, aquadest,
Hg, KI, HCl, Chloroform, Rhizopora stylosa leaf
Materials anhydrous acetic acid,
buthanol, H2SO4, FeCl3
and Aedes sp. larvae,
evaporator
and Mg powder

Sample
extrction

Alkaloid Flavonoid (Mg Steroid dan Tanin

Phytochemic (Wagner powder and triterpenoid Saponin
(FeCl3
al screening (Liebermen (Aquadest)
reagent) HCl reagent ) reagent )
Burchad reagent)
 Biolarvacidal activity
Table 1. The relationship between
with the level of toxicity (Amelia and
Suyatno (2014)
The extract of R. stylosa was treated against Aedes
sp. by treating the larvae with 8 concentrations of
extract, namely; 0, 200, 400, 600, 800, 1000, 1200
and 1400 ppm with 2 repetitions

Category (mg/L)

Tidak Beracun > 10000 The extract solutions were dilluted with aquadest and
Biolarvacidal stirred using magnetic stirrer. The bioassay was 10
Rendah activity
1000 – 10000 individuals of Aedes sp. larvae (instar III). The larvae were
treated in 50 ml extract solution using beaker glass as
Sedang 100 – 1000 container within 48 h of observation.

Beracun 1 – 100

Sangat Beracun <1 The mortality value then was measured as follow the formula P
= and The value of LC50 was determined using probit analysis
method statistically (Eriani et al., 2020). while the toxicity level
was categorized according to Table 1.
 Result and Discussion
 Phytochemical screening
• in its bark except alkaloids (Amelia and Suyatno,
2014)
No Group compound Test results
• in leaf, fruit and root of Avicennia marina except
alkaloids, fenolics and glycosides (Eriani et al.,
_
1 Alkaloid 2020).
• Those biolarvacidal agents; flavonoids, tannins,
2 Flavonoid +
saponins and triterpenoids play the important role
3
Saponin + in larvae mortality. According to Siamtuti et al.
(2017 and Kumara et al. (2021),
+
4 Triterpenoid
Steroid _
5
Tanin +
6
 Mortality of Aedes sp.
100

90

80

70

60
% Mortality

50 P=
40

30

20

10

0
0 200 400 600 800 1000 1200 1400 1600
Concentration (ppm)
Based on probit analysis, the LC50 value of R. stylosa leaf extract was found 858.89 ppm.

extract of bark of Rhizophora

mucronata (78,819.5 ppm) and all (Andriyani et al.,
part of Sonneratia alba (>1000 2018; Usman et al.,
ppm), where as categorized as non 2020).
toxic and low toxic

the LC50 value of R. stylosa leaf

This research
extract was found 858.89 ppm.

root of Rhizophora apiculata (6.85 ppm) (Sari, 2017;

and from leaf (2.72 ppm), fruit (1.58 ppm) Eriani et
and root (1.23 ppm) of Avicennia marina,
where as categorized as toxic al., 2020).
 Conclusion
Based on the study, it was
obtained that R. stylosa
leaf extract contained of
flavonoids, saponins,
triterpenoids and tannins.
The optimum
concentration of the
extract was 1,200 ppm,
while its LC50 value was
858.877 ppm, so it was
concluded that the the
extract toxicity against
Aedes sp. was moderate.