BC-6000 service training

air dynamic and liquid system

IVD Global Technical Support Dept.

Version ： V2.0
Number: MXQ-18027-BC-6000

© 2018 Mindray Confidential

 Improvement and advantage
 For better understanding:

BC-6200 = Similar BC-5300 sampling & HGB & RBC

+ Optimized BC-6800 optics
+ New air system.

 Shut down procedure is associated with “auto-protect”

• If “auto-protect” or “whole instrument maintenance” has
been done in the same day, shut down procedure could finish
in seconds.
 Fast start up initialization. Only 3~4 minutes.
 Fast aspiration, only once piercing for one test tube.

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 Components – Valves

LVMK valve for air

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 Components – Valves

Two Way Valve

① ②
① is inlet
② is outlet

Three Way
Valve
③ ① ②

① is NC
② is COM
③ is NO)

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 Components – Valves

LVM valve for accurate sample aspiration

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 Components – Valves

LVM valve for accurate sample aspiration

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 Components – Valves

Burcket valve for high flow

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 Components – Valves

Mindray valve for common purpose

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 Components – Valves

High pressure Mindray valve for

strong pressure.

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 Components – Check Valve

Check valve to make sure

liquid only go in one way. In
order to prevent reagent mix in
the tubing.

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 Components – Check Valve

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 Components - Air Pump

 Bomba nueva, más grande que la bomba de desecho.

 Use SV63 para expulsar el agua del aire comprimido.

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 Components - Waste Pump

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 Components - Waste Pump Filter

 Protect the waste pump.

 Flush to clean, don’t open them.

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 Components - N40KPa Chamber

Bottom

Floater to prevent over flow.

Manually drain out by tube T318

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 Components - 120KPa Chamber

No floater, use SV63 to drain out

accumulated humidity during start
up and sleep mode.

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 Components – 90KPa and 50KPa Chamber

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 Components – 4 Chambers

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 Components – Vacuum/Waste Chamber

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 Pressure and Vacuum

No air compressor, only one air pump

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 Pressure and Vacuum

Release vacuum to
get accurate -30KPa

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 Pressure and Vacuum

940
920
900
880
90kPa 860
840
820
800
200 250 300 350 400 450 500

315

310

305

N30kPa 300

295

290
200 250 300 350 400 450 500

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 Service Tips

 How to release pressure and vacuum.

 If login as service, there is a button in the pressure/vacuum status screen to
release and establish the pressure.
 When powered off, disconnect the tubing on the 5 pressure sensors to
release.
 How to stop liquid flow.
 To cut the diluent flow, please bend the tubing below DIL and FCM
chamber.
 Before disconnecting the liquid chamber for lyse, please drain out first.

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 Components – Probe and Wipe Block

 New probe, no air hole, no cut on the side wall.

 Prevent blood stain.
 Wipe block position is fixed.
 Probe holding position is fixed.
 No need for realignment on change.
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 Components – Probe and Wipe Block

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 Components – Probe and Wipe Block

 Sample aspiration and dispensing

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 Components – Probe and Wipe Block

 Sample aspiration and dispensing

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 Components – Syringes

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 Components – Reagent Chambers

DR LD LN LH

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 Components – Check Valve

Check valve to make sure

liquid only go in one way. In
order to prevent reagent mix in
the tubing.

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 Components – Chambers

Air pump 1

VC -30KPa FCM DIL

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 Components – WC1 & WC2

WC2

WC1

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 Components – RBC bath

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 Components – HGB bath

New design, diluent and LH lyse goes in from the bottom side
to make circle flow for mixing. Needs less bubble for mixing.

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 Components – Reagent Preheat Chamber

Warm up sheath fluid, HGB diluent, RBC diluent and diluent cleaning the
sample preparation tube. Target is 32 ℃
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 Components – WNB and DIFF reaction bath
assembly

 Integrated with heating system.

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 Components – RET reaction bath
assembly

Not functional

RET

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 Components – DP

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 Components – Pinch Valve

 Smaller size, lower pressure.

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 Reagent
M-6 Function
DS Dilute sample Sheath Flow
Diluent
M-6DR Spherify the cells
M-6LD Break down RBC in Diff channel
M-6LN Break down RBC in NRBC channel
Lyse M-6LH Break down RBC in LH channel
Break down RBC in Baso channel; Shrink
/
all white blood cells except for basophil
M-6FD Fluorescence dye in Diff channel
Dye M-6FN Fluorescence dye in NRBC channel
M-6FR Fluorescence dye in Ret channel

M-6 reagents could be use on BC-6000/BC-6200 and BC-6800 Plus

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 Reagent Consumption

M-60 M-60 M-60 M-60 M-60 M-60 M-60 DS

LD FD LN FN DR FR LH

1.56 1.56 1.56 0.52 36-45

20ul 20ul 20ul
mL mL mL mL
mL

DIFF WNB RET HGB RBC

0.52 ml LH lyse will be used for cleaning purpose. So, DS and LH

consumption maybe different for different test modes.

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 Sequence – Sample Aspiration
Normal openVC
valve

ON

ON

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 Sequence – Sample Aspiration

OFF
ON

OFF

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 Sequence – Sample Aspiration
Atmosphere

Improvement: ON
OFF
 No bubble in sample aspiration tube.
 No blood stain on test tube capOFF
 100,000 times of piercing ON

No movement

Atmosphere

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 Sequence – Sample Dispensing

W
HG

DI
RE

NB

FF
B
T

RB
C

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 Chamber Priming

Primed by syringe

Big DP8 like an extra

DIL chamber

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 Sample Reaction

Controls the speed of LN

Controls DP3 and DP4
dispensing

Controls DP1 and DP2

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 Sample Preparation

 Exclusive path for RET sample:

 FR may affect IMG in Diff
 FN may affect RET*

 Troubleshooting:
 The sample flow is visible if put a white paper behind the joint
below flow cell.

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 Establish Sheath Flow

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 Auxiliary Sample Injection and Optical
Counting

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 Clean

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 RBC Counting
Aspirate the 1st dilution
sample from HGB bath

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 RBC Cleaning

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 HGB Detection

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 Waste Collection

 WC1: Keep N40kPa. Drain out all the

reaction bathes. N30 also comes from
WC1.
 WC2: Open to the atmosphere from the
left bottom of analyzer.
 P2: Exclusive for wipe block
 P3: Exclusive for WC1 draining

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 Wearing Parts
Name Part Number Description When to Change
100,000 times of piercing (software
PN 115-033912-00 Piercing Needle
reminder)

PWD 041-023727-00 Probe Wipe Annually or together with probe.

LF1 115-011660-00 Liquid Filter for Probe Wipe Annually

LF2 115-011660-00 Liquid Filter for Waste Annually

LF3 0010-10-12408 Liquid Filter for Diluent Annually

LF4 115-010567-00 Liquid Filter for Sheath Flow Annually

Pinch Tube M6G-020011--- Yellow Pinch Tube Annually

Pinch Tube for

082-000614-00 Thick Pharmed Tube Annually
Sample Preparation

P2 3101-30-68351 Waste Pump for Wipe Block 3,000H (Software Reminder)

P3 3101-30-68351 Waste Pump for WC1 3,000H (Software Reminder)

P1 082-002562-00 Air Pump 3,000H (Software Reminder)

56 © 2018 Mindray Confidential

 Version Information

Version Editor Date Description

Number

1.0 Marcus 2018-6-28 First release

2.0 Poly 2020-05-06 Modify the error

3.0 POLY 2020-07-14 Replace the wrong map in P52

57 © 2018 Mindray Confidential

 Review Information

Version Reviewed Review Date Revision Details

Number by

1.0 Luke 2018-6-30 First release

2.0 Luke 2020-05-08 Modify the error

3.0 Luke 2020-07-14 Replace the wrong map in P52

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 Thank you!

59 © 2018 Mindray Confidential