BC5000¡ BC5150 Service Training Material1.0

BC-5000/BC5150 service training
© 2013 Mindray Confidential

Content
 Clinical
 Overview
 Principal
 Installation
 Software
 Hardware
 Optical system
 Liquid system
 Mechanical structure
2 © 2013 Mindray Confidential

clinical—Blood Cells
 Blood consists of 40-50% blood cell and 50-60% plasma.

 Three main parts of blood cell:
red blood cell, white blood cell and platelet.

Five groups of leukocyte
WBC 5-part proportion
 Lymphocyte 25 ~ 30%
 Monocyte 3 ～8%
 Neutrophil 50 ~70%
 Basophil 0 ～1%
 Eosinophil 0.5 ～ 3%

 Morphology ： size, shape, cytomembrane, cytoplasma, nucleus,

granule.
RBC WBC PLT

GRAN MONO LYMPH
NEU ESO BASO

Clinical—Blood Cells

Morphological

Clinical—WBC five part comparison
NEU EOS BASO LYMPH MONO

Diameter 10~14um 11~16um 10~12um 6~9um 12~20um
Cytoplasm Large more more less More than

amount LYM
karyon Multlobed, 2lobes mostly 3~4lobes, or Large round Irregular and
2~5 hard to indented
divide
Granule Neutral Eosinophilic basophilic None normally azurophilic
or
acidophili
c
Chromatin More lump More lump More lump Tightness loosen
evenly
Color of Reddish Reddish Reddish Baby blue Grey blue or
cytoplas little
m reddish

Clinical —granules in cytoplasm
Neutral Eosinophilic basophilic Azurophilic
Size Fine and even Thick and even Most thick and Thicker than
uneven NEU, uneven
Shape Fine particles Round or ellipse Differ in shape Differ in shape
Amount Much Much Less A few or

moderate
Color Pale violet red Orange red or dark Royal purple or Purple red
or reddish yellow dark purple
Distribution Even Even Uneven, coat the Uneven, coat the

nucleus normally nucleus
occasionally

Content
 Clinics
 Overview
 Principle
 Installation
 Software
 Hardware
 Optical system
 Liquid system

Overview—appearance
 BC-5150 BC-5000

Overview —Dimension
BC-5000
vs BC-2800
324mm 322mm
437mm
415mm
410mm 386mm

Overview —Report parameter
White Blood Cell count WBC Red Blood Cell count RBC
Basophils number Bas# Hemoglobin Concentration HGB
Basophils percentage Bas% Mean Corpuscular Volume MCV
Neutrophils number Neu# Mean Corpuscular Hemoglobin MCH
Neutrophils percentage Neu%
WBC Eosinophils number Eos# Mean Corpuscular Hemoglobin Concentration MCHC

11 RBC
Para Eosinophils percentage Eos% 8
Para Red Blood Cell Distribution Width Coefficient RDW-CV
Lymphocytes number Lym# of Variation
Lymphocytes percentage Lym% Red Blood Cell Distribution Width Standard RDW-SD
Deviation
Monocytes number Mon#
Monocytes percentage Mon% Hematocrit HCT
Platelet count PLT
Mean Platelet Volume MPV
PLT
Platelet Distribution Width PDW BC5150 only
6 Plateletcrit PCT
Para
Platelet-large cell ratio P-LCR
Platelet-large cell count P-LCC

Overview —RUO
BC5150 only
Name Abbr.
Abnormal lymphocytes number ALY#
Abnormal lymphocytes Percentage ALY%
Large immature cells number LIC#
Large immature cells percentage LIC%

Overview —appearance
1 、 Display screen
2 、 Power/status indicator
3 、 Probe wipe block
4 、 Sample probe
5 、 [ Aspirate] key
 Front of the analyzer

Overview —appearance
1 、 M-52D diluent inlet

2 、 Waste outlet
3 、 Waste sensor
4 、 AC input socket
5 、 Power switch
6 、 Air outlet for fan
7 、 Back panel
 Back of the analyzer

Overview —Main screen

Overview —difference between BC5000andBC5150
item BC-5000 BC-5150

Test speed 40Samples/h 60Samples/h
Report Para 23 25
RUO NONE 4Para
Sample storage 20000 份 40000 份
WBC linear 0.00×109/L ～ 100.00×109/L 0.00×109/L ～ 500.00×109/L
PLT linear 0×109/L ～ 1000×109/L 0×109/L ~ 5000×109/L
Front panel 043-002705-00 043-002706-00
Screen assembly 115-018417-00 115-018418-00
Key board 043-002703-00 801-3100-00194-00

Overview —Specification
 Measuring mode ： CBC 、 CBC+DIFF

 Sample mode ： WB Capillary W PD
 Sampling ：
 WB Capillary W
 Sample volume of aspiration ： CBC+DIFF—15ul
CBC—11.5ul
 OV-PD
 Sample volume of aspiration ： 200ul
 Diluent dispensed ： 480ul
 Blood collected ： 20ul
 Scattergram ： DIFF(Report:L-M) ，（ RUO ： L-W 、 M-W)
 Histogram ： WBC 、 RBC 、 PLT

Overview —Specification
 Operating temperature ： 10℃—30℃

 Screen ： 10.4’LED ， with touch screen
 Weight ：≤ 25Kg
 Interface ： 4USB, 1 ethemet network port
 Data transmission ： HL7 supported
 Printer ： external ， PCL6
 printer ： EPSON LQ-590K 、 HP Laser Jet P1505n 、 HP OfficeJet Pro
K5300 、 HP LaserJet P1606dn

Overview —reagent
Reagent Name Type
Diluent M-52D DILUENT M-52D
M-52DIFF LYSE M-52DIFF

Lyse
M-52LH LYSE M-52LH
Probe cleanser PROBE CLEANSER /
Control : Mindray made

Calibrator : R&D: S-CAL Mindray made ： S50

目录
 Clinics
 Overview
 Principle
 Installation
 Software
 Hardware
 Optical system
 Liquid system

Principle —main technology
colorimetry
Laser Lambert
scattering –Beer
law
Impedance
Lyse
Flow
without
cytometry
cyanide
Cytochemical
staining

Principle—system block diagram

Principle—Flow cytometry
 Flow cytometry + Laser scattering—WBC 5DIFF
 Sheath fluid surrounds and forces blood cells suspended in dilution
to pass through the center of flow cell one by one.
Blood cells pass through the facula of flow cell with high speed
cell
Laser facula
Flow cell
sheath
sample

Principle—twice accelerating sheath flow
Flow cell Scatter light

Laser
sample
2ed Sheath flow 1st sheath flow
Blood sample

Principle—Multi-angle laser scattering
 Multi-angle laser scattering ；

 LAS—volume of the cells ；
 MAS—inner complexity
 WAS—granularity of the cells

Principle—cytochemical staining
 M-52 DIFF Lyse ：
 Cationic surfactant To dissolve the RBC ， special treatment to
WBC
 anionic groupMono
Lymph stain the
Nuegranular
Eos of the
BasoWBC

original
After adding
reagent
More difference in complexity

Principle—cytochemical staining
 M52-LH Lyse
 To beak down the RBC
 All the cells are naked (no membrane) except Baso, the Basophils are relatively
stable in size and inner structure, larger in volume than other kind of cells,
 To converts hemoglobin to a hemoglobin complex
original
After adding
reagent
Lymph Mono Nue Eos Baso

Principle—scattergram
Neu
Mon
Lym
Baso
Eos

Principle—L-M Scattergram （ report ）
 DIFF ： laser scattering+cytochemical staining
Monocyte
Neutrophil
Lymphocyte
Eosinophil

Principle—scattergram (RUO)
 L-W scattergram ， M-W scattergram ， special for Eos ， more

accurate to large amount of EOS.
Eosinophil

Principle—histogram of Baso
 laser scattering + cytochemical staining scattergram

histogram
Basophil

Principle—position of cells
Scattergram
DIFF
Histogram
LAS
LIC
LIC
MON NEU
ALY EOS
LYM
Nrbc
BAS
GHO
MAS

Principle—HGB
 HGB measurement
 Lambert-beer law
 HGB(g/L) = Constant×Log 10 (Blank Photocurrent/Sample Photocurrent)
Fe 2+ 3+
Fe
＋ Fe 2+ Fe
3+
Fe 2+ Fe
3+
Fe 2+ Fe
3+
Anionic surfactant
Lyse without cyanide

Special surfactant
ferrous iron is oxidized to ferric iron
Principle—RBC/PLT
 Impedance method

Principle—RBC/PLT
Time metering
 principle
 Volume metering ： Use fixed volume of
metering tube to fix the quantity of each
test sample, Triggered by the optical
sensor 。
 Time metering ： fixed test time to fix the
quantity of each test sample
volume=test time X velocity of the flow
Use large vacuum chamber(200ml)

to ensure stable negative pressure

Principle—RBC/PLT
 Test time
• Accurate hardware timing ： RBC 9s
 How to ensure stable flow velocity

• Stable negative pressure
• Abnormal monitoring
1.Stable aperture voltage

2.Stable particle stream
Aperture voltage: 14.75-

23.75

Content
 Clinics
 Overview
 Principle
 Installation
 Software
 Hardware
 Optical system
 Liquid system

Installation—procedure
 Preparation
 Installation ： check up, unpacking, emplacement, connection,
 Starting up and login
 Setting
 performance verification
 Background, reproducibility
 Quality control and calibration(if needed)
 Scattergram confirmation （ blood sample ）
 Other setting ： customized setting, maintenance, print.
 Customer training

Installation—preparation
 Tools ：
 Blade or clipper
 USB flash disk
 Pipette (200ul)
 Cross-headed screwdriver
 Control (normal)
 Calibrator

 Environment ：
 should be as free as possible from dust, mechanical vibrations, and
electrical interference;
 Far from brush-type motors, flickering fluorescent lights, and electrical
contacts that regularly open and close;
 Do not place the analyzer on a slope;
 Do not place the analyzer in direct sunlight or in front of a source of heat
or drafts;
 The environment shall be well ventilated

 Space ： shown as in figure

enough room on or below the countertop to accommodate the
diluent and waste containers.
 Voltage ： 100V-240V ～， 50Hz/60Hz
 Input power ： ≤ 300VA
20cm
 Standard configuration ：
 Analyzer, reagents
 Optional ： printer , barcode scanner
50cm

Installation-unpacking
 Check ：
 outer packing and apparatus
 Packing list and contents
 Apparatus arrangement

Installation—connection
Power switch
M-52D
diluent inlet
Waste outlet Waste sensor connector

Power socket


M-52DIFF lyse connector

M-52LH lyse connector

 Peripheral devices ：
Printer
Barcode scanner
 Computer
Network cable
Data management software
USB port and network port

Installation—Starting up
Hardware self test, liquid system self test and maintenance, background
test 。
User ID ： service
Password ： Se s700 （ capital S, blank between Se and s700)


Installation—Setting up
 reagent
press
press

 reagent
press
Scan
or
input

 Other settings

Installation—performance test
 Background Verification
Parameter Background/ blank count

requirements
WBC ≤ 0.20 109 / L
RBC ≤ 0.02 1012/ L
HGB ≤1 g/L
HCT ≤0.5 %
PLT ≤ 10  109 / L



 Reproducibility

 Reproducibility

Installation—quality control
press
Installation—quality control
 Click “By SD” to display the limits in the form of absolute value;
 Or click “By CV” to display the limits in the form of percentage
Content
 Clinics
 Overview
 Principle
 Installation
 Software
 Hardware
 Optical system
 Liquid system

Content
 Clinics
 Overview
 Principle
 Installation
 Software
 Hardware
 Optical system
 Liquid system

Software—calibration
old factor  reference value

new factor 
calculated mean
Transfer factor =CBC CBC+DIFF
Analysis Result = measurement result x factory’s factor x user’s factor x transfer

factor

Factory
calibration
factor and
tansfer factor
with the engineer permission, factory’s Cal factor and transfer factor will
be modified, meanwhile the user’s factor will be adjusted to 100%

User’s factor
only
with the administrator permission, user’s Cal factor is modified, it’s

recommend to be with “Admin” permission to login and calibrate

Software—gain
Target :4.5V
HGB gain can be modified manually, to modified the [set value] to

change the HGB blank voltage to around 4.5

Software—advanced toolbox
 Change language
 Choose the type of data saved
 Update
Software—one key export
U disk ：
Kingston 8/16G
SanDisk 8/16G
Maxell 8/16G
FAT32
to reserve 4G
space.
Analyzer Info, software adjust Info, closed-reagent Info. Save to USB flash
disk, files named10000 和 10000_service, if the SN of the analyzer is 10000

Software—update
 Create an upgrade USB drive

Unzip update.rar and copy the unzipped “update” directory to the root directory
of the USB drive.
 Upgrade
 Insert the USB drive into the USB port on the analyzer. Enter the Advanced
Toolbox and press[update] to upgrade the software
 The upgrade process can be divided into two steps.
• Step 1: upgrade the guidance and operation system
• Step 2: upgrade the software components
When upgrading the boot and operation system, the operator will be prompted
to restart the analyzer between step 1 and step 2.
When upgrading the software components, the upgrade process will directly go
to step 2.

Software—communication
IP addresses of the PC and the analyzer shall be in the same

IP segment
Default IP of the analyzer ： 10.0.0.2
Software—communication （ Mindray Hematology data
management Communication soft ware)
Port :5100
Analyzer’s IP
 Select the actual analyzer model

 the encoding mode of HL7 communication protocol is UTF-8,
and that of 15ID protocol is GBK

Software—communication （ LIS ）
IP can
be
changed
contact your network administrator as to setting up IP address 、 subnet

mask and default gateway
Content
 Clinics
 Overview
 Principle
 Installation
 Software
 Hardware
 Optical system
 Liquid system

Hardware—boards
 Main board(digital control board)

 Analog drive board
 Power supply board
 Indicator board
 Touch screen control board
 Laser control board
 Pre-amplifier board
 MAS&LAS PD board
 WAS PD board
 Liquid sensor board

Hardware—system overview
Front panel Optical system

Touch
screen Laser PD
control
LED Screen
board
Touch screen Pre-
Laser drive
Indicator amplified
board
board board
Aurepert
Digital main board Analog driving board
HGB unit
Valve,
USB Motor floater
Power pump
supply
board Temp
Net Sensor hydraulic
control
Fluidic

Hardware—system control
Data Channel Master Control

Sample review Touch screen
Signal Signal
Sensor
preprocess sampling Result display TFT monitor
Signal
preprocess Result print USB printer
Channel Control Data storage
Data manager Network

interface
Bar-code
Input module
scanner
Drive/Detect
Control Start key
Motor Optical Alarm

Valve and
switch
pump control control control control
POWER Supervisor
POWER supervise system Indicator
board
State
supervise

Hardware—connection
Pinaster Master Analog&driver
Control Board Board
J1 Float
Ethernet J1
Communication J5 HGB
J81 J31
USB J2 J6 RBC
Optical
Analog Data J9 system
USB J3 J85 J4
Temperature
J10 sensor
BackLight J4 J11 Liq pressure
Supervise Signal sensor
LCD J86 J2 Optical
Data J16 J12 switch
Optical
Control Signal
J13 switch
Touch Screen J67 J77 J3
J14
Valve
Indicator J78 J15
J16 Pump
Start Key J99
J68 J17 Fan
P5V P12V J18 Fan

POWER Board
J19 Fan
D5V J1 Waste
J20
Pump
P12V/P24V J2 P5V
P12V J22 J21 Heat
A+12V/A-12V J3 P24V
J23 Motor
A+12V/A-12V
AC120V J4 J8 J24 Motor
AC120V

Hardware—Pinster main control board
 Composition ： Digital section, analog section

 Function:
 A/D conversion
Converts the analog signals to digital signals which can be processed by FPGA or
CPU.
 Data processing
The digital signals obtained from A/D samples are digitally filtered and save by
FPGA, then is transmitted to CPU for further processing, output the result.
 Peripheral interface implementation
The CPU module provides a platform for system software, interfaces for peripherals
 Control interface extension
Provides control logic and interface for LCD, SD card and touchscreen, etc

Hardware—Pinster main control board

Hardware—analog drive board
 composition ：
 Power module
 Control module
 monitoring module
 Power drive module

 Power module
supplies power for all the electronic devices on the analog drive board, including both
input power from the analog drive board, and the power converted by the chip;
 Main control module
responsible for communicating with the control board and task scheduling;
 Monitoring module
collecting information such as temperature, pressure, voltage and fluid in the tubes;
 Power driving module
driving electric components such as motor, heater, fan, valves and pumps;
 RBC detection circuit
provides the RBC module with constant current source, RBC signal collection and
modulation;
 Modulation of optical signals and HGB signals.


Hardware—Power supply board
Name Socket No. Description
PIN1 ： GND
D5V J1
PIN2 、 PIN3 ： 5V
PIN1 ： GND
P12V PIN2 ： 12V
J2
P24V
PIN3 ： 24V
PIN1 、 PIN4 ： GND
A+12V A- PIN2 、 PIN3 、 PIN5 ： -

J3
12V 12V
PIN6 ： 12V
AC120V J4 Alternating current

Hardware—Indicator board
 Indicate the current status of the analyzer by means of sound and

light

Hardware—status of indicator
Analyzer Status Indicator Comments
Ready Green light on Sequence is allowed
Running Green light flashing Sequence is being performed
Running with error Red light flashing An error is present and the
system is running
Stop with fault Red light on An error occurs and the
system is not running
No fault is present, but Yellow light on Initialization and standby
fluidics action is not status of sequence is not
allowed involved in the startup process
Enter/Exit standby status Yellow light flashing Enter/Exit standby status

Hardware—buzzer
Event Buzzer Prompts Comments
Startup completed One short beep Startup completed means the whole
startup process has been completed
and the analyzer is ready for operation
Open-vial aspiration completed Two short beeps
When count operation can not be One long beep If these screens have already
started in count related screens (including prompted, then the buzzer need not be
Sample Analysis, QC, Calibration, responded again.
Reproducibility, Carryover, Background,
Aging and Optical Gain Calibration
Count, etc.), press the aspirate key
Error Long intermittent Tap the touchscreen to stop the
beeps buzzer
Analyzer is ready One short beep Analyzer enters ready status from
other status
When the screen is black prompting stop beep If an error occurs during the
“Please power off the analyzer” shutdown process, the buzzer will
stop beeping when the screen is black

Hardware—touch screen drive board
 provides the interface between the touchscreen and the

control board
 converting the user's touch operation to identifiable signals
for main board.
 The touch screen drive board needs to be adjusted before
use.

Hardware—Liquid sensor board
 LED and Test point TP4

 output lower than 0.8V, the LED light is off, (have reagent)
 output higher than 2V, the LED light is on, (no reagent)

Hardware—optical boards

 MAS&LAS PD board
 WAS PD board

Hardware—Laser control board
 to provide stable and moderate-intensity driving current for laser

 Power modulation
 Laser driving current monitoring
 Laser power control.
 VR1:to adjust the power of the laser
Variable
Resistor
Control Laser driver Laser

(Closed-loop control) （HL6714G）
Voltage Laser current Power

supervise supervise PreProcess
A±12V
Laser Control
Board

Hardwar—Pre-amplifier board
 Power modulation
 I/V conversion
 Signal modulation.

Content
 Clinics
 Overview
 Principle
 Installation
 Software
 Hardware
 Optical system
 Liquid system

Optical system—introduction
 Simple structure, easy to maintain

 Omit backward optical assembly, to receive scatter signal directly with a
large size PD instead, make it small and low cost
3106 optical
3107optical

 Add a new signal sensor to collect the scatter at a wide angle, and the path
called WAS, three angles to collect scatter signal to for three-dimensional
scattergram that ensure the accurate category to the special sample ，
MAS
Collector Lens2
MAS Aperture
Collector Lens1
LD
Flow Cell Beam Splitter
Collimating Lens Cross Cylindrical
Lens
LAS Aperture
LAS
Flowcell Assembly
Light Source Assembly
Scattering Detection Assembly

Optical system—principle
 To form an elliptic facula
Front view Focus point
Lasrer Collimating lens Cylindrical lens Light source assembly Flow cell
Focus point
Top view

Facula and signal ： light intensity in horizontal direction
V
99 © 2013 Mindray Confidential 样本流

 Oval facula -Gaussian distribution

 Vertical direction: cover an entire blood cell
 Horizontal direction ： wider than that of sample flow, even light
intensity in detection area.
cell
Laser facula
Flow cell
sheath
sample

 To collect the scatter light directly with PD assembly

Optical system—structure
 1-Light source assembly 5 4

6
 2 -Flow cell assembly 7
 3- base plate assembly
 4-Preamplifier and shield
shell
 5-Shield assembly
 6-Scattering detection
assembly
 7-Laser control board
3
2
1

Optical system—structure

Optical system—boards

Variable
Resistor

Optical system—temperature control
Item Temperature °C
Interior temperature 15~40 （ alarm ）
Fan <25 ， >30
Optical System
Temperature Testing Unit
J10
J10
J17
J17
Analog
Analog Drive
Drive J18
J18 Fan System
Board
Board J19
J19
 Heating ： power supply board

 Heat dissipation ： fan

Optical system—status confirmation
 To confirm the performance of optical system,

 CG position-intensity of the signal
 CV-signal stability
 Enter the “optical” screen, perform counting with 7um standard
particle solution (0.5ml deionized water+3drops standard particle ）
Parameter Total CG Position CV

LAS 38~42 ≤6.50
MAS 1500~3000 107~158 ≤3.00
WAS 135~220 ≤8.00


Optical system—maintenance
 Auto maintenance
 Clean the flow cell
Enter “maintenance]”–[clean] screen, press [flow cell],
 Probe cleanser soak
 Enter “maintenance]”–“maintenance]”–[overall]
 Perform the maintenance manually

 Normal facular

 Abnormal facula
 Use dust-free cloth with dehydrated alcohol to wipe the outer surface of
cylindrical lens ；
 Replace laser control board ；
 Replace the optical system ；
Abnormal
facula

 If the exterior or interior of the flow cell is very bright, then the
exterior or interior may be stained.
 Use dust-free cloth with dehydrated alcohol to wipe the outer
surface of the floe cell.
 Auto or manual Before Cleaning After Cleaning
Clean the inner of the flow cell

 Verify that the light beam is perpendicular to the flow cell

 Place an inner hexagon spanner in the path of the light. The output spot
and the spot reflected by the flow cell should overlap and strictly
coincide with each other
Output Spot
Reflection Spot

Optical system—replacement
 Shut down, disconnect the cables and fluidic tubes carefully.

 Unscrew
 Replace
 Drain out the residual fluid with syringe

Optical system—scattergram
Center of gravity is lower Scatter is dispersal and decentral

Low power

Optical system—scattergram
 Forward light assembly problem

Content
 Clinics
 Overview
 Principle
 Installation
 Software
 Hardware
 Optical system
 Liquid system

Fluidic system—overview
 2 Analysis channel ： WBC&HGB channel, RBC&PLT channel

Sample
RBC/PLT WBC/HGB
M-52D diluent
M-52 DIFF lyse M-52 LH lyse
Impedance
Laser scattering Laser scattering
\colorimetry
RBC/PLT/MCV Lymph 、 Mon 、 Eos 、 N

eu WBC/BASO/HGB

Flowchart
（ WB-CBC+DIFF ）

Peripheral Blood
Sample 20μl
Diluent 480μl
Sample Diluent
Ratio 1:25 500μl
Outside the analyzer
Inside the analyzer Aspirate 200 μl of sample
Flowchart Diluent Background

358μl
WBC
bath
（ PD-CBC+DIFF ） Aspirate 21.6 μl of

Sample Diluent secondary sample
Ratio 1:69.75
Variable temperature control
WBC
bath DIFF Lyse 1ml
Aspirate 380 μl of mixed fluid 36°
Sample WBC RBC

preparation LH Lyse 200μl Diluent 2.4ml
bath bath
tube
WBC DIFF Sample WBC BASO Sample RBC Sample Diluent

Diluent Ratio 1:199.8 Diluent Ratio 1:234.3 Ratio 1:7750
Flow cell WBC bath

DIFF HGB RBC
Measurement Measurement bath
RBC/PLT
Sample preparation tube, flow cell cleaning Measurement
Flow cell
WBC/BASO
Measurement
Fluidic system—reagent consumption
Diluent DIFF lyse(ml) LH lyse(ml) Probe

Mode (ml) cleanser
（ ml ）
CD 27.5 1 0.2 0
WB
CBC 23.2 0 0.2 0
CD 27.5 1 0.2 0
Capillary_W
CBC 23.2 0 0.2 0
CD 27.6 1 0.2 0
Prediluted
CBC 22.9 0 0.2 0
Shutdown 60.9 1 0.2 2
Normal startup 65 1 0.2 0
Wake-up 1 3.7 0 0 0
Wake-up2 16.9 0 0 0
Wake-up3 65 3 0.2 0

Fluidic system—fluidic diagram
1 2 3 4 5 6
MRSZ/R05N01.291.02（2.0） REV ECN DESCRIPTION DESIGN
Add the information of LF, T76 and tubes in the optical assembly, Revise T3,
2.0 CR-3107-042 T10, T11, T18, T20, T22, T32, T44, T57, T66, T70, T71, T75, C18, C19, Yang Yijie
C20, C21
3.0 CR-3107-047 Add T80,J32 and revise T48,J15 Yang Yijie
SV08 C17 LF C22 C23

T19 T76 T20
A C14 DILUENT
T25 T49
CV1
T21 J29 J7-T31-J8 T30
SV05 C15
DP1 T50
Preheat SV01
Bath T32
T26
T24
C27
J14-T48-J15-T80-J32 T47
T17
T77
SV02 DP2
J30-T78-J31
WBC&HGB channel T18
Case
LH Reagent Bottle
T79 SV07 C18
C19
T29
T71
J9
RBC channel
SV04
WBC RBC
T51
PV18
J25-T23-J26
B
C11 C10
J20-T63-J21 T34 T52
Case T33 C6
J22-T64-J23-T65-J24
J12-T45-J13
T72
Temperature Sensor T75
J10-T35-J11
T54
J16-T59-J17
J18-T60-J19
C20
SV12
Isolation Chamber 1
Isolation Chamber 2
C21
T3
SV16 SV14 C12 C13
DIFF Reagent Bottle

T61 T62
SV06
T70
C5 SV13
T9
C4 T12 T13 T14 T37 T38

T39
C9
T40
T55
T15
T36
C3 SV11
T11 T16 C7
SV15
C26
T41
Hydraulic J5-T7-J6-T8
T10
C Sensor T53
C2 T46 C16
J4-T6 C8
Transducer
J3-T5
2 T57 T58
T56
T42
T
J27-T22-J28
SV10 T67
C1 SV03
T43 T66
1
Sampling/dispensing
T
SPB
J1-T4-J2
Diluent Tank
T73
VC
T74
SV09
T44
WASTE LP
Waste
Tank C25 C24
T69 T68 Confidential classification:
Confidential
SR CONFIDENTIAL
D
TITLE 3107 Pneumatic & Fluidics System Diagram
保密:此图及其全部知识产权(含著作权)归深圳迈瑞生物医疗电子股份有限公司所有。未经深圳迈瑞生物医疗电子股份有限公司预先书面许可,严禁出于任何目的,对 DOC No. A1-115-015670-00
此图的全部或部分内容(包括但不限于图中信息、数据、运算结果等)泄露、使用、拷贝或复制。
Classified documents, This set of drawing(s) and all it's intellectual property rights (including copyright) subsisting herein are property of DESIGN 杨一杰 REV. 3.0
Shenzhen Mindray Bio-medical Electronics Co.,Ltd. No disclosure,use,copies or reproductions should be made of this drawing or any part(s)
thereof for whatever purpose nor shall any information, data, calculations, or other contents contained in this drawing be disseminated without P.CODE 3107 SIZE A4
prior written permission of Shenzhen Mindray Bio-medical Electronics Co.,Ltd Software &Rev:
SHEET 1 OF 6
Microsoft visio 2003

Fluidic system—component
 Highly integrated air and hydraulic system
 17valves ： 1 high pressure (2-way)(V3);1pinch valve(V18) 、 1LVM(V11)
 1 syringe assembly (2 syringes ， 250ul 、 10ml ）
 1 sampling assembly
 2 Electromagnetic Metering pumps （ 1ml 、 200ul ）
 2 counting baths
 2 isolate chambers
 1 flow cell
 1 waster pump
 1 preheat bath assembly
 1 hydraulic pressure sensor
 1 check valve
 1 filter
 1 vacuum chamber

 VALVE

 Syringe Linkage
Syringe Linkage consist of a motor, a

driving assembly and two syringes
Name Specification Function

Small Used for quantitative aspiration, distribution and
Full scale
volume secondary aspiration of blood samples, and for injecting the
250μL
syringe sample into the flow cell for measurement
Used for quantitative addition of diluent to WBC and
Large RBC bath, wipe fluid supply, cleaning of interior and
Full scale
volume exterior of sample probe and reaction bath, forcing the
10mL
syringe sheath into the flow cell, cleaning of the flow cell and
sample preparation.

 Electromagnetic  Hydraulic pressure sensor

Metering Pump
symbol
symbol
Function ： Function:
DP1 ： 1ml ， DIFF lyse adding Monitoring fluid pressure, give a
DP2 ： 200ul ， LH lyse adding alert signal when abnormal or
beyond settings.
 Preheat bath  Tem. Sensor for diluent
symbol symbol
To heat DIFF Monitor Temp of dluent,

lyse ， maintain the Temp recommend a target Temp to
in DIFF reaction bath(WBC preheat bath
bath)
 Fluidic temperature control
Item Preheat bath diluent

Target Refer to diluent
Temp
Alarm <target Temp -1.5 <10, >40
Temp >target Temp +3
Diluent
Temperature
Sensor
Reaction Bath
Temperature
J10 Sensor
PIN1 Temperature
Temperature
Analog Drive Switch
Switch
Preheat
Preheat
J21 assembly
assembly
Board PIN3

 Waste pump  Check valve
drain the wipe block, WBC bath,

RBC bath and vacuum chamber, Define the flow direction of DIFF
create vacuum in the vacuum lyse in case of reverse flow
chamber Attention : direction ！

Fluidic system—component  Wipe block
 Sample probe
Aspirate and dispense the blood sample

and probe cleanser.
Note: the sample probe is flat-tipped
Clean the sample probe and drain out the
with a side opening to ensure normal
waste fluid while cleaning the inner and
aspiration in case that the tip touches
outer of the probe
the bottom of the sample tube.

 Count reaction bath

 New structure
 Easy to maintenance
 WBC bath ： for sample mixing ,reacting, HGB measuring, provide

diluted and reacted sample for DIFF\BASO counting
 RBC bath ：
 50um aperture(improve the accuracy of PLT)
 Special design to reduce bubble in back bath
 Consist of aperture, front bath and back bath
 RBC diluting and mix and counting

 HGB assembly
 New design
 Convergent optical path
 Easy to Maintenance

 Vacuum chamber ： maintain negative pressure for RBC counting. Same

with that of BC3000
 WBC/RBC Isolation chamber :provide an isolation air room to cut off the
internal or external interference

Cooperate with DP1 for DIFF Wipe block drainage ， vacuum
SV01 SV10
lyse suction /drainage pressure release
Cooperate with DP2 for LH Control syringe switch to sampling or
SV02 SV11
lyse suction/ drainage sample preparation route
 Valve function
On/off between large and mall RBC bath to vacuum chamber on/off
SV03 SV12
syringe control for RBC counting
SV04 On/off the sheath flow SV13 RBC drainage
Control diluent adding to RBC Rinse sample preparation pipe ,RBC
SV05 SV14
bath bubbling
SV06 Bubbling for WBC bath SV15 WBC drainage
RBC bath rinse, cooperate with
SV07 SV16 Floe cell drainage
SV09 to rinse the wipe block
Control syringe suction of
diluent, cooperate with Pinch valve for sample preparation,
SV08 SV7/sv9 in dispensing diluent PV18 cooperate with syringe controlling
to RBC back bath and wipe sample preparation and rinse
block ，
cooperate with SV7 to rinse the
SV09
wipe block

Fluidic system—functioning and sequence
 Sampling/dispensing channel
SV08 C17 LF
T19 T76 T20
Function ：
Diluent
 Sampling 、 HGB
T24
T17
SV07
C3
Fluidic
pressure
SV11
RBC
measurement
T51
C26
 Rinse the outside of the

J5-T7-J6-T8
T10
sensor T53
C2
J4-T6 SV10 T52
SP
C6
J3-T5
T57
2
T
J27-T22-J28
C1 SV03
1
T
SPB
T58 T66
 Rinse the inside of the
J1-T4-J2
SV09
T43 SP
T44
VC
 Suck and dispense
SR Waste
LP probe cleanser
T68

Function ：
SV08 C17 LF
T19 T76 T20
diluent measurement
T24
T17
C3 RBC
SV07
SP
SV11
T51
C26 J5-T7-J6-T8
T10
T53
C2
J4-T6 SV10 T52
C6
SP
J3-T5
T57
2
T
J27-T22-J28
C1 SV03
1
T
SPB
T58 T66
J1-T4-J2
SV09
T43 probe cleanser
VC
T44
LP
SR
T68

Function ：
SV08
T19
C17
T76
LF
T20  Sampling 、 HGB
diluent measurement
T24
 Rinse the outside of
T17
SV07
C3 RBC
SV11
the SP
T51
C26 J5-T7-J6-T8
T10

T53
C2
J4-T6 SV10 T52
C6
SP
J3-T5
T57
2
T
J27-T22-J28
C1 SV03
1
T
SPB
T66
T58
J1-T4-J2
SV09
T43 probe cleanser
VC
T44
LP
SR
T68

SV08 C17 LF
T19 T76 T20
diluent
T24
Function ：
T17
SV07
C3 RBC
SV11
T51
C26 J5-T7-J6-T8
T10
measurement
T53
C2
J4-T6 SV10 T52

C6
J3-T5
T57
2
T
J27-T22-J28
C1 SV03
1
T
SPB
T66
SP
T58
J1-T4-J2
T43
SV09
T44
VC SP
LP
SR
T68 probe cleanser

 WBC&HGB channel
SV08 C17 LF C14
T19 T76 T20 T25 T49
CV1
diluent J29 J7-T31-J8 T30
DIFF lyse
DP1
C27 Preheating T32
SV01
chamber C15
T77
J30-T78-J31
T50
T26
T9 LH lyse
J14-T48-J15-T80-J32 T47
T17
SV02 DP2
SV04
WBC
Function ：
J9
PV18
J25-T23-J26
J20-T63-J21
C11
外套
T34
C10
T33
 Inject 240ul diluent
J12-T45-J13
T75
J10-T35-J11
 Dispense 15ul blood +

J22-T64-J23-T65-J24
J16-T59-J17
diluent 312ul
SV16 SV14 C12 C13
T61 T62
SV06
C5
T9
C4 T12 T13 T14 T37 T38

C3
T11
SV11
C9
 Suck diluted sample 12ul
T15
T36
C26
T10
 Add 1ml DIFF lyse

T53 T16 C7
C2 J5-T7-J6-T8
SV15
J4-T6
T41
 Bubble mixing
J3-T5
2
T T46
C8
J27-T22-J28
C1 SV03
T42
1
T
T66
SPB
J1-T4-J2
VC
Waste LP
SR T68
T69

 WBC&HGB channel
SV08 C17 LF C14
T19 T76 T20 T25 T49
CV1
J29 J7-T31-J8 T30
diluent DIFF lyse
DP1
C27 Perheating SV01
T32
chamber
C15
T77
J30-T78-J31
T50
T26
T79
J14-T48-J15-T80-J32 T47 LH lyse
T17
SV02 DP2
SV04
WBC
J9
PV18
J25-T23-J26
J20-T63-J21
C11
T34
C10 Function ：
T33
 Sample preparation 380ul

J12-T45-J13
T75
J10-T35-J11
J22-T64-J23-T65-J24
J16-T59-J17  Adjust the position in the

SV16 SV14 C12 C13
T61 T62
SV06
C5
T9
tube
C4 T12 T13 T14 T37 T38
C3 SV11
T11 C9
T15
T36
Fluidic
C26
 Add 200ul LH lyse

T10
pressure T53 T16 C7

C2 sensor J5-T7-J6-T8
SV15
J4-T6
T41
 Bubble mixing
J3-T5
2
T T46
C8
J27-T22-J28
C1 SV03
T42
1
T
T66
SPB
J1-T4-J2
VC
Waste LP
SR T68
T69

 WBC&HGB channel
SV08 C17 LF C14
T19 T76 T20 T25 T49
CV1
diluent J29 J7-T31-J8 T30 DIFF lyse
DP1
C27 Preheating SV01
T32
chamber
C15
T77
J30-T78-J31
T50
T79
T26
LH lyse
J14-T48-J15-T80-J32 T47
T17
SV02 DP2
SV04
WBC
Function ：
J9
PV18
J25-T23-J26
J20-T63-J21
C11
T75
T34
C10
T33
 To form the sheath flow
J12-T45-J13
J10-T35-J11
 To boost the sample

J22-T64-J23-T65-J24
J16-T59-J17
SV16 SV14 C12 C13
 DIFF count
T61 T62
SV06
C5
T9
C4 T12 T13 T14 T37 T38

C3
T11
SV11
C9
 HGB measurement
T15
T36
Fluidic
C26
T10
pressure T53 T16 C7

SV15
J4-T6
T41
J3-T5
2
T T46
C8
J27-T22-J28
C1 SV03
T42
1
T
T66
SPB
J1-T4-J2
VC
Waste LP
SR T68
T69

 WBC&HGB channel
SV08 C17 LF C14
T19 T76 T20 T25 T49
CV1
diluent J29 J7-T31-J8 T30 DIFF lyse
DP1
C27 Preheating SV01
T32
chamber
C15
T77
J30-T78-J31
T50
外套
T26
T79 LH lyse
J14-T48-J15-T80-J32 T47
T17
SV02 DP2
SV04
WBC
Function ：
J9
PV18
J25-T23-J26
J20-T63-J21
C11
外套
T34
C10
T33
 Suck 156.3ul sample
J12-T45-J13
from WBC bath

T75
J10-T35-J11
J22-T64-J23-T65-J24
J16-T59-J17
 Rinse the flow cell
SV16 SV14 C12 C13
T61 隔隔 T62
SV06 离离
C5 室室
T9
C4 T12 T13 T14 1 2 T37 T38

C3
T11
SV11
C9
 Rinse the route of sample
T15
T36
C26
T10
preparation anf flush the

液压传感器 T53 T16 C7
C2 J5-T7-J6-T8
SV15
J4-T6
T41
J3-T5
2
T46
sample injector and flow

T
C8
J27-T22-J28
C1 SV03
T42
cell
1
T
T66
SPB
J1-T4-J2
VC
废液 LP
SR T68
T69

 WBC&HGB channel
SV08
T19
C17
T76
LF
T20
CV1
T25
C14
T49 Function ：
DIFF lyse
 Sample preparation
J29 J7-T31-J8 T30
diluent
Preheating DP1
C27 SV01
chamber T32
C15
800ul
T77
J30-T78-J31
T50
T79
T26
LH lyse
J14-T48-J15-T80-J32 T47
 To form sheath flow
T17
SV02 DP2
SV04
 To boost the sample

WBC
J9
PV18
J25-T23-J26
C11
 BASO counting
J20-T63-J21 C10
T34
T33
T75
J12-T45-J13
J10-T35-J11
J22-T64-J23-T65-J24
J16-T59-J17
SV16 SV14 C12 C13
 Rinse(same as DIFF)
 WBC bath drainage
T61 T62
SV06
C5
T9
C4 T12 T13 T14 T37 T38
 Inject diluent to WBC

C3 SV11
T11 C9
T15
T36
Fluidic
pressure C26
T10
T53 T16 C7
bath
sensor J5-T7-J6-T8
C2 SV15
J4-T6
T41
J3-T5
2
T T46
C8
J27-T22-J28
C1 SV03
T42
1
T
T66
SPB
J1-T4-J2
VC
Waste LP
SR T68
T69

 WBC&HGB channel
SV08 C17 LF C14
T19 T76 T20
diluent J29
CV1
J7-T31-J8
T25
T30
T49
DIFF lyse
Function ：
 Rinse (as DIFF)
DP1
C27 Reheating SV01
T32
chamber
C15
T77
J30-T78-J31
 Rinse and drain out
T50
T79
T26
LH lyse
J14-T48-J15-T80-J32 T47
T17
SV04
WBC
SV02 DP2
WBC bath
 Inject 2018ul diluent to
J9
PV18
J25-T23-J26
C11
WBC bath as base

J20-T63-J21 C10
T34
T33
T75
J12-T45-J13
J10-T35-J11
J22-T64-J23-T65-J24
J16-T59-J17
SV16 SV14 C12 C13
solutioon
T61 T62
SV06
C5
T9
C4 T12 T13 T14 T37 T38

C3 SV11
T11 C9
T15
T36
Fluidic
C26
T10
pressure T53 T16 C7

SV15
J4-T6
T41
J3-T5
2
T T46
C8
J27-T22-J28
C1 SV03
T42
1
T
T66
SPB
J1-T4-J2
VC
Waste LP
SR T68
T69

 RBC&PLT channel SV08 LF

T19 C17
Fuction ：
T76 T20
diluent
T21
SV05
 Inject diluent (1600ul)
T18
T24
SV07
 Add diluted sample
T29
T17
RBC
T51
(12ul) to RBC bath
 Bubble mixing
T52
C6
Temp. sensor J20-T63-J21 C11
T54
J18-T60-J19
 RBC 、 PLT counting
J10-T35-J11
SV12
J22-T64-J23-T65-J24
T3
C13
T62
SV14 SV13
C4 T37 T38
T39
C9
T40
T55
C3 SV11
T11 T36 C7
Fluidic T43
C26
T41
pressure J5-T7-J6-T8
T10
T53
sensor C16
C2
J4-T6 C8
Transducer
J3-T5
T56
T T42
J27-T22-J28
T67
C1 SV03 T66
1
T
SPB
J1-T4-J2
VC
SV09
T44 LP
Waste
T69 T68
SR

 RBC&PLT channel
SV08 C17 LF
T19
T76 T20
diluent
T21
Function ：
SV05
 Rinse the RBC

T18
T24
SV07
T29
front bath
T17
RBC
T51
T52
C6
Temp.sensor J20-T63-J21 C11
T54
J18-T60-J19
J10-T35-J11
SV12
J22-T64-J23-T65-J24
T3
C13
T62
SV14 SV13
C4 T37 T38
T39
C9
T55
T40
C3 SV11
T11 T36 C7
Fluidic T43
C26
T41
pressure J5-T7-J6-T8
T10
sensor T53
C2 C16
J4-T6 C8
Transducer
J3-T5
T56
T T42
J27-T22-J28
T67
C1 SV03 T66
1
T
SPB
J1-T4-J2
VC
SV09
T44 LP
Waste
T69 T68
SR

 RBC&PLT channel
SV08 C17 LF
Function ：
T19 T76 T20
diluent
 Rinse the RBC
back bath
T24
T17
SV07
C3
Fluidic
SV11
RBC
 Inject diluent
T51
(2560ul) to RBC
pressure C26 J5-T7-J6-T8
T10
sensor T53
C2
J4-T6 SV10 T52
C6
bath as base
J3-T5
T57
2
T
J27-T22-J28
C1 SV03
1
T
SPB
solution
T66
 Release vacuum
T58
J1-T4-J2
T43
SV09
VC
T44
LP
SR Waste
T68

Fluidic system—sequence in detail
 WB_CBC+DIFF
SV08 C17 LF C22 C23
T19 T76 T20
C14 DILUENT
T25 T49
CV1
Small syringe SV05

T21 J29 J7-T31-J8 T30
DP1
C15
T50
Preheat SV01
aspirate 15μL blood Bath T32
T26
T24
C27
J14-T48-J15-T80-J32 T47
sample, while large
T17
T77
SV02 DP2
J30-T78-J31
T18
Case
syringe aspirate 600ul
LH Reagent Bottle
T79 SV07 C18
C19
T29
T71
diluent,
J9
SV04
WBC RBC
T51
HGB background
PV18
J25-T23-J26
Empty WBC bath

C11 C10
J20-T63-J21 T34 T52
Case T33 C6
J22-T64-J23-T65-J24
J12-T45-J13
T72
J10-T35-J11
Linkage SR aspirate
T54
J16-T59-J17
J18-T60-J19
C20
Isolation Chamber 2
Isolation Chamber 1
SV12
C21
DIFF Reagent Bottle

5360ul diluent
T3
SV16 SV14 C12 C13

T61 T62
SV06
T70
(SV03 、 PV18, SV11
C5 SV13
T9
C4 T12 T13 T14 T37 T38

T39
C9
T40
T55
T15
toT64 ）
T36
C3 SV11
T11 T16 C7
Zap aperture of RBC

SV15
C26
T41
T10
Sensor T53
C2 T46 C16
J4-T6 C8
Transducer
T57
J3-T5
T58
2
T56
T42
T
J27-T22-J28
SV10 T67
C1 SV03
T43 T66
1
T
SPB
J1-T4-J2
Diluent Tank
T73
VC
T74
SV09
T44
WASTE LP
Waste
Tank C25 C24
T69 T68
SR

SV08 C17 LF C22 C23
T19 T76 T20
C14 DILUENT
T25 T49
CV1
T21 J29 J7-T31-J8 T30
SV05 C15
DP1 T50
Preheat SV01
T32
 Sample probe goes up

Bath
T26
T24
C27
J14-T48-J15-T80-J32 T47
T17
to upper position( the
T77
SV02 DP2
J30-T78-J31
T18
Case
LH Reagent Bottle
T79 SV07 C18
tip inside the wipe) C19
T29
T71
J9
SV04
WBC RBC
 Linkage SR rinse
T51
PV18
J25-T23-J26
exterior of SPB and J20-T63-J21

C11
Case
T34
C10
T33
T52
C6
J22-T64-J23-T65-J24
J12-T45-J13
T72
T75
RBC back bath, drain

Temperature Sensor
J10-T35-J11
T54
J16-T59-J17
J18-T60-J19
C20
Isolation Chamber 1
Isolation Chamber 2
SV12
by LP
C21
DIFF Reagent Bottle

T3
SV16 SV14 C12 C13

T61 T62
SV06
T70
C5 SV13
T9
T12 T13 T14 T37 T38
 SPB to the position

C4
T39
C9
T40
T55
T15
T36
above the WBC bath

C3 SV11
T11 T16 C7
SV15
C26
T41
T10
Sensor T53
C2 T46 C16
 Empty WBC bath for J4-T6 C8

Transducer
J3-T5
2 T57 T58
T56
T42
T
J27-T22-J28
T67
the second time

C1 SV03 SV10
T43 T66
1
T
SPB
J1-T4-J2
Diluent Tank
T73
VC
T74
SV09
T44
WASTE LP
Waste
Tank C25 C24
T69 T68
SR

Fluidic system—sequence in detailv
SV08 C17 LF C22 C23
T19 T76 T20
C14 DILUENT
T25 T49
CV1
T21 J29 J7-T31-J8 T30
Linkage SR injects
SV05 C15
DP1 T50
Preheat SV01
Bath T32
T26
T24
240ul diluent into WBC
C27
J14-T48-J15-T80-J32 T47
T17
T77
SV02 DP2
J30-T78-J31
bath as base solution
T18
Case
T79
LH Reagent Bottle
SV07 C18
Empty RBC bath

C19
T29
T71
J9
SV04
SPB goes down

WBC RBC
T51
PV18
J25-T23-J26
into WBC bath J20-T63-J21

C11
Case
T34
C10
T33
T52
C6
J22-T64-J23-T65-J24
Small syringe
J12-T45-J13
T72
J10-T35-J11
T54
J16-T59-J17
J18-T60-J19
C20
Isolation Chamber 1
Isolation Chamber 2
SV12
dispenses 18 sample,
C21
DIFF Reagent Bottle

T3
SV16 SV14 C12 C13

T61 T62
SV06
T70
large SR rinse RBC C5 SV13
T9
C4 T12 T13 T14 T37 T38

T39
inlet with 720ul diluent

C9
T40
T55
T15
T36
C3 SV11
T11 T16 C7
Linkage SR inject C26

SV15
T41
T10
Sensor T53
C2 T46 C16
312ul rinse interior of

J4-T6 C8
Transducer
J3-T5
2 T57 T58
T56
T42
T
J27-T22-J28
T67
SPB(SV03,
C1 SV03 SV10
T43 T66
1
T
SPB
SV11),sample diluted
J1-T4-J2
Diluent Tank
T73
VC
T74
SV09
T44
WASTE LP
Waste
Tank C25 C24
T69 T68
SR

 WBC bubble mixing, SV08

T19
C17
T76
LF
T20
C22 C23
linkage SR T21 J29

CV1
J7-T31-J8
T25
T30
C14
T49
DILUENT
SV06(SV03, SV11, SV05

Preheat
Bath T32
SV01
DP1
C15
T50
T26
PV18)
T24
C27
J14-T48-J15-T80-J32 T47
T17
T77
SV02 DP2
J30-T78-J31
T18
 SPB goes up (inside the
Case
T79
LH Reagent Bottle
SV07 C18
C19
T29
T71
wipe), LP drain the waste
J9
SV04
WBC RBC
T51
PV18
of wipe block
J25-T23-J26
C11 C10
J20-T63-J21 T34 T52
Case T33 C6
 Rinse interior of SPB, ),
J22-T64-J23-T65-J24
J12-T45-J13
T72
J10-T35-J11
T54
J16-T59-J17
J18-T60-J19
C20
LP drain the waste of

SV12
Isolation Chamber 2
Isolation Chamber 1
C21
DIFF Reagent Bottle

T3
SV16 SV14 C12 C13

T61 T62
T70
SV06
wipe block C5 SV13

T9
C4 T12 T13 T14 T37 T38

T39
C9
T40
T55
T15
T36
 Linkage SR aspirate C3
T11
C26
SV11
SV15
T16 C7
T41
T10
1580ul diluent. Sensor T53

C2 T46 C16
J4-T6 C8
Transducer
J3-T5
2 T57 T58
T56
T42
T
J27-T22-J28
 SPB down into WBC

SV10 T67
C1 SV03
T43 T66
1
T
SPB
bath
J1-T4-J2
Diluent Tank
T73
VC
T74
SV09
 DP1 suck DIFF lyse T44 Waste

Tank
WASTE
C25 C24
LP
T69 T68
SR

 Empty RBC bath

SV08 C17 LF C22 C23
T19 T76 T20
C14 DILUENT
T25 T49
 Small SPB aspirate

CV1
T21 J29 J7-T31-J8 T30
SV05 C15
DP1 T50
12ul diluted sample

Preheat SV01
Bath T32
T26
T24
C27
J14-T48-J15-T80-J32 T47
from WBC bath, large

T17
T77
SV02 DP2
J30-T78-J31
T18
Case
SPB for 480 diluent T79 SV07 C18
LH Reagent Bottle
C19
T29
T71
(SV03 off)
J9
SV04
WBC RBC
T51
PV18
J25-T23-J26
 SPB goes up (inside J20-T63-J21

C11
T34
C10
T52
Case T33 C6
J22-T64-J23-T65-J24
the wipe),rinse exterior
J12-T45-J13
T72
J10-T35-J11
T54
J16-T59-J17
J18-T60-J19
C20
of SPB LP drain the

SV12
Isolation Chamber 1
Isolation Chamber 1
C21
DIFF Reagent Bottle

T3
SV16 SV14 C12 C13

T61 T62
waste of wipe block,

SV06
T70
C5 SV13
T9
C4 T12 T13 T14 T37 T38

T39
then turn to RBC bath.

C9
T40
T55
T15
T36
C3 SV11
T11 T16 C7
SV15
 DP1 add DIFF lyse to

C26
T41
T10
Sensor T53
C2 T46 C16
J4-T6 C8
Transducer
WBC bath
J3-T5
2 T57 T58
T56
T42
T
J27-T22-J28
SV10 T67
C1 SV03
T43 T66
 Bubble mix WBC via

1
T
SPB
J1-T4-J2
Diluent Tank
T73
VC
SV06.
T74
SV09
T44
WASTE LP
Waste
Tank C25 C24
T69 T68
SR

SV08 C17 LF C22 C23
T19 T76 T20
C14 DILUENT
T25 T49
CV1
T21 J29 J7-T31-J8 T30
SV05 C15
DP1 T50
Preheat SV01
Bath T32
T26
T24
C27
J14-T48-J15-T80-J32 T47
T17
 Linkage SR add1600ul
T77
SV02 DP2
J30-T78-J31
T18
Case
diluent via SV05 to

T79
LH Reagent Bottle
SV07 C18
C19
T29
T71
J9
RBC bath as base SV04
WBC RBC
T51
PV18
solution
J25-T23-J26
C11 C10
J20-T63-J21 T52
T34
 SPB down to RBC

Case T33 C6
J12-T45-J13
T72
J22-T64-J23-T65-J24
J10-T35-J11
T54
J16-T59-J17
J18-T60-J19
C20
SV12
Isolation Chamber 1
Isolation Chamber 2
 SPB dispense 12ul
C21
DIFF Reagent Bottle

T3
SV16 SV14 C12 C13

T61 T62
SV06
T70
diluted sample + 800ul C5 SV13
T9
C4 T12 T13 T14 T37 T38

T39
C9
diluent (rinse interior of
T40
T55
T15
T36
C3 SV11
T11 T16 C7
SPB) C26
SV15
T41
T10
Sensor T53
C2 T46 C16
J4-T6 C8
Transducer
SPB to upper position LP
J3-T5
2 T57 T58
T56
T42
T
J27-T22-J28
SV10 T67
C1 SV03
T43 T66
drain waste of wipe block 1

T
SPB
J1-T4-J2
Diluent Tank
T73
VC
T74
SV09
T44
WASTE LP
Waste
Tank C25 C24
T69 T68
SR

SV08 C17 LF C22 C23
T19 T76 T20
C14 DILUENT
T25 T49
CV1
T21 J29 J7-T31-J8 T30
SV05 C15
DP1 T50
Preheat SV01
Bath T32
T26
T24
J14-T48-J15-T80-J32 T47
T17
T77
SV02 DP2
J30-T78-J31
diluent from diluent tank
T18
Case
T79 C18
LH Reagent Bottle
SV07
C19
T29
 SPB to the up position of
T71
J9
SV04
WBC RBC
T51
sampling
PV18
J25-T23-J26
C11 C10
J20-T63-J21
T34 T52
 Linkage SR aspirate Case T33 C6
J12-T45-J13
T72
J22-T64-J23-T65-J24
J10-T35-J11
T54
J16-T59-J17
J18-T60-J19
380ul DIFF sample into SV12
C20
Isolation Chamber 1
Isolation Chamber 2
C21
DIFF Reagent Bottle

T3
SV16 SV14 C12 C13
sample preparation
T61 T62
T70
SV06
C5 SV13
T9
C4 T12 T13 T14 T37 T38
pipe(T65-T63), C9
T39
T40
T55
T15
T36
C3 SV11
T11 T16 C7
 DP2 suck LH lyse C26

SV15
T41
T10
Sensor T53
C2 T46 C16
J4-T6 C8
 Aspirate 200ul via C11-

Transducer
J3-T5
2 T57 T58
T56
T42
T
J27-T22-J28
SV10 T67
C1 SV03
T43 T66
T35 –SV14-ISC1, adjust 1

T
SPB
J1-T4-J2
sample position
Diluent Tank
T73
VC
T74
SV09
T44
WASTE LP
Waste
Tank C25 C24
T69 T68
SR

 Add LH lyse to WBC bath SV08

T19
C17
T76
LF
T20
C22 C23
DILUENT
 WBC bubble mixing
C14
T25 T49
CV1
T21 J29 J7-T31-J8 T30
SV05 C15
DP1 T50
 Large SR inject diluent to

Preheat SV01
Bath T32
T26
T24
C27
J14-T48-J15-T80-J32 T47
form sheath flow via

T17
T77
SV02 DP2
J30-T78-J31
T18
Case
SV04,
LH Reagent Bottle
T79 SV07 C18
C19
T29
T71
J9
 At the same time, SV03 SV04
WBC RBC
T51
PV18
J25-T23-J26
open 0.2s, linkage SR J20-T63-J21

C11
T34
C10
T52
boost sample to flow cell

Case T33 C6
J12-T45-J13
T72
J22-T64-J23-T65-J24
J10-T35-J11
T54
J16-T59-J17
J18-T60-J19
C20
Isolation Chamber 1
Isolation Chamber 2
SV12
 SV03 closed, small SR

C21
DIFF Reagent Bottle

T3
SV16 SV14 C12 C13

T61 T62
SV06
T70
push the sample pass C5 SV13
T9
C4 T12 T13 T14 T37 T38

T39
C9
through the flow cell,
T55
T40
T15
T36
C3 SV11
T11 T16 C7
sheath flow remains. C26

SV15
T41
T10
Sensor T53
C2 T46 C16
J4-T6 C8
Transducer
 DIFF detection for 9.1s
J3-T5
2 T57 T58
T56
T42
T
J27-T22-J28
SV10 T67
C1 SV03
T43 T66
 HGB measurement
1
T
SPB
J1-T4-J2
Diluent Tank
T73
VC
T74
SV09
T44
WASTE LP
Waste
Tank C25 C24
T69 T68
SR

SV08 C17 LF C22 C23
 Linkage SR pull down, C14

T19 T76 T20
DILUENT
large SR aspirate diluent,

T25 T49
CV1
T21 J29 J7-T31-J8 T30
SV05 C15
small SR aspirate 156.3ul Preheat

Bath T32
SV01
DP1 T50
T26
T24
sample from WBC bath
C27
J14-T48-J15-T80-J32 T47
T17
T77
SV02 DP2
J30-T78-J31
T18
Case
 Create negative pressure T79 SV07 C18
LH Reagent Bottle
C19
T29
in VC
T71
J9
SV04
WBC RBC
T51
PV18
J25-T23-J26
 Rinse flow cell via SV04- J20-T63-J21

C11 C10
T34 T52
SV16. rinse injector via Case T33 C6
J12-T45-J13
T72
J22-T64-J23-T65-J24
J10-T35-J11
T54
J16-T59-J17
J18-T60-J19
SV04-sv14. rinse sample C20
Isolation Chamber 1
Isolation Chamber 2
SV12
C21
DIFF Reagent Bottle

T3
SV16 SV14 C12 C13
preparation route via SV06

T61 T62
T70
C5 SV13
T9
SV11-SV14-isolation
C4 T12 T13 T14 T37 T38
T39
C9
T55
T40
T15
T36
chamber2 to bubble mix C3

T11
SV11
SV15
T16 C7
the sample in RBC

C26
T41
T10
Sensor T53
C2 T46 C16
J4-T6 C8
Transducer
J3-T5
T57
 The constant current

T58
2
T56
T42
T
J27-T22-J28
SV10 T67
C1 SV03
T43 T66
source of RBC bath turns

1
T
SPB
J1-T4-J2
Diluent Tank
ON
T73
VC
T74
SV09
WASTE
 RBC counting(SV12 on)
T44 LP
Waste
Tank C25 C24
T69 T68
SR

SV08 C17 LF C22 C23
T19 T76 T20
Aspirate rest sample into CV1

T25
C14
T49
DILUENT
preparation pipe(T63-T65) SV05

T21
Preheat
J29 J7-T31-J8 T30
SV01
DP1
C15
T50
from WBC Bath T32
T26
T24
C27
J14-T48-J15-T80-J32 T47
T17
T77
 Large SR inject diluent to
SV02 DP2
J30-T78-J31
T18
Case
LH Reagent Bottle
T79 SV07 C18
form sheath flow via SV04, C19
T29
T71
J9
SV04
RBC
 At the same time, SV03

WBC
T51
PV18
J25-T23-J26
open 0.2s, linkage SR boost J20-T63-J21

C11
Case
T34
C10
T33
T52
C6
sample to flow cell
J12-T45-J13
T72
J22-T64-J23-T65-J24
J10-T35-J11
T54
J16-T59-J17
J18-T60-J19
C20
Isolation Chamber 1
Isolation Chamber 2
SV12
C21
DIFF Reagent Bottle

 SV03 closed, small SR
T3
SV16 SV14 C12 C13

T61 T62
SV06
T70
C5 SV13
push the sample pass

T9
C4 T12 T13 T14 T37 T38

T39
C9
T40
T55
T15
through the flow cell,
T36
C3 SV11
T11 T16 C7
sheath flow remains

SV15
C26
T41
T10
Sensor T53
C2 T46 C16
J4-T6 C8
Transducer
 WBC /BASO counting
J3-T5
2 T57 T58
T56
T42
T
J27-T22-J28
SV10 T67
C1 SV03
T43 T66
1
T
SPB
J1-T4-J2
Diluent Tank
T73
VC
T74
SV09
T44
WASTE LP
Waste
Tank C25 C24
T69 T68
SR

39.8~48.8s RBC 、 PLT 计数
SV08 C17 LF C22 C23
T19 T76 T20
C14 DILUENT
T25 T49
CV1
T21 J29 J7-T31-J8 T30
SV05 C15
DP1 T50
Preheat SV01
Bath T32
T26
T24
C27
J14-T48-J15-T80-J32 T47
T17
T77
SV02 DP2
J30-T78-J31
T18
Case
 Empty RBC bath T79 SV07 C18
LH Reagent Bottle
C19
T29
T71
J9
 Rinse sample preparation SV04
WBC RBC
T51
PV18
J25-T23-J26
pipe via (PV18 power on) J20-T63-J21

C11
T34
C10
T52
SV03-SV11-C11- Case T33 C6
J12-T45-J13
T72
J22-T64-J23-T65-J24
J10-T35-J11
T54
J16-T59-J17
J18-T60-J19
SV14 、 rinse flow cell
Isolation Chamber 1
Isolation Chamber 2
C20
SV12
DIFF Reagent Bottle

C21
T3
SV16 SV14 C12 C13
via sv04-sv16 SV06

T61 T62
T70
C5 SV13
T9
C4 T12 T13 T14 T37 T38

T39
T40
T55
T15
T36
C3 SV11
diluent.
T11 T16 C7
SV15
C26
T41
T10
Sensor T53
C2 T46 C16
J4-T6 C8
Transducer
J3-T5
2 T57 T58
T56
T42
T
J27-T22-J28
SV10 T67
C1 SV03
T43 T66
1
T
SPB
J1-T4-J2
Diluent Tank
T73
VC
T74
SV09
T44
WASTE LP
Waste
Tank C25 C24
T69 T68
SR

SV08 C17 LF C22 C23
T19 T76 T20
C14 DILUENT
T25 T49
CV1
T21 J29 J7-T31-J8 T30
SV05 C15
DP1 T50
Preheat SV01
Bath T32
T26
T24
C27
J14-T48-J15-T80-J32 T47
T17
T77
SV02 DP2
J30-T78-J31
 Empty WBC bath
T18
Case
LH Reagent Bottle
T79 SV07 C18
C19
T29
T71
 Flush injector via SV04-
J9
SV04
WBC RBC
T51
PV18
C11-SV14-ISC J25-T23-J26
C11 C10
J20-T63-J21 T34 T52
 Rinse pinch tube to WBC Case T33 C6
J12-T45-J13
T72
J22-T64-J23-T65-J24
J10-T35-J11
T54
J16-T59-J17
J18-T60-J19
(PV18 power off) C20
Isolation Chamber 1
Isolation Chamber 2
SV12
C21
DIFF Reagent Bottle

T3
SV16 SV14 C12 C13

T61 T62
 Empty WBC bath

SV06
T70
C5 SV13
T9
C4 T12 T13 T14 T37 T38

T39
C9
T40
T55
T15
T36
C3 SV11
T11 T16 C7
SV15
C26
T41
T10
Sensor T53
C2 T46 C16
J4-T6 C8
Transducer
J3-T5
2 T57 T58
T56
T42
T
J27-T22-J28
SV10 T67
C1 SV03
T43 T66
1
T
SPB
J1-T4-J2
Diluent Tank
T73
VC
T74
SV09
T44
WASTE LP
Waste
Tank C25 C24
T69 T68
SR

SV08 C17 LF C22 C23
T19 T76 T20
C14 DILUENT
T25 T49
 Add 2560ul diluent to RBC

CV1
T21 J29 J7-T31-J8 T30
SV05 C15
DP1 T50
bath
Preheat SV01
Bath T32
T26
T24
C27
J14-T48-J15-T80-J32 T47
T17
 Add 2018ul diluent to WBC
T77
SV02 DP2
J30-T78-J31
T18
Case
T79
LH Reagent Bottle
SV07 C18
bath C19
T29
T71
J9
SV04
RBC
 Turn on the LP rinse wipe

WBC
T51
PV18
J25-T23-J26
block J20-T63-J21
C11
Case
T34
C10
T33
T52
C6
J12-T45-J13
T72
J22-T64-J23-T65-J24
J10-T35-J11
T54
 SPB to ready position
J16-T59-J17
J18-T60-J19
C20
SV12
Isolation Chamber 1
Isolation Chamber 2
C21
DIFF Reagent Bottle

T3
SV16 SV14 C12 C13

T61 T62
 Small SR aspirate 4ul air to SV06
T70
C5 SV13
T9
C4 T12 T13 T14 T37 T38
create an isolation bubble

T39
C9
T40
T55
T15
T36
C3 SV11
T11 T16 C7
 Release vacuum pressure

SV15
C26
T41
T10
Sensor T53
C2 T46 C16
J4-T6 C8
(SV10 on, LP off) Transducer

J3-T5
2 T57 T58
T56
T42
T
J27-T22-J28
SV10 T67
C1 SV03
T43 T66
 Ready for next test. 1

T
SPB
J1-T4-J2
Diluent Tank
T73
VC
T74
SV09
T44
WASTE LP
Waste
Tank C25 C24
T69 T68
SR

 Measurement sequence in PD-CBC+DIFF mode

 Measurement sequence in PD-CBC+DIFF mode is basically the same
with the sequence in WB-CBC+DIFF mode, except that:
 Blood sample has been prediluted outside the analyzer,
 200μL of prediluted blood is aspirated in the PD mode
 Measurement sequence in CBC mode
 Compared with the sequence in CBC+DIFF mode, the measurement
sequence in CBC mode is basically the same except that:
 Sample volume: 15μL in the WB-CBC+DIFF mode, 11.7μL in the WB-
CBC mode.(but for PD mode, CBC+DIFF and CBC mode are the same )
 There is no actions related to DIFF measurement, including addition of
DIFF reagent, preparation of DIFF samples, DIFF measurement, and
cleaning process of the tubes and the flow cell after the DIFF measurement.

Fluidic system—sequence for maintenance
Maintenance with probe cleanser(shut down ） SV08 C17 LF C22 C23
T19 T76 T20
The orange lines are the CV1

T25
C14
T49
DILUENT
locations soaked by the

T21 J29 J7-T31-J8 T30
SV05 C15
DP1 T50
Preheat SV01
T32
probe cleanser,
Bath
T26
T24
C27
J14-T48-J15-T80-J32 T47
T17
T77
SV02 DP2
J30-T78-J31
the blue lines are the
T18
Case
LH Reagent Bottle
T79 SV07 C18
C19
locations passed by the
T29
T71
J9
SV04
probe cleanser
WBC RBC
T51
PV18
J25-T23-J26
C11
enhanced probe cleanser J20-T63-J21 C10

T34 T52
Case T33 C6
J22-T64-J23-T65-J24
J12-T45-J13
T72
J10-T35-J11
T54
maintenance sequence
J16-T59-J17
J18-T60-J19
C20
Isolation Chamber 1
Isolation Chamber 1
SV12
C21
DIFF Reagent Bottle

will be called every 300
T3
SV16 SV14 C12 C13

T61 T62
SV06
T70
times of sample
C5 SV13
T9
C4 T12 T13 T14 T37 T38

T39
C9
T40
T55
T15
measurement.
T36
C3 SV11
T11 T16 C7
The soaking time of

SV15
C26
T41
Hydraulic
T10
Sensor T53
C2 T46 C16
J5-T7-J6-T8 C8
enhanced maintenance is
J4-T6
T2 Transducer
J3-T5
T57 T58
T56
T42
J27-T22-J28
SV10 T67
6.67S
C1 SV03
T43 T66
T1
SPB
In the Maintenance
Diluent Tank
J1-T4-J2
T73
VC
screen, the probe
T74
SV09
T44
WASTE LP
cleanser maintenance is
Waste
Tank C25 C24
T69 T68
defaulted as enhanced SR
probe cleanser
maintenance.
Starting up rinse
 The fluidic actions on startup can be divided into three procedures: ：
1. Initialization of fluidics components: initialization of sampling assembly and
syringe assembly; 1 metering pump action (without consumption of reagents);
creating vacuum and releasing vacuum.
2. Cleanup: including cleaning of all the tubes and components on the analyzer;
removing bubbles from the flow cell; discarding 1 ml of DIFF reagent and 0.2 ml
of LH reagent.
3. Background: with WB-CBC+DIFF measurement sequence
If the background fails, perform the cleanup again and measure the
background.
For startup after an abnormal shutdown, perform the cleanup twice. 。
Initialization of fluidics components, cleaning up of the interior and exterior
surface of the sample probe while starting up after updating

Fluidic system—standby
 The instrument will enter standby status after idling for 15 to 30 minutes (defaulted as
15 minutes). After entering standby status, operations without fluidic actions can be
performed from the screen.
 Exit standby status 1: Standby for less than 1 hour.
Clean the exterior of the sample probe and the WBC bath and rebuild the
isolation bubble without consumption of lyse.
 Exit standby status 2: Standby for more than 1 hour and less than 5 hour.
 Clean the interior and exterior of the sample probe, the WBC bath, the RBC
bath, the sample supply tube and the flow cell, and rebuild the isolation bubble
without consumption of lyse.
 Exit standby status 3: Standby for not less than 5 hour.
The same as cleanup on startup, including cleaning of all the tubes and
components on the analyzer; removing bubbles from the flow cell; discarding 3 ml of
DIFF reagent to eliminate the effects of bubbles in the preheat bath tube; discarding
0.2 ml of LH reagent to eliminate the effects of crystal and bubbles.
？
目录
 Clinics
 Overview
 Principle
 Installation
 Software
 Hardware
 Optical system
 Liquid system

Mechanical system—overall layout
① 2-way valve
② 3-way valve
③ Syringe assemly
④ 1ml metering pump
⑤ microswitch
⑥ [aspirate] key
⑦ 200ulmetering pump
⑧ Sampling assembly
⑨ Fluidic pressure sensor
 Front view of the main unit (cover opened)

① Analog drive board

② Main control board
③ Fan assembly
 Back view of the main unit (internal structure)

① Optical system
② Front cover
③ Reagent baffle
④ LH lyse
⑤ DIFF lyse
⑥ Net 、 USB interface
⑦ Power supply assembly
 Left view of the main unit (left

door removed)
① Fluid inlet/outlet
② Waste pump
③ Vacuum chamber
④ valve
⑤ LVM valve
⑥ Diluent Temp. sensor
⑦ Sampling assembly
⑧ Front cover
⑨ RBC bath
⑩ WBC bath
 Right view of the main unit (right door removed)

① Bath cap
② Pan head screw M3X6
③ Bath holder
④ Bath adjust holder
⑤ RBC bath
⑥ Aperture (D50um)
⑦ Front bath washer
⑧ RBC back bath
 RBC bath subassembly

① Bath cap
② Pan head screw M3X6
③ Bath holder
④ Bath adjust holder
⑤ WBC bath
⑥ HGB assembly
 WBC bath assembly

Mechanical system—disassembling
RBC bath maintenance assembly

pipes are fixed to the holder

with cable tie
Winding pipe and

adapter should
be tied up with
cable tie
Replace with specific pipe and
surround with winding pipe as
illustration in the figure

pipes are fixed to the holder

with cable tie
Winding pipe and

adapter should
be tied up with
cable tie
Replace with specific pipe and
surround with winding pipe as
illustration in the figure

Disassembly rear plate of power supply

assembly

Mechanical system—adjustment of bath position

Mechanical system—adjustment of bath position
Quality Control
Operation
Operation content
Procedure QC Point QC Standard
Click on “Service → Sample Probe Debug”. Enter the
1.1
Debug screen as shown in Figure 1.1.
First, click on the “Initial position” so that the sample
1.2 probe is above the RBC bath, as shown in Figure 1.2.
Click on the “WBC bath”(or “RBC bath”) button shown in Fine adjust The sample
Figure 1.2. Click the “Middle position” button. Check if the the relative probe is
sample probe is basically in the center of the bath. If there position of basically in the
1.3 is any deviation, loosen the four screws shown in Figure the bath center of the
1.3 and slightly adjust the bath in left/right and front/back bath
directions so that the sample probe is in the center of the
bath. Then tighten one screw in each direction
Click on the “Initial position” button, then click “WBC Check if The sample
bath”(“RBC bath”) →“Down position” button. Check there the bath probe can not
is no interference with the bath as the sample probe position is contact with the
1.4 enters the bath. If the interference occurs, readjust the successfully bath 。
bath position in accordance with step 1-3 and repeat this adjusted
step to check. Finally, tighten the 4 screws.
Note:
In Figure 1.1, all the buttons except the “Initialization” button will be grey before clicking on Initialization, the
three buttons in the “Sample probe horizontal position” column will be illuminated and available for use Only
after clicking on Initialization. The “Middle position” and “Down position” buttons will not be available until
clicking on the “WBC bath” or “RBC bath” button.

Preheating temperature calibration and verification
Fill in the sum of the machine

measurement value and the FRU
value
Machine
measurement
value
Quality Control
Operation
Operation Content
Procedure QC Point QC Standard
Click the Start Menu bar, then click
“Service→ Temp.& Press.
Calibration” to enter the
2.1 “Temperature calibration” screen.
Click on the “Preheat bath
temperature calibration” button to
enter the screen shown in Figure 2.1
Record the machine measurement Measure the
value, and enter the sum of the preheat bath
2.2 machine measurement value and the temperature
value on the service FRU as the calibration factor
meter measurement value

Mechanical system—matters need attention
 All parts of the analyzer are potential infectious, safety protection measures
should be taken during operation and maintenance
 Reagents may irritate eyes, skin and mucous membrane. Operator who
contact such items as reagent in the laboratory, should comply with the
laboratory safety operating rules, and wear personal protective outfit (such as
laboratory protective clothing, gloves, etc.)
 Once the skin is in contact with Reagent, wash immediately with plenty of
water, if necessary, please go to see a doctor; Once the reagent is splashed
into your eyes, rinse immediately with plenty of water, and treated by doctors
when removing some component with electrostatic sensitive sign, electrostatic
ring or static gloves and other protective equipment need to be equipped to
avoid damage to the parts
 Choose the correct specifications and models of tube and screw
 keep the original tube length. Any prolongation and shortening are strictly
forbidden.
 Pay attention that the tube and cable could not bend or squeeze, when reinstall, to
prevent block or short circuit

Thanks ！

BC5000¡ BC5150 Service Training Material1.0

Uploaded by

Copyright:

Available Formats

You might also like

BC5000¡ BC5150 Service Training Material1.0

Uploaded by

Document Information

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

BC5000¡ BC5150 Service Training Material1.0

Uploaded by

Copyright:

Available Formats

BC-5000/BC5150 service training

© 2013 Mindray Confidential

2 © 2013 Mindray Confidential

 Blood consists of 40-50% blood cell and 50-60% plasma.

3 © 2013 Mindray Confidential

Five groups of leukocyte

WBC 5-part proportion

4 © 2013 Mindray Confidential

 Morphology ： size, shape, cytomembrane, cytoplasma, nucleus,

RBC WBC PLT

5 © 2013 Mindray Confidential

6 © 2013 Mindray Confidential

7 © 2013 Mindray Confidential

NEU EOS BASO LYMPH MONO

Cytoplasm Large more more less More than

8 © 2013 Mindray Confidential

Neutral Eosinophilic basophilic Azurophilic

Shape Fine particles Round or ellipse Differ in shape Differ in shape

Amount Much Much Less A few or

Distribution Even Even Uneven, coat the Uneven, coat the

9 © 2013 Mindray Confidential

10 © 2013 Mindray Confidential

11 © 2013 Mindray Confidential

12 © 2013 Mindray Confidential

WBC Eosinophils number Eos# Mean Corpuscular Hemoglobin Concentration MCHC

Monocytes percentage Mon% Hematocrit HCT

Platelet count PLT

Mean Platelet Volume MPV

Platelet-large cell count P-LCC

13 © 2013 Mindray Confidential

Abnormal lymphocytes number ALY#

Abnormal lymphocytes Percentage ALY%

Large immature cells number LIC#

Large immature cells percentage LIC%

14 © 2013 Mindray Confidential

 Front of the analyzer

15 © 2013 Mindray Confidential

1 、 M-52D diluent inlet

 Back of the analyzer

17 © 2013 Mindray Confidential

item BC-5000 BC-5150

18 © 2013 Mindray Confidential

 Measuring mode ： CBC 、 CBC+DIFF

19 © 2013 Mindray Confidential

 Operating temperature ： 10℃—30℃

20 © 2013 Mindray Confidential

Reagent Name Type

Diluent M-52D DILUENT M-52D

M-52DIFF LYSE M-52DIFF

Probe cleanser PROBE CLEANSER /

Control : Mindray made

21 © 2013 Mindray Confidential

22 © 2013 Mindray Confidential

23 © 2013 Mindray Confidential

24 © 2013 Mindray Confidential

25 © 2013 Mindray Confidential

Flow cell Scatter light

2ed Sheath flow 1st sheath flow