Basic Concept of Animal Handling

Handling (Bhardwaj, 2016) Mouse

• Feeding and Watering
• Petting
Handling and Restraint
• Cleaning
• Manipulating Mice’s small body size
• Loading make them especially susceptible
• Crating to physical injury which might
• Shifting include inadvertently dropping by
• Transferring the handler when bitten by the
• Immobilizing mice. Some mice are intensely
• Treating active and may try to jump away
• Training from the handler.
• Working and Moving
 Grasped by the tail, preferably the proximal third and
lifted clear of its cage.
 Then, placed on a surface, for example, a cage top.
 The scruff can be griped in between the thumb and
forefinger whilst, retaining a grasp on the mice’s tail.
The mice is now secure and can be
examined or injected safely.

Handling for Injection

Intraperitoneal Injection Subcutaneous Injection

- Injections made into - Injections made

the posterior quadrant into the scruff of
of the abdomen. the neck.
Laws and Regulation in Animal Testing
(Science, Medicine and Animals, 2004)
Animal Welfare Act
- First federal law passed by Congress in
1966
- Covered transport, sale and handling of
animals
- Provided for licensing of animal dealers
- To prevent pet theft and sale to research
facilities
- Protects warm-blooded animals
- Rats, mice and birds which are common
species laboratory animals are excluded.
The Public Health Service Policy on
Humane Care and Use of Laboratory
Animals (PHS POLICY)
 Apply to any research facility that receives PHS funds.
(universities and colleges that perform animal
research)
 Provides a written plan for complying with PHS Policy
and the Guide for the Care and Use of Laboratory
Animals (The Guide).
 Research institution must have the IACUC review all
experimental procedure and inspect the facility.
 Loss of AAALAC accreditation and violations will
cause losing of PHS funding.
 Protect all vertebrate animals, including fish and
reptiles.
Institutional Animal Care and Use Committee (IACUC)

 Review all proposed animal experiments.

 Animal protocol must include:
1. Justification for using animals, the number of animals to be used and the species chosen.
2. The procedures or drugs to be used to eliminate or minimize pain and discomfort.
3. Description of the methods and sources used to search for alternatives to painful procedures.
4. Description of the search used to ensure that the experiment does not unnecessarily duplicate previous research.
 Members include: (at least 5)
1. A veterinarian – animal care
2. A scientist experienced in animal research
3. A professional not involved in research (e.g. lawyer, ethicist)
4. A member who is not associated with the institution
5. A representative of the interest of the community
 Examine animal facilities twice a year to make sure that the institution is in compliance with federal regulatory policy.
Association for Assessment and Accreditation
of Laboratory Animal Care International
(AAALAC INTERNATIONAL)