LABORATORY ACTIVITY 1

THE MICROSCOPE
 LEARNING OUTCOMES

AT THE END OF THE LABORATORY EXERCISE, THE STUDENT SHOULD BE ABLE TO:
• IDENTIFIED THE PARTS OF THE MICROSCOPE
• DESCRIBED THE FUNCTION OF EACH PART
• CALCULATE THE TOTAL MAGNIFICATION OF THE MICROSCOPE
• LEARNED PROPER HANDLING, CARE, AND MANIPULATION OF THE MICROSCOPE
 MICROSCOPE MAINTENANCE: BEST
PRACTICES
• CLEANING THE LENSES WITH LENS PAPER
• NOT ALLOWING LENSES TO CONTACT THE SLIDE (E.G., BY RAPIDLY CHANGING THE FOCUS)
• PROTECTING THE BULB (IF THERE IS ONE) FROM BREAKAGE
• NOT PUSHING AN OBJECTIVE INTO A SLIDE
• NOT USING THE COARSE FOCUSING KNOB WHEN USING THE 40× OR GREATER OBJECTIVE LENSES
• ONLY USING IMMERSION OIL WITH A SPECIALIZED OIL OBJECTIVE, USUALLY THE 100× OBJECTIVE
• CLEANING OIL FROM IMMERSION LENSES AFTER USING THE MICROSCOPE
• CLEANING ANY OIL ACCIDENTALLY TRANSFERRED FROM OTHER LENSES
• COVERING THE MICROSCOPE OR PLACING IT IN A CABINET WHEN NOT IN USE
 Parts Descriptions and Functions Classification
(Mechanical,
Magnifying,
   
Illuminating)
 
Eyepiece or Ocular
   

 
Body Tube
   

 
Arm
   

 
Scanner
   

 
Low Power Objective
   

 
High Power Objective
   

 
Oil Immersion Objective
    

 
Stage Clip    

 
Stage    

 
Illuminator    

 
Iris Diaphragm    

 
Revolving Nosepiece    

 
Coarse Adjustment
Knob    

 
Fine Adjustment Knob    

 
Base
 TOTAL MAGNIFICATION
LENSES MAGNIFICATION TOTAL MAGNIFICATION
OCULAR 10X
SCANNER 4X 40X
LOW POWER 10X 100X
HIGH POWER 40X 400X
OIL IMMERSION 100X 1000X
• EXPLAIN THE PROPER TECHNIQUE ON HOW TO HANDLE, AND CARE THE
COMPOUND MICROSCOPE.
• THE DEGREE TO WHICH THE IMAGE OF A SPECIMEN IS ENLARGED BY A
MICROSCOPE IS TERMED AS ______
• EXPLAIN WHY YOU SHOULD NOT USE THE COARSE ADJUSTMENT KNOB FOR
FOCUSING WHEN THE HIGH POWER OBJECTIVE OR OIL IMMERSION OBJECTIVE
ARE BEING USED?
• THE MAGNIFICATION OF THE SCANNER IS 4X, OCULAR LENS AND LOW POWER
OBJECTIVE ARE 10X, THE HIGH POWER OBJECTIVE IS 40X, AND THE OIL
IMMERSION OBJECTIVE IS 100X. WHAT WOULD BE THE TOTAL MAGNIFICATION OF
EACH OF THE LENSES?
• IF YOU HAVE BEEN ASKED TO PREPARE A SLIDE WITH THE LETTER Ë” ON IT,
WHAT WOULD BE THE APPEARANCE OF LETTER Ë” SEEN IN THE LOW POWER
OBJECTIVE AND HIGH POWER OBJECTIVE?
 DEFINE THE FOLLOWING

• MAGNIFICATION
• TOTAL MAGNIFICATION
• RESOLUTION
• PARFOCAL
• NUMERICAL APERTURE
LAB ACTIVITY 2.1
THE CELL ANATOMY
 LEARNING OUTCOMES

• IDENTIFIED THE PARTS OF THE CELL AND DESCRIBED THEIR FUNCTIONS

• DISTINGUISHED THE FOUR TYPES OF CELL BASED ON ITS STRUCTURE AND
FUNCTION.
3 MAJOR PARTS OF THE CELL
 GROUP 1

• AGOMO-O
• ALTURA, FRANCINE
• ALTURA, NINA
• ARGALLON
• BACALLA
 GROUP 2

• BAJO
• BASAMOT
• BOLIVAR
• BRETANA
• CABERO
 GROUP 3

• CALAYO
• CAMINIAN
• CARPIO
• GALLEGO
• YSULAN
 GROUP 4

• HARDER
• HIPONIA
• HUESCA
• IGNACIO
• ISIP, JR.
 GROUP 5

• ASIS
• LAURENTE
• LEGASPI
• MIRANDA
• NADALES
 GROUP 6

• NIEBLA
• OCTAVIO
• OSUMO
• PELINGON
• PIO
 GROUP 7

• REDISON
• SARMIENTO
• SOQUINA
• TARRAZONA
• TRAVINA
 GROUP 8

• TRIO
• TUPAZ
• UMADHAY
• VILLANUEVA