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FOTOSINTESIS

Energi sinar matahari


Reduction Reaction
• The gain of electrons to a substance.
• Or the loss of oxygen.
oxygen

Reduction

6CO2 + 6H2O  C6H12O6 + 6O2


glucose
Breakdown of Photosynthesis
• Two main parts (reactions).

1. Light Reaction or
Light Dependent Reaction

Produces energy from solar power (photons) in the


form of ATP and NADPH.
NADPH
Breakdown of Photosynthesis
2. Calvin Cycle or
Light Independent Reaction or
Carbon Fixation or
C3 Fixation

Uses energy (ATP and NADPH) from light rxn to


make sugar (glucose).
Photosynthesis Dark Reaction

H 2O CO2
Light Reaction

Energy
H+
ATP and
Which splits NADPH2

water

Light is Adsorbed ADP + P ATP NADP + H NADPH


By
Chlorophyll

Chloroplast

Calvin Cycle
O2
Dark Reaction C6H12O6
Light Reaction
1. Light Reaction (Electron Flow)
• Occurs in the Thylakoid membranes

• During the light reaction,


reaction there are two possible
routes for electron flow.
flow

A. Cyclic Electron Flow


B. Noncyclic Electron Flow
Diagram Reaksi Terang
A. Cyclic Electron Flow

• Occurs in the thylakoid membrane.


membrane
• Uses Photosystem I only
• P700 reaction center- chlorophyll a
• Uses Electron Transport Chain (ETC)
• Generates ATP only

ADP + P ATP
A. Cyclic Electron Flow

Primary e-
SUN Electron
Acceptor

e- e- ATP
produced
Photons by ETC
e-
P700
Accessory
Pigments
Photosystem I
B. Noncyclic Electron Flow
• Occurs in the thylakoid membrane

• Uses PS II and PS I

• P680 rxn center (PSII) - chlorophyll a

• P700 rxn center (PS I) - chlorophyll a

• Uses Electron Transport Chain (ETC)

• Generates O2, ATP and NADPH


B. Noncyclic Electron Flow
Primary
Electron 2e-
Acceptor
Enzyme
Primary Reaction
2e-
Electron
Acceptor
2e-
ETC

SUN 2e-
2e- NADPH
P700
Photon ATP
P680 Photon
H2O Photosystem I

1/2O2 + 2H+ Photosystem II


B. Noncyclic Electron Flow
• ADP + P  ATP
(Reduced)

• NADP+ + H  NADPH
(Reduced)

• Oxygen comes from the splitting of H2O, not


CO2

H2O  1/2 O2 + 2H+


(Oxidized)
Chemiosmosis
• Powers ATP synthesis.
synthesis

• Located in the thylakoid membranes.


membranes

• Uses ETC and ATP synthase (enzyme) to make ATP.

• Photophosphorylation: addition of phosphate to


ADP to make ATP.
ATP
Chemiosmosis
SUN (Proton Pumping)
H+ H +

Thylakoid E
PS II T PS I
C

high H+
H H
+ +
concentration
H+ H + H + H+

Thylakoid
H+ ATP Synthase Space

ADP + P low H+
ATP
H+ concentration
Daur Reaksi Gelap (Daur Calvin)
Diagram Reaksi Terang dan Gelap
dari Fotosintesis
Calvin Cycle
• Remember: C3 = Calvin Cycle

C3

Glucose
Photorespiration
• Occurs on hot, dry, bright days.
days

• Stomates close.

• Fixation of O2 instead of CO2.

• Produces 2-C molecules instead of 3-C sugar


molecules.
molecules

• Produces no sugar molecules or no ATP.


Photorespiration
• Because of photorespiration:
photorespiration Plants have special
adaptations to limit the effect of photorespiration.
photorespiration

1. C4 plants
2. CAM plants
Daur C4 (Hatch-Slack)

bentuk senyawa HCO3


Daur C4 (Hatch-Slack)
C4 Plants
Malate Malate
C-C-C-C C-C-C-C
Transported CO2
CO2 C3

Vascular
Tissue
glucose
C-C-C
PEP ATP
C-C-C
Pyruvic Acid

Mesophyll Cell Bundle Sheath Cell


CAM Plants
• Hot, dry environments.
environments
• 5% of plants (cactus and ice plants).
• Stomates closed during day.
• Stomates open during the night.
night
• Light rxn - occurs during the day.

• Calvin Cycle - occurs when CO2 is present.


CAM (Crassulacean
( Acid Metabolism) Plants

Night (Stomates Open) Day (Stomates Closed)

Vacuole

C-C-C-C C-C-C-C C-C-C-C


CO2 Malate Malate
Malate

CO2
C3

C-C-C
ATP C-C-C glucose
PEP
Pyruvic acid
Question:
• Why would CAM plants close their stomates during
the day?

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