Apoptotic effects of CKD-602 and Cisplatin on oral squamous cell

carcinoma cell lines

Control Cisplatin Cisplatin Cis 5ug/ml + CKD-602 CKD-602

1ug/ml 5ug/ml CKD 0.5ug/ml 0.1ug/ml 0.5ug/ml

3.6 % 5.8 % 6.7 % 13.2%

YD-8

13.2 % 9.0 % 24.6 71.6 % 7.1 % 37.1 %

%
YD-9

8.8% 8.2% 19.7 % 59.5 %

YD-38
 YD-8

Control Cisplatin Cisplatin Cis 5ug/ml + CKD-602 CKD-602

1ug/ml 5ug/ml CKD 0.5ug/ml 0.1ug/ml 0.5ug/ml

3.6 % 5.8 % 6.7 % 13.2%

 YD-38

Control Cisplatin Cisplatin Cis 5ug/ml + CKD-602 CKD-602

1ug/ml 5ug/ml CKD 0.5ug/ml 0.1ug/ml 0.5ug/ml

8.8% 8.2% 19.7 % 59.5 %

 Cell cycle analysis

YD-8 (cisplatin 1ug/ml) YD-8 (CKD 0.1ug/ml)

100% 100%

Cell distribution (%)

Cell distribution (%)

80% 80%
G2/M G2/M
60% S 60% S
G1 G1
40% 40%

20% 20%

0% 0%
cont 24h 48h cont 24h 48h
YD-9 (cisplatin 1ug/ml) YD-9 (CKD 0.1ug/ml)
100% 100%

Cell distribution
Cell distribution (%)

80% G2/M 80% G2/M

60% S 60% S

(%)
G1 G1
40% 40%
20% 20%
0% 0%
cont 24h cont 24h

YD-38 (CKD 0.1ug/ml)

YD-38 (cisplatin 1ug/ml)
100%
Cell distribution

Cell distribution

100%
80% G2/M
80% G2/M
60% S
S
(%)

60%
(%)

G1
40% G1 40%

20% 20%
0% 0%
cont 24h cont 24h
Cell cycle analysis 2012.11.28

YD-8 YD-8
100%
Cell distribution (%)

100%

Cell distribution (%)

80%
80%
60% G2/M
60%
40% S
40%
G2/M G1
20%
S 20%
0%
cont 0.5 1 G1 0%
cont 0.02 0.1

Cisplatin (ug/ml) , 48hrs CKD602 (ug/ml), 48hrs

YD-38 YD-38
100% 100%
Cell distribution (%)

Cell distribution (%)

80% 80%
60% G2/M 60% G2/M
S S
40% 40%
G1 G1
20% 20%
0% 0%
cont 0.5 1 cont 0.02 0.1

Cisplatin (ug/ml) , 48hrs

CKD602 (ug/ml), 48hrs